Three-dimensional bio-printing

Gu, Qi; Hao, Jie; Lu, YangJie; Wang, Liu; Wallace, Gordon G.; Zhou, Qi

doi:10.1007/s11427-015-4850-3

Cited by 78 publications

(45 citation statements)

References 93 publications

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“…This method allows for optimizing the scaffold properties at two different levels to better mimic biological cartilage tissues. In addition, PLGA has good biocompatibility; 39 the cross‐linking agent genipin is a natural extract, suitable for biological material treatment; dECM used in this research is a natural pig cartilage extract, displaying better biological activity . These multimaterial scaffolds can be used in in vitro cell experiments and in vivo transplantation experiments.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In addition, hydrogels, such as decellularized extracellular matrix (dECM), collagen, gelatin, with cartilage stem cells and growth factors added were proven to have biological activity suitable for stem cell growth and tissue regeneration, but their mechanical properties were weak. [11][12][13][14][15][16][17][18][19] By freeze drying, both of these materials can be made into porous structures 17,18 beneficial to cell growth. In further research, by gradient or graded structural designing and oriented freezing, the scaffolds' mechanical properties can be enhanced and bionically optimized.…”

Section: Introductionmentioning

confidence: 99%

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Construction of bionic tissue engineering cartilage scaffold based on three‐dimensional printing and oriented frozen technology

Guo

Yang

et al. 2018

J Biomedical Materials Res

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Articular cartilage (AC) has gradient features in both mechanics and histology as well as a poor regeneration ability. The repair of AC poses difficulties in both research and the clinic. In this paper, a gradient scaffold based on poly(lactic-co-glycolic acid) (PLGA)-extracellular matrix was proposed. Cartilage scaffolds with a three-layer gradient structure were fabricated by PLGA through three-dimensional printing, and the microstructure orientation and pore fabrication were made by decellularized extracellular matrix injection and directional freezing. The manufactured scaffold has a mechanical strength close to that of real cartilage. A quantitative optimization of the Young's modulus and shear modulus was achieved by material mechanics formulas, which achieved a more accurate mechanical bionic and a more stable interface performance because of the one-time molding process. At the same time, the scaffolds have a bionic and gradient microstructure orientation and pore size, and the stratification ratio can be quantitatively optimized by design of the freeze box and temperature simulation. In general, this paper provides a method to optimize AC scaffolds by both mechanics and histology as well as a bionic multimaterial scaffold. This paper is of significance for cell culture and clinical transplantation experiments. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1664-1676, 2018.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction of bionic tissue engineering cartilage scaffold based on three‐dimensional printing and oriented frozen technology

Guo

Yang

et al. 2018

J Biomedical Materials Res

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“…hilst 2D printing has had a big influence on everyday living, the advent of additive processing technology in 1986 [1] has seen an explosion in innovative ways of producing 3D structures, such as electronic devices [2] , aircraft parts [3] , medical devices [4] and tissue mimics [5][6][7] . For clinical applications, early designs based on creating sacrificial moulds as templates for the biomaterials [8] were quickly superseded by aqueous systems that could directly print biological materials [9−11] .…”

Section: Introductionmentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.

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“…In summary, the relationship between 3D conditions and stem cell has attracted considerable attentions (Gu et al, 2015). Our study may provide a novel and useful avenue for stem cell research.…”

mentioning

confidence: 99%