Three-dimensional cell aggregates composed of HUVECs and cbMSCs for therapeutic neovascularization in a mouse model of hindlimb ischemia

Chen, Ding-Yuan; Wei, Hao-Ji; Lin, Kun-Ju; Huang, Chieh‐Cheng; Wang, Chung-Chi; Wu, Cheng‐Tse; Chao, Ko-Ting; Chen, Ko-Jie; Chang, Yen; Sung, Hsing‐Wen

doi:10.1016/j.biomaterials.2012.11.045

Cited by 44 publications

(45 citation statements)

References 42 publications

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“…Tissue engineering-based research in both two-dimensional (2D) and three-dimensional cocultures in vitro revealed that the active interaction between ECs and MSCs (or osteoprogenitor and fibroblast) induced the formation of tube-like cell aggregation structures. This result indicates that angiogenesis and prevascularization are involved in the cellcell communication and remodeling of the functional blood vessel development in vivo [5,[18][19][20][21][22][23][24][25][26]. Moreover, MSCs could repress ECs by regulating cytokine-induced leukocyte recruitment [27], while the activated ECs could regulate the MSCs-to-ECs transmigration in a leukocyte-like mechanism [28,29].…”

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confidence: 99%

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

LiJunxiang

MaYing

TengRuifang

et al. 2015

Stem Cells and Development

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Mesenchymal stem cells (MSCs) show great promise in blood vessel restoration and vascularization enhancement in many therapeutic situations. Typically, the co-implantation of MSCs with vascular endothelial cells (ECs) is effective for the induction of functional vascularization in vivo, indicating its potential applications in regenerative medicine. The effects of MSCs-ECs-induced vascularization can be modeled in vitro, providing simplified models for understanding their underlying communication. In this article, a contact coculture model in vitro and an RNA-seq approach were employed to reveal the active crosstalk between MSCs and ECs within a short time period at both morphological and transcriptional levels. The RNA-seq results suggested that angiogenic genes were significantly induced upon coculture, and this prevascularization commitment might require the NF-kB signaling. NF-kB blocking and interleukin (IL) neutralization experiments demonstrated that MSCs potentially secreted IL factors including IL1b and IL6 to modulate NF-kB signaling and downstream chemokines during coculture. Conversely, RNA-seq results indicated that the MSCs were regulated by the coculture environment to a smooth muscle commitment within this short period, which largely induced myocardin, the myogenic co-transcriptional factor. These findings demonstrate the mutual molecular mechanism of MSCs-ECs-induced prevascularization commitment in a quick response.

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mentioning

confidence: 99%

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

LiJunxiang

MaYing

TengRuifang

et al. 2015

Stem Cells and Development

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“…The transplantation of various types of cells with angiogenic potential, such as endothelial progenitor cells or stem cells, has shown great promise for treating peripheral arterial disease by the stimulation of new vessel formation [21,22]. However, conventional methods for the delivery of therapeutic cells rely on the direct or systemic injection of cell suspensions with a syringe needle, which is problematic due to the fast leakage of suspended cells, cell death, and low engraftment efficiency [9,23e25].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic angiogenesis by a myoblast layer harvested by tissue transfer printing from cell-adhesive, thermosensitive hydrogels

et al. 2013

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“…In this pilot study, a dose-dependent positive correlation was seen with limb reperfusion and the cell mass injected into the adductor muscle of a PAD mouse model, but especially pronounced in diabetic mice. Innovative approaches based on tissue transferprinting [120] of myoblast monolayers as well as three-dimensional aggregates of human umbilical vein endothelial cells and cord-blood mesenchymal stem cells [121] have demonstrated promise towards hastening tube formation.…”

Section: Future Directions and Considerationsmentioning

confidence: 99%

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

Balikov

Lee

Boire

et al. 2015

Biosystems &Amp; Biorobotics

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Because of their potential to regenerate tissues and organs, stem cells garner extensive interest worldwide for treating a wide range of degenerative diseases. In numerous efforts, a variety of stem cell formats have been considered for therapeutic purposes to combat such pathologies, one being vascular-related diseases. In particular, the prevalence of peripheral artery disease (PAD) has steadily increased with the growth of the aging population in many first-world countries. Considering this disturbing trend as well as the obesity epidemic and the ballooning population growth in third-world nations, the burden of PAD is expected to increase worldwide to alarming levels in the coming decades. The advent of stem cell treatments could stymie this burden by alleviating the complications and improving upon the less-than-satisfactory outcomes from current standard-of-care surgical/pharmacological interventions for PAD. This chapter reviews the relevant, cutting-edge clinical and animal model research efforts in the field and explores the remaining questions as they pertain to convergence technologies that may provide the potential for stem cells to reverse PAD-induced tissue damage. Harnessing and translating this potential to create more viable and efficacious PAD treatments should be of paramount global and public health concern.

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Three-dimensional cell aggregates composed of HUVECs and cbMSCs for therapeutic neovascularization in a mouse model of hindlimb ischemia

Cited by 44 publications

References 42 publications

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

Therapeutic angiogenesis by a myoblast layer harvested by tissue transfer printing from cell-adhesive, thermosensitive hydrogels

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

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