Three dimensional (3D) analysis of the morphological changes induced by 50 Hz magnetic field exposure on human lymphoblastoid cells (Raji)

Lisi, Antonella; Pozzi, Deleana; Pasquali, Emanuela; Rieti, Sabrina; Girasole, M.; Cricenti, A.; Generosi, R.; Serafino, Annalucia; Congiu‐Castellano, A.; Ravagnan, G.; Giuliani, Livio; Grimaldi, Settimio

doi:10.1002/(sici)1521-186x(200001)21:1<46::aid-bem7>3.0.co;2-z

Cited by 44 publications

(26 citation statements)

References 19 publications

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“…Any disorganization of this surface morphology severely accelerates the entrance of ionic and lipophilic xenobiotics into the cytoplasm. Long-term exposure to MFs has been shown to completely abolish the microvillar morphology (57,76) of lymphocytes and embryonic neural endoderm (98). Thus the sensitizing effect of EMFs on the action of cytotoxic compounds can be used for treatment of drug-resistant tumors (30,51,88).…”

Section: Discussionmentioning

confidence: 99%

Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli

Gartzke

Lange²

2002

American Journal of Physiology-Cell Physiology

102

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Gartzke, Joachim, and Klaus Lange. Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli. Am J Physiol Cell Physiol 283: C1333-C1346, 2002; 10.1152/ajpcell. 00167.2002.-The interaction of weak electromagnetic fields (EMF) with living cells is a most important but still unresolved biophysical problem. For this interaction, thermal and other types of noise appear to cause severe restrictions in the action of weak signals on relevant components of the cell. A recently presented general concept of regulation of ion and substrate pathways through microvilli provides a possible theoretical basis for the comprehension of physiological effects of even extremely low magnetic fields. The actin-based core of microfilaments in microvilli is proposed to represent a cellular interaction site for magnetic fields. Both the central role of F-actin in Ca 2ϩ signaling and its polyelectrolyte nature eliciting specific ion conduction properties render the microvillar actin filament bundle an ideal interaction site for magnetic and electric fields. Ion channels at the tip of microvilli are connected with the cytoplasm by a bundle of microfilaments forming a diffusion barrier system. Because of its polyelectrolyte nature, the microfilament core of microvilli allows Ca 2ϩ entry into the cytoplasm via nonlinear cable-like cation conduction through arrays of condensed ion clouds. The interaction of ion clouds with periodically applied EMFs and field-induced cation pumping through the cascade of potential barriers on the F-actin polyelectrolyte follows well-known physical principles of ion-magnetic field (MF) interaction and signal discrimination as described by the stochastic resonance and Brownian motor hypotheses. The proposed interaction mechanism is in accord with our present knowledge about Ca 2ϩ signaling as the biological main target of MFs and the postulated extreme sensitivity for coherent excitation by very low field energies within specific amplitude and frequency windows. Microvillar F-actin bundles shielded by a lipid membrane appear to function like electronic integration devices for signal-tonoise enhancement; the influence of coherent signals on cation transduction is amplified, whereas that of random noise is reduced. calcium signaling; cell differentiation; Brownian motor hypothesis; cyclotron resonance; hair cell; mechanoelectrical coupling; physiological effects MORE THAN A DECADE OF RESEARCH on field effects in biological systems has yielded rather compelling data for the involvement of the Ca 2ϩ signaling pathway as a primary and main target of magnetic fields (MFs). As first demonstrated by Liburdy and colleagues (52,53) and later on by a number of other authors, the Ca 2ϩ influx pathways of isolated or cultured cells are severely affected by rather low MF energies.The physiological relevance of this finding is high, because Ca 2ϩ represents the most important intracellular signal governing almost all physiological functions in differentiated cells. Most importantly, cytopla...

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Section: Discussionmentioning

confidence: 99%

Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli

Gartzke

Lange²

2002

American Journal of Physiology-Cell Physiology

102

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“…Possible alterations in enzyme actions involved in cytotic processes in thyroicytes and hormone release should be taken into consideration. Results of experimental investigations that demonstrated the EMF effect on enzymes (Ding et al, 2001) and alterations in the structure and function of cellular membranes (Lisi et al, 2000;Bordiushkov et al, 2000) which are appointed as primary targets of EMF action on biological systems (Tenforde;Kaune, 1987;Goodman;Greenebaum, Marron, 1995), implicate the possible aspect of a direct EMF influence on the thyroid gland (MATAVULJ et al, 1998) Apud Rajkovic et al (2003. Furthermore Tonini et al, (2001) in their study demonstrated that a 50/60-Hz magnetic field interacts with cell differentiation through two opposing mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Electromagnetic field, 60 Hz, 3 µT, on the hormonal and metabolic regulation of undernourished pregnant rats

2009

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Epidemiological studies have implicated maternal protein-calorie deficiency as an important public health problem in developing countries. Over the last decades, a remarkable diffusion of electricity and an increased level of the electromagnetic field (EMF) in the environment have characterized modern societies. Therefore, researchers are concerned with the biological effects of 50-60 Hz, EMF. The aim of this paper is to show the effects of EMF of 60 Hz, 3 µT, exposure for two hours per day in the regulation of the hormonal and metabolic concentrations in pregnant rats, which were fed by Regional Basic Diet (RBD) during their pregnancy as compared with pregnant rats fed a standard diet. Pregnant rats exposed to EMF of 60 Hz, 3 µT, over the pregnancy and fed with RBD presented an increase in glucose release when compared with the Group subjected only to the RBD ration. Rats fed RBD presented a decrease in their insulin and cortisol serum levels when compared with the Group fed with casein. The T 3 and T 4 concentrations presented the greatest variation among the Groups. The relation T 4 :T 3 was much exaggerated in the Group subjected to RDB and exposed to EMF when compared to the others. In conclusion, the group subjected to the association of EMF and undernutrition suffered a decrease in its serum concentration of T 4 and T 3 when compared to the well-nourished group and the relationship T 4 :T 3 in the former group was almost eighteen-fold the later one.Keywords: casein, electromagnetic field, metabolism, hormone, regional basic diet, undernourished pregnant rats. Efeitos do campo eletromagnético, 60 Hz, 3µT, na regulação hormonal e metabólica de ratas prenhes desnutridas ResumoEstudos epidemiológicos têm mostrado que a deficiência proteico-calórica é um importante problema nos países em desenvolvimento. Durante as últimas décadas, a sociedade moderna tem sido caracterizada pelo aumento no número de equipamentos elétricos e como consequência um aumento no nível do campo eletromagnético (CEM) no ambiente. No entanto, os pesquisadores estão preocupados com os efeitos biológicos dos CEM de 50-60 Hz. O objetivo deste artigo é mostrar os efeitos do CEM de 60 Hz, 3 µT, nas concentrações hormonais e metabólicas de ratas prenhes, expostas duas horas por dia ao CEM, alimentadas pela Dieta Básica Regional (DBR) comparando com ratas submetidas às mesmas condições, mas alimentas com dieta padrão. Ratas prenhes expostas ao CEM de 60 Hz, 3 µT, durante a prenhez e alimentadas com a DBR apresentaram um aumento na liberação de glicose quando comparadas com o grupo alimentado pela DBR sem CEM. As ratas alimentadas pela DBR apresentaram uma diminuição nos níveis de insulina e cortisol quando comparadas com o grupo alimentado pela caseína. As concentrações de T 3 e T 4 apresentaram a maior variação entre os grupos. A relação T 4 :T 3 foi muito exagerada no grupo alimentado pela DBR e exposto ao CEM quando comparado com os outros. Conclusão, os animais que foram submetidos à desnutrição e ao CEM sofreram uma diminuição na ...

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“…But there is a supposition that the most sensitive element to the magnetic field action a cell plasmolemma due to its bioelectric properties [18,19]. It has been reported previously that SMF of medium intensity can change plasmolemma functions in different types of cells [20][21][22][23][24][25]. One of the most important functional characteristic is its ability to adhesion.…”

Section: Magnetic Field Influence On Cells Adhesion Morphology and Pmentioning

confidence: 99%

Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology

Ignatyeva¹,

Voyevodin²,

Goltsev³

et al. 2014

BIO

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Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.

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Three dimensional (3D) analysis of the morphological changes induced by 50 Hz magnetic field exposure on human lymphoblastoid cells (Raji)

Cited by 44 publications

References 19 publications

Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli

Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli

Effects of the Electromagnetic field, 60 Hz, 3 µT, on the hormonal and metabolic regulation of undernourished pregnant rats

Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology

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