Three-dimensional bioluminescence tomography with model-based reconstruction

Gu, Xuejun; Zhang, Qizhi; Larcom, Lyndon L.; Jiang, Huabei

doi:10.1364/opex.12.003996

Cited by 124 publications

(88 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, Wang et al described the BLT principles and reported the uniqueness of solution dependent on a priori knowledge [14,15]. Partial numerical studies were also reported in the recent literature [16,17]. In the present work, we develop a novel reconstruction algorithm to identify the bioluminescent source distribution from the measured external photon density.…”

Section: Introductionmentioning

confidence: 92%

Practical reconstruction method for bioluminescence tomography

Cong¹,

Wang²,

Kumar³

et al. 2005

Opt. Express

282

314

View full text Add to dashboard Cite

Bioluminescence tomography (BLT) is used to localize and quantify bioluminescent sources in a small living animal. By advancing bioluminescent imaging to a tomographic framework, it helps to diagnose diseases, monitor therapies and facilitate drug development. In this paper, we establish a direct linear relationship between measured surface photon density and an unknown bioluminescence source distribution by using a finite-element method based on the diffusion approximation to the photon propagation in biological tissue. We develop a novel reconstruction algorithm to recover the source distribution. This algorithm incorporates a priori knowledge to define the permissible source region in order to enhance numerical stability and efficiency. Simulations with a numerical mouse chest phantom demonstrate the feasibility of the proposed BLT algorithm and reveal its performance in terms of source location, density, and robustness against noise. Lastly, BLT experiments are performed to identify the location and power of two light sources in a physical mouse chest phantom.

show abstract

Section: Introductionmentioning

confidence: 92%

Practical reconstruction method for bioluminescence tomography

Cong¹,

Wang²,

Kumar³

et al. 2005

Opt. Express

282

314

View full text Add to dashboard Cite

show abstract

“…BL tomography (BLT) would give the ability to reconstruct cross-sectional slices through the BL site(s) and recover depth and spatial resolution of BL sources and could significantly enhance contrast resolution and quantitative accuracy. However, BLT requires the incorporation of an accurate photon migration model into the reconstruction process to account for light absorption and scatter along any given path of the photons [33,34]. For tomographic imaging capabilities, since the surface radiance strongly depends upon the tissue thickness that the light traverses, a special light source should be available to measure the 3D contours of the surface of the animal.…”

Section: Bl Tomography?mentioning

confidence: 99%

“…These properties will provide more useful correlation to images from other modalities, such as PET. However, the very low BL quantum yield, high scatter and absorption, and significant tissue heterogeneity make in vivo BLT an extremely challenging problem [33,34].…”

Section: Bl Tomography?mentioning

confidence: 99%

Primer on molecular imaging technology

Levin

2005

Eur J Nucl Med Mol Imaging

109

View full text Add to dashboard Cite

Abstract. A wide range of technologies is available for in vivo, ex vivo, and in vitro molecular and cellular imaging. This article focuses on three key in vivo imaging system instrumentation technologies used in the molecular imaging research described in this special issue of Eur J Nucl Med Mol Imaging: positron emission tomography, singlephoton emission computed tomography, and bioluminescence imaging. For each modality, the basics of how it works, important performance parameters, and the state-ofthe-art instrumentation are described. Comparisons and integration of multiple modalities are also discussed. The principles discussed in this article apply to both human and small animal imaging.

show abstract

“…In addition, optical imaging has several other advantages over the established modalities [17][18][19]: the use of low-energy electromagnetic waves in the nearinfrared range poses no risk of ionizing radiation. Optical imaging can provide spectroscopic information on tissue composition [20,21].…”

Section: Biophotonicsmentioning

confidence: 99%

Near‐infrared imaging of breast cancer using optical contrast agents

Poellinger

2012

Journal of Biophotonics

View full text Add to dashboard Cite

Breast cancer is the most common malignancy in women worldwide and the second leading cause of cancer death. On the basis of three studies performed by our group, this article reviews the current status of optical breast imaging using extrinsic contrast agents. To date, only two contrast agents have been applied in human studies, indocyanine green (ICG) and omocianine. Both contrast media were used for absorption and fluorescence imaging. Generally speaking, malignant breast lesions exhibited higher absorption contrast as well as higher fluorescence contrast compared to benign lesions or non‐diseased breast tissue. Some groups consider early enhancement characteristics helpful for differentiation between malignant and benign lesions. Late fluorescence ICG imaging – capitalizing on the extravasation of the dye through the wall of tumorous vessels – seems to be a promising technique to distinguish malignant from benign breast lesions. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

Three-dimensional bioluminescence tomography with model-based reconstruction

Cited by 124 publications

References 1 publication

Practical reconstruction method for bioluminescence tomography

Practical reconstruction method for bioluminescence tomography

Primer on molecular imaging technology

Near‐infrared imaging of breast cancer using optical contrast agents

Contact Info

Product

Resources

About