Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells

Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction

“…FN and Col-I, structural constitutes of ECM, were observed in each group, which were different from our previous observation on pancreatic β-cells. 24 Mechanisms underlying this distinction is not clear, but likely due to the difference between cell types, which we believe warrants an in-depth investigation in the further studies.…”

“…22,35 GelMA ratio not only affected the adhesion, but also altered the hydrogel mechanical properties. 24 We previously developed 3D culture systems for pancreatic β cells and cancer cells by adjusting mechanical properties of hydrogels. 22,[24][25][26] Based on these results, culture platforms specifically for DPSCs monolayer and spheroids were established in this study.…”

“…24 We previously developed 3D culture systems for pancreatic β cells and cancer cells by adjusting mechanical properties of hydrogels. 22,[24][25][26] Based on these results, culture platforms specifically for DPSCs monolayer and spheroids were established in this study. The shorter stress relaxation time and lower Young's modulus of hydrogel for spheroids formation indicated it was viscoelastic and soft, while the hydrogel was more elastic and rigid for MLCs formation, supported by longer stress relaxation time and higher Young's modulus.…”

“…By adjusting the composition of this platform, we designed specific mechanical microenvironments for a variety of cell types, such as fibroblasts, pancreatic βcells and cancer cells, in order to promote those cells yielding unique biological output with respect to cellular function, signal transduction, as well as response to therapeutic intervention. 22,[24][25][26][27] In this study, we designed two types of microenvironments for DPSCs with different mechanical properties based on the hydrogel platform to form monolayer cells (MLCs) or MCSs. In each condition, MLCs or MCSs formed as we expected, and cell morphology, viability, ECM molecules, surface markers and genes expression related to osteogenic/odontogenic differentiation were then examined with or without mechanical/magnetic stimulation of IONPs.…”

Degradable allylAntheraea pernyisilk fibroin thermoresponsive hydrogels to support cell adhesion and growth

“…Protein-based hydrogels have been recently pursued as important materials for biological scaffolds in tissue engineering, 1 cell culture, 2 and articial cartilage 3,4 because of natural structural proteins displaying critical structural and bioactive properties that have evolved in nature for millions of years. 5 Most of these hydrogels are highly similar to the native extracellular matrix in terms of bioactivity, and unique degradation and exible mechanical properties provide the opportunity to achieve various biological functions that are benecial for cell engineering.…”