Three-chamber Social Approach Task with Optogenetic Stimulation (Mice)

Leung, Celeste; Kim, Jun Chul; Jia, Zhengping

doi:10.21769/bioprotoc.3120

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…Social ability was evaluated by the three-chamber test 27 . This test included the social preference test and social memory test.…”

Section: Methodsmentioning

confidence: 99%

Decreased miR-451a in cerebrospinal fluid, a marker for both cognitive impairment and depressive symptoms in Alzheimer's disease

Feng¹,

Hu²,

Chen³

et al. 2023

Theranostics

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Background: Alzheimer's disease (AD) patients are often accompanied by depressive symptoms, but its underlying mechanism remains unclear. The present study aimed to explore the potential role of microRNAs in the comorbidity of AD and depression. Methods: The miRNAs associated with AD and depression were screened from databases and literature and then confirmed in the cerebrospinal fluid (CSF) of AD patients and different ages of transgenic APP/PS1 mice. AAV9-miR-451a-GFP was injected into the medial prefrontal cortex (mPFC) of APP/PS1 mice at seven months, and four weeks later, a series of behavioral and pathological analyses were performed. Results: AD patients had low CSF levels of miR-451a, which was positively correlated with the cognitive assessment score, but negatively with their depression scale. In the mPFC of APP/PS1 transgenic mice, the miR-451a levels also decreased significantly in the neurons and microglia. Specific virus vector-induced overexpression of miR-451a in the mPFC of APP/PS1 mice ameliorated AD-related behavior deficits and pathologies, including long-term memory defects, depression-like phenotype, β-amyloid load, and neuroinflammation. Mechanistically, miR-451a decreased the expression of neuronal β-secretase 1 of neurons through inhibiting Toll-like receptor 4/Inhibitor of kappa B Kinase β/ Nuclear factor kappa-B signaling pathway and microglial activation by inhibiting activation of NOD-like receptor protein 3, respectively. Conclusion: This finding highlighted miR-451a as a potential target for diagnosing and treating AD, especially for those with coexisting symptoms of depression.

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“…Social ability was evaluated by the three-chamber test 27 . This test included the social preference test and social memory test.…”

Section: Methodsmentioning

confidence: 99%

Decreased miR-451a in cerebrospinal fluid, a marker for both cognitive impairment and depressive symptoms in Alzheimer's disease

Feng¹,

Hu²,

Chen³

et al. 2023

Theranostics

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“…The subject mouse had a choice between the first, already investigated unfamiliar mouse (stranger (T1), familiar (T2)), and the novel unfamiliar mouse. The interaction was defined as the sniffing or direct contact when the subject animal oriented its nose or initiated physical contact within 2 cm of the stranger/novel mouse contained in the wired cage ( Leung et al, 2018 ). Sociability was expressed using sociability scores that were defined as the difference between the interaction time with the stranger and empty chambers.…”

Section: Methodsmentioning

confidence: 99%

Effects of glycogen synthase kinase-3β activity inhibition on cognitive, behavioral, and hippocampal ultrastructural deficits in adulthood associated with adolescent methamphetamine exposure

Peng

Liu²,

Ma³

et al. 2023

Front. Mol. Neurosci.

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ObjectiveGlycogen synthase kinase-3β (GSK3β) has been implicated in the maintenance of synaptic plasticity, memory process, and psychostimulant-induced behavioral effects. Hyperactive GSK3β in the Cornu Ammonis 1 (CA1) subregion of the dorsal hippocampus (DHP) was associated with adolescent methamphetamine (METH) exposure-induced behavioral and cognitive deficits in adulthood. This study aimed to evaluate the possible therapeutic effects of GSK3β inhibition in adulthood on adolescent METH exposure-induced long-term neurobiological deficits.MethodsAdolescent male mice were treated with METH from postnatal day (PND) 45–51. In adulthood, three intervention protocols (acute lithium chloride systemic administration, chronic lithium chloride systemic administration, and chronic SB216763 administration within CA1) were used for GSK3β activity inhibition. The effect of GSK3β intervention on cognition, behavior, and GSK3β activity and synaptic ultrastructure in the DHP CA1 subregion were detected in adulthood.ResultsIn adulthood, all three interventions reduced adolescent METH exposure-induced hyperactivity (PND97), while only chronic systemic and chronic within CA1 administration ameliorated the induced impairments in spatial (PND99), social (PND101) and object (PND103) recognition memory. In addition, although three interventions reversed the aberrant GSK3β activity in the DHP CA1 subregion (PND104), only chronic systemic and chronic within CA1 administration rescued adolescent METH exposure-induced synaptic ultrastructure changes in the DHP CA1 subregion (PND104) in adulthood.ConclusionRescuing synaptic ultrastructural abnormalities in the dHIP CA1 subregion by chronic administration of a GSK3β inhibitor may be a suitable therapeutic strategy for the treatment of behavioral and cognitive deficits in adulthood associated with adolescent METH abuse.

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“…As a classic experiment in animal behavior research, the three‐chamber social approach is widely used to determine the sociability of wild animals (Leung et al, 2018; Teyssier et al, 2014) (Figure 1). A test arena is divided into three chambers of social, non‐social, and neutral compartments.…”

Section: Methods Of Experimentationmentioning

confidence: 99%

A review to the methods of personality measurements in wild animals

Cheng,

He,

Zhang

et al. 2023

Wildlife Letters

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One of the challenges in the study of animal personalities is to select a suitable method to measure personality. Here, we reviewed the three common methods (traits rating, behavioral coding, and experimentation) used in animal personality measurement in wild animals. For each method, we summarized its experimental animals and conditions, advantages, and disadvantages in personality measurement. One method could be used to measure the personalities of different animal species, and different methods could be used to measure the personality of one animal species. Overall, traits rating was used to test the personalities of large animals (e.g., primates), behavioral coding was conducted both in large and small animals (e.g., primates, birds, rodents, and fishes), whereas experimentation was used in small animals (e.g., rodents, birds, amphibians, and insects). Although the methods of experimentation were originally designed for model animals (e.g., mice and rats), some of them were used to test the personalities of wild animals under indoor conditions, in which the experimental animals were transferred from the field to the laboratory. Unfortunately, few studies have estimated the applicability of these methods to wild animals. In the future, effective methods of personality measurement are needed for wild animals, especially the methods that can be used in the field.

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Three-chamber Social Approach Task with Optogenetic Stimulation (Mice)

Cited by 7 publications

References 10 publications

Decreased miR-451a in cerebrospinal fluid, a marker for both cognitive impairment and depressive symptoms in Alzheimer's disease

Decreased miR-451a in cerebrospinal fluid, a marker for both cognitive impairment and depressive symptoms in Alzheimer's disease

Effects of glycogen synthase kinase-3β activity inhibition on cognitive, behavioral, and hippocampal ultrastructural deficits in adulthood associated with adolescent methamphetamine exposure

A review to the methods of personality measurements in wild animals

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