Three-dimensional bioprinting of tissues and organs

Pai, Roopesh R.; Sekar, J. Anupama; Ajit, Shilpa; Velayudhan, D. Shiny; Kasoju, Naresh; P.R., Anil Kumar

doi:10.1016/b978-0-12-823966-7.00018-9

Cited by 3 publications

(1 citation statement)

References 52 publications

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“…Its advantages include improved accuracy and structural support, enabling the creation of complex structures. Challenges of this approach encompass enhancing cell survival, promoting proper cell interactions, and ensuring efficient nutrient delivery within the printed structures [49][50][51][52].…”

Section: Methodsmentioning

confidence: 99%

Introduction of Hybrid Additive Manufacturing for Producing Multi-Material Artificial Organs for Education and In Vitro Testing

Chatzipapas,

Nika,

Krimpenis

2024

Designs

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The evolution of 3D printing has ushered in accessibility and cost-effectiveness, spanning various industries including biomedical engineering, education, and microfluidics. In biomedical engineering, it encompasses bioprinting tissues, producing prosthetics, porous metal orthopedic implants, and facilitating educational models. Hybrid Additive Manufacturing approaches and, more specifically, the integration of Fused Deposition Modeling (FDM) with bio-inkjet printing offers the advantages of improved accuracy, structural support, and controlled geometry, yet challenges persist in cell survival, interaction, and nutrient delivery within printed structures. The goal of this study was to develop and present a low-cost way to produce physical phantoms of human organs that could be used for research and training, bridging the gap between the use of highly detailed computational phantoms and real-life clinical applications. To this purpose, this study utilized anonymized clinical Computed Tomography (CT) data to create a liver physical model using the Creality Ender-3 printer. Polylactic Acid (PLA), Polyvinyl Alcohol (PVA), and light-bodied silicone (Polysiloxane) materials were employed for printing the liver including its veins and arteries. In brief, PLA was used to create a mold of a liver to be filled with biocompatible light-bodied silicone. Molds of the veins and arteries were printed using PVA and then inserted in the liver model to create empty channel. In addition, the PVA was then washed out by the final product using warm water. Despite minor imperfections due to the printer’s limitations, the final product imitates the computational model accurately enough. Precision adjustments in the design phase compensated for this variation. The proposed novel low-cost 3D printing methodology successfully produced an anatomically accurate liver physical model, presenting promising applications in medical education, research, and surgical planning. Notably, its implications extend to medical training, personalized medicine, and organ transplantation. The technology’s potential includes injection training for medical professionals, personalized anthropomorphic phantoms for radiation therapy, and the future prospect of creating functional living organs for organ transplantation, albeit requiring significant interdisciplinary collaboration and financial investment. This technique, while showcasing immense potential in biomedical applications, requires further advancements and interdisciplinary cooperation for its optimal utilization in revolutionizing medical science and benefiting patient healthcare.

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Section: Methodsmentioning

confidence: 99%

Introduction of Hybrid Additive Manufacturing for Producing Multi-Material Artificial Organs for Education and In Vitro Testing

Chatzipapas,

Nika,

Krimpenis

2024

Designs

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Comprehensive sustainable development of a multifunctional machine: 3D food printer and didactic platform

Molina,

Cortés,

Chairez

et al. 2024

International Journal of Sustainable Engineering

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Scaffold-mediated liver regeneration: A comprehensive exploration of current advances

Bhatt S,

Krishna Kumar,

Laya

et al. 2024

J Tissue Eng

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The liver coordinates over 500 biochemical processes crucial for maintaining homeostasis, detoxification, and metabolism. Its specialized cells, arranged in hexagonal lobules, enable it to function as a highly efficient metabolic engine. However, diseases such as cirrhosis, fatty liver disease, and hepatitis present significant global health challenges. Traditional drug development is expensive and often ineffective at predicting human responses, driving interest in advanced in vitro liver models utilizing 3D bioprinting and microfluidics. These models strive to mimic the liver’s complex microenvironment, improving drug screening and disease research. Despite its resilience, the liver is vulnerable to chronic illnesses, injuries, and cancers, leading to millions of deaths annually. Organ shortages hinder liver transplantation, highlighting the need for alternative treatments. Tissue engineering, employing polymer-based scaffolds and 3D bioprinting, shows promise. This review examines these innovative strategies, including liver organoids and liver tissue-on-chip technologies, to address the challenges of liver diseases.

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Three-dimensional bioprinting of tissues and organs

Cited by 3 publications

References 52 publications

Introduction of Hybrid Additive Manufacturing for Producing Multi-Material Artificial Organs for Education and In Vitro Testing

Introduction of Hybrid Additive Manufacturing for Producing Multi-Material Artificial Organs for Education and In Vitro Testing

Comprehensive sustainable development of a multifunctional machine: 3D food printer and didactic platform

Scaffold-mediated liver regeneration: A comprehensive exploration of current advances

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