Three-dimensional analysis of the developing pituitary gland in the mouse

Hashimoto, Hisashi; Ishikawa, Hiroshi; Kusakabe, Moriaki

doi:10.1002/(sici)1097-0177(199805)212:1<157::aid-aja14>3.0.co;2-4

Cited by 16 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S1 A and B). Laminin, an ECM glycoprotein that is bound by a subset of integrin β1 heterodimers, was abundant in the basement membrane that separates the epithelial tissue of the early embryonic pituitary gland from adjacent mesenchyme (3,14). As angiogenesis proceeds, this demarcation persists in the form of a vascular basement membrane that surrounds nascent vessels in the anterior lobe (3) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…5C). Curiously, integrins α3β1 and α7β1 bind specifically to laminin, a major component of vascular basement membranes in the embryonic pituitary (3,14).…”

Section: Rna-sequencing Identified An Integrin β1-dependent Transcripmentioning

confidence: 99%

“…A dense vascular network delivers hypothalamic regulatory factors and target organ feedback signals that control pituitary endocrine function (2). Angiogenesis gives rise to this network beginning on mouse embryonic day 13.5 (e13.5) as epithelial cells in the expanding anterior wall of Rathke's pouch delaminate and intercalate with invading endothelial and mesenchymal cells (3). As embryonic blood vessels branch and spread laterally throughout the expanding anterior lobe, they form fenestrated capillaries that surround developing endocrine cells (4).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland

Scully

Skowronska‐Krawczyk

Krawczyk

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

As a key component of the vertebrate neuroendocrine system, the pituitary gland relies on the progressive and coordinated development of distinct hormone-producing cell types and an invading vascular network. The molecular mechanisms that drive formation of the pituitary vasculature, which is necessary for regulated synthesis and secretion of hormones that maintain homeostasis, metabolism, and endocrine function, remain poorly understood. Here, we report that expression of integrin β1 in embryonic pituitary epithelial cells is required for angiogenesis in the developing mouse pituitary gland. Deletion of pituitary epithelial integrin β1 before the onset of angiogenesis resulted in failure of invading endothelial cells to recruit pericytes efficiently, whereas deletion later in embryogenesis led to decreased vascular density and lumen formation. In both cases, lack of epithelial integrin β1 was associated with a complete absence of vasculature in the pituitary gland at birth. Within pituitary epithelial cells, integrin β1 directs a large transcriptional program that includes components of the extracellular matrix and associated signaling factors that are linked to the observed non-cell-autonomous effects on angiogenesis. We conclude that epithelial integrin β1 functions as a critical and canonical regulator of developmental angiogenesis in the pituitary gland, thus providing insight into the long-standing systems biology conundrum of how vascular invasion is coordinated with tissue development.angiogenesis | integrin β1 | pituitary gland | mice

show abstract

Section: Resultsmentioning

confidence: 99%

“…5C). Curiously, integrins α3β1 and α7β1 bind specifically to laminin, a major component of vascular basement membranes in the embryonic pituitary (3,14).…”

Section: Rna-sequencing Identified An Integrin β1-dependent Transcripmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland

Scully

Skowronska‐Krawczyk

Krawczyk

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Vascularization of the pituitary gland is important for allowing hormones to be secreted and taken to target organs and to allow negative feedback signals to reach the pituitary gland (60). Despite the importance of vascular development for transport of hormones, there is still much to be learned about how this process is regulated in development (41,76,83). In particular, we are interested in whether the development of the vasculature stimulates pituitary cell differentiation as it does in the pancreas (52,53).…”

Section: Discussionmentioning

confidence: 99%

Candidate genes for panhypopituitarism identified by gene expression profiling

Mortensen¹,

MacDonald

Ghosh

et al. 2011

Physiological Genomics

View full text Add to dashboard Cite

Mutations in the transcription factors PROP1 and PIT1 (POU1F1) lead to pituitary hormone deficiency and hypopituitarism in mice and humans. The dysmorphology of developing Prop1 mutant pituitaries readily distinguishes them from those of Pit1 mutants and normal mice. This and other features suggest that Prop1 controls the expression of genes besides Pit1 that are important for pituitary cell migration, survival, and differentiation. To identify genes involved in these processes we used microarray analysis of gene expression to compare pituitary RNA from newborn Prop1 and Pit1 mutants and wild-type littermates. Significant differences in gene expression were noted between each mutant and their normal littermates, as well as between Prop1 and Pit1 mutants. Otx2, a gene critical for normal eye and pituitary development in humans and mice, exhibited elevated expression specifically in Prop1 mutant pituitaries. We report the spatial and temporal regulation of Otx2 in normal mice and Prop1 mutants, and the results suggest Otx2 could influence pituitary development by affecting signaling from the ventral diencephalon and regulation of gene expression in Rathke's pouch. The discovery that Otx2 expression is affected by Prop1 deficiency provides support for our hypothesis that identifying molecular differences in mutants will contribute to understanding the molecular mechanisms that control pituitary organogenesis and lead to human pituitary disease.

show abstract

“…rat embryo pituitary glands were isolated according to described methods [38] under a dissection microscope. They were subsequently placed in an Eppendorf tube and centrifuged at 5000 rpm for 1 min to eliminate the supernatant; the tissue was homogenized and sonicated in order to break the cellular membranes and liberate intracytoplasmic proteins.…”

Section: Il-1 and Il-6 Western-blotmentioning

confidence: 99%

Prenatal expression of interleukin 1β and interleukin 6 in the rat pituitary gland

et al. 2008

View full text Add to dashboard Cite

a b s t r a c tIt is known that interleukin 1b (IL-1b) and interleukin 6 (IL-6) are expressed post-natally in normal and tumoral cells in the anterior pituitary, and that they play a role in both the liberation of different hormones and in the growth, proliferation and tumor formation of the pituitary gland. However, their expression and role during embryonic and fetal development remain unknown. We have performed an immunocytochemistry study of prenatal expression and distribution of IL-1b and IL-6 in isolated embryonic rat Rathke's pouch prior to birth, more specifically between 13.5 and 19.5 days p.c. Western-blot analysis carried out on 19.5-day p.c. embryos showed positive immunolabelling for IL-1b and IL-6. These interleukins were initially expressed simultaneously in the rostral and ventral portions of Rathke's pouch in 15.5-day p.c. embryos, and this expression progressed caudodorsally in later developmental stages, extending to most of the hypophysis before birth. The number of cells expressing these interleukins increased throughout this period: 48.22% of anterior pituitary cells expressed IL-6 in 19.5-day embryos, whilst IL-1b was positive in 39.8% of the cells. Moreover, we have demonstrated that some adenohypophyseal cells co-express both interleukins. Such findings represent the first step towards an understanding of the physiological role of these interleukins in anterior pituitary development.

show abstract

Three-dimensional analysis of the developing pituitary gland in the mouse

Cited by 16 publications

References 16 publications

Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland

Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland

Candidate genes for panhypopituitarism identified by gene expression profiling

Prenatal expression of interleukin 1β and interleukin 6 in the rat pituitary gland

Contact Info

Product

Resources

About