Three ABTS•+ radical cation-based approaches for the evaluation of antioxidant activity: fast- and slow-reacting antioxidant behavior

Ilyasov, Igor R.; Beloborodov, V. L.; Селиванова, И. А.

doi:10.1007/s11696-018-0415-9

Cited by 43 publications

(34 citation statements)

References 25 publications

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“…The stoichiometry of the process is another serious concern. The glutathione (GSH) was found to give higher scavenging stoichiometry with ABTS •+ than the expected 1-eoxidation, which was possibly due to the deeper GSH oxidation to sulphenic and sulphinic acids rather than only to glutathione disulfide glutathione disulfide (GSSG) [58,66]. This was evidenced by the fact that oxidized glutathione GSSG also showed the ability to slowly decolorize ABTS •+ , apparently due to the further oxidation of sulfur [60,67,68].…”

Section: Reaction Stoichiometrymentioning

confidence: 98%

“…One of the most serious shortcomings of ABTS-based methods is that, even for some standard antioxidants such as quercetin, naringin, or glutathione, different TEAC values can be observed between laboratories. The main two reasons are the dependence of the relative antioxidant capacity (e.g., TEAC index) on the concentration for some antioxidants [52,[57][58][59] and the incompleteness of ABTS •+ scavenging reaction by 6th and even more so by the 4th minute; moreover, by the 30th min some antioxidants still demonstrate gradual ABTS •+ inhibition [56,[60][61][62][63][64][65].…”

Section: Reaction Stoichiometrymentioning

confidence: 99%

“…It is likely that this excess of ABTS •+ , as well as the relatively high rates of ABTS •+ consumption in sideline reactions, are among the reasons for the high stoichiometry. For example, the same ABTS/PP system gave much more adequate stoichiometric coefficients when ABTS •+ was generated in situ in the presence of antioxidants in a lag-time assay [58]. In this variant, the solution simultaneously contained antioxidant, potassium persulfate, and the excess of reduced/unreacted ABTS.…”

Section: Taxifolinmentioning

confidence: 99%

“…At the same time, naringenin and apigenin did not give any classical initial lag-time at all-ABTS •+ accumulation in their presence showed different kinetics, demonstrating no initial lag-time, but a kind of delayed lag-time. Finally, morin scavenged ABTS •+ in an easily distinguishable two-stage manner, giving both the initial and delayed lag-time [58].…”

Section: Taxifolinmentioning

confidence: 99%

“…The calculated data from[58], considering ABTS •+ radical cation λmax 734, ε 15,000 L mol −1 cm −1 , and antioxidant concentrations that cause 50% inhibition or 15 min lag-time of the ABTS •+ .…”

mentioning

confidence: 99%

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ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways

Ilyasov

Beloborodov

Селиванова

et al. 2020

IJMS

Self Cite

286

161

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The 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ) radical cation-based assays are among the most abundant antioxidant capacity assays, together with the 2,2-diphenyl-1picrylhydrazyl (DPPH) radical-based assays according to the Scopus citation rates. The main objective of this review was to elucidate the reaction pathways that underlie the ABTS/potassium persulfate decolorization assay of antioxidant capacity. Comparative analysis of the literature data showed that there are two principal reaction pathways. Some antioxidants, at least of phenolic nature, can form coupling adducts with ABTS •+ , whereas others can undergo oxidation without coupling, thus the coupling is a specific reaction for certain antioxidants. These coupling adducts can undergo further oxidative degradation, leading to hydrazindyilidene-like and/or imine-like adducts with 3-ethyl-2-oxo-1,3-benzothiazoline-6-sulfonate and 3-ethyl-2-imino-1,3-benzothiazoline-6-sulfonate as marker compounds, respectively. The extent to which the coupling reaction contributes to the total antioxidant capacity, as well as the specificity and relevance of oxidation products, requires further in-depth elucidation. Undoubtedly, there are questions as to the overall application of this assay and this review adds to them, as specific reactions such as coupling might bias a comparison between antioxidants. Nevertheless, ABTS-based assays can still be recommended with certain reservations, particularly for tracking changes in the same antioxidant system during storage and processing.

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Section: Reaction Stoichiometrymentioning

confidence: 98%

Section: Reaction Stoichiometrymentioning

confidence: 99%

Section: Taxifolinmentioning

confidence: 99%

Section: Taxifolinmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways

Ilyasov

Beloborodov

Селиванова

et al. 2020

IJMS

Self Cite

286

161

View full text Add to dashboard Cite

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Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

Guo

et al. 2020

Chemistry & Biodiversity

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Medical fungi polysaccharides belong to a very important species of biological macromolecules, which are the basic substances that effectively maintain and ensure the normal operation of biological life activities. However, research on extraction and biological activity of Inonotus cuticularis polysaccharides has never been reported. In this study, the optimum yield of Inonotus cuticularis polysaccharides was determined by the orthogonal experimental design. The highest yield of 3.10±0.06 % was obtained with extraction temperature of 80 °C, extraction time of 150 min, and water to raw material ratio of 30 mL/g and repeated twice. After deproteinization for 5 times, the protein removal rate reached 70.10±1.75 %, and the content of polysaccharides and protein were 46.64 and 0.42 %. Infrared spectrometer indicated that Inonotus cuticularis polysaccharides are typical β‐pyranose with characteristic peaks of polysaccharides. Subsequently, the activities of scavenging free radicals for the deproteinated polysaccharides were studied. When the concentration of Inonotus cuticularis polysaccharides was 0.3 mg/mL, the scavenging activities of the sample on DPPH., .OH, ABTS.+ and O2.− reached 83.67±0.27, 65.21±4.82, 43.45±1.36 and 80.28±2.30 %, respectively, and the reducing power reached 0.46±0.01. The IC50 values scavenging DPPH., .OH, ABTS.+ and O2.− were 0.139±0.13, 0.162±0.14, 0.317±0.30 and 0.121±0.10 mg/mL, respectively. Results showed that Inonotus cuticularis polysaccharides present potential stronger antioxidant activities, especially .OH scavenging activity and reducing power. Experimental results could provide research basis of Inonotus cuticularis polysaccharides for further exploitation and utilization.

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Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas

Tewari,

Bawari,

Mishra

et al. 2024

J of Separation Science

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Swertia species are common ingredients in numerous herbal remedies. It is also used to treat a wide range of illnesses and possess diverse therapeutic activities. The aim of the study is to elucidate the comprehensive metabolomics profile of Swertia chirayita and the role of various extraction methods in the phytochemical compositions of the extracts of S. chirayita, and their antioxidant and enzyme inhibitory activities. Extraction of the stems, leaves, and flowering tops of S. chirayita was performed by maceration, infusion, and soxhlation using methanol and water as solvent. Extracts were subjected to phytochemical profiling by a liquid‐chromatographic system. Antioxidant and enzyme inhibitory activity was carried out. The metabolomics profiling showed that a diverse range of specialized metabolites were present in the stems and leaves & flowering tops of the plant. All the extracts showed substantial antioxidant and enzyme inhibitory activities further confirmed by molecular docking studies. This study appraised the use of S. chirayita aerial parts as a potential antioxidant and its therapeutic application in various chronic illnesses including Alzheimer's disease, diabetes, and other skin‐related disorders.

show abstract

Three ABTS•+ radical cation-based approaches for the evaluation of antioxidant activity: fast- and slow-reacting antioxidant behavior

Cited by 43 publications

References 25 publications

ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways

ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways

Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas

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