Three cDNA clones encoding mouse transplantation antigens: Homology to immunoglobulin genes

Steinmetz, Michael; Frelinger, John G.; Fisher, Douglas A.; Hunkapiller, Tim; Pereira, Dennis; Weissman, Sherman M.; Uehara, Hiroshi; Nathenson, Stanley G.; Hood, Leroy

doi:10.1016/0092-8674(81)90508-0

Cited by 275 publications

(99 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NC2 regions of pH-2 -l and -3 contain a sequence highly repeated in the (8,9). A short stretch found in the Bl sequence (21), namely AATCCCAGC, lies at about 50 nucleotides downstream the border between NCl and NC2.…”

Section: Lye4mentioning

confidence: 99%

“…In contrast, the H-2 like antigens are poorly polymorphic (4). In the course of experiments aimed at understanding the origin of H-2 polymorphism, we (6,7) and others (8) have prepared and characterized cDNA clones copied from gene transcripts of the H-2 multigene family (8,9). We have reported earlier the nucleotide sequences of the coding region of two such clones (7).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family

et al. 1982

View full text Add to dashboard Cite

The complete nucleotide sequences of three cDNAs coding for the C-terminal part of mouse histocompatibility (H-2) antigens, and for the 3' non coding regions of these clones have been determined. Comparison of the sequences indicates a large homology throughout the coding and non-coding regions and suggests the existence of a genetic mechanism which homogenizes nucleotide sequences among genes of the H-2 multigene family.

show abstract

Section: Lye4mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family

et al. 1982

View full text Add to dashboard Cite

show abstract

“…The direct correlation ofa DNA sequence with a specific antigen circumvents those difficulties that arise in subsequent analyses as a result of the numbers of homologous sequences detected by nucleic acid hybridization. For example, hybridization with HLA-A,B or H-2K,D probes detects 10-15 fragments in genomic DNA digests (43)(44)(45). To determine what these fragments are one must resort either to a detailed analysis of each sequence or to cell transformation, which will ultimately depend upon serological methods for detection of transformants and a knowledge ofthe requirements for cell surface expression as well.…”

Section: Resultsmentioning

confidence: 99%

cDNA clones for the heavy chain of HLA-DR antigens obtained after immunopurification of polysomes by monoclonal antibody.

Korman¹,

Knudsen²,

Kaufman³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A monoclonal antibody (HC 2. 1) directed against the separated heavy chain of HLA-DR has been prepared. By binding HC 2.1 to polysomes from human B lymphoblastoid cells followed by the use of a protein A-Sepharose column as an immunoadsorbent, we have purified the mRNA coding for the HLA-DR heavy chain nearly to homogeneity. The immunopurified mRNA has been used to prepare labeled cDNA with which to probe cDNA libraries. Double-stranded cDNA was also made from the immunopurified mRNA and cloned directly into pBR322. Two clones, one from each of the above procedures, positively selected DR heavy chain message as assayed by cell-free translation and immunoprecipitation. One clone, pDRH-2 [500 base pairs plus 75 base pairs of poly(A)] contains the entire 3' untranslated region as well as coding information for the carboxy-terminal hydrophilic intracellular domain and part of the hydrophobic transmembrane region. Results of carboxypeptidase digestion of the heavy chains from detergent-solubilized (p34) and papaintreated (p33) HLA-DR antigen were consistent with the predicted protein sequence. Specific immunopurification of polysomes by defined monoclonal antibodies followed by direct cloning ofcDNA to the highly purified mRNA is a powerful method for obtaining identified cDNA clones.

show abstract

“…Northern blotting and hybridization were carried out as described previously (18). Hybridization probes used were H-2 IIa(sub), a broadly cross-reactive cDNA subclone of H-2 IIa lacking the repetitive sequences (19,20); ATF-1, excised by BamHI and HindIII from the ATF-1A clone (see Fig. 1); and ␤-actin (21).…”

Section: Methodsmentioning

confidence: 99%

Activation Transcription Factor 1 Involvement in the Regulation of Murine H-2D Expression

Ishiguro

Brown

Meruelo

1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

Resistance to radiation leukemia virus-induced leukemia is correlated with an increase in H-2D expression on the thymocyte surface. Recently, it has been shown that elevated H-2D d expression on the infected thymocyte is a result of elevated mRNA transcription and that the transcriptional increase is correlated with elevated levels of a DNA binding activity, H-2 binding factor 1 (H-2 BF1), which recognizes the 5 -flanking sequences (5 -TGACGCG-3 ) of the H-2D d gene. This target for transcription factor binding has been found to be identical in the 5 -regulatory region of 12 rodent class I genes, nine of which have been shown to be functional genes. Furthermore, this cis-element is found 5 of 20 primate class I genes (15 human genes), seven of which are known to be functional. Here, we demonstrate that activation transcription factor 1 (ATF-1) is one component of H-2 BF1. In addition, the levels of ATF-1 mRNA in uninfected and radiation leukemia virus-infected thymocytes parallel those of H-2D d mRNA, and therefore, it is suggested that ATF-1 up-regulates the transcription of the H-2D d gene after radiation leukemia virus infection of thymocytes. Transfection experiments also demonstrate that ATF-1 activates a reporter plasmid that contains the H-2 BF1 motif, but not a reporter lacking this motif. This is the first demonstration of the interaction of ATF-1 with 5 -regulatory sequences of major histocompatibility complex class I genes.

show abstract

Three cDNA clones encoding mouse transplantation antigens: Homology to immunoglobulin genes

Cited by 275 publications

References 44 publications

Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family

Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family

cDNA clones for the heavy chain of HLA-DR antigens obtained after immunopurification of polysomes by monoclonal antibody.

Activation Transcription Factor 1 Involvement in the Regulation of Murine H-2D Expression

Contact Info

Product

Resources

About