Threatened Woody Plants of Georgia and Micropropagation as a Tool for In Vitro Conservation

Gaidamashvili, Mariam; Benelli̇, C.

doi:10.3390/agronomy11061082

Cited by 11 publications

(2 citation statements)

References 95 publications

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“…Biotechnological methods may be valuable in the propagation and conservation of selected genotypes (Gaidamashvili and Benelli, 2021). In vitro propagation is not only less affected by the external environment but can also produce orderly and consistent young plants with ideal characteristics (Kumar et al, 2011;Martínez et al, 2021;Chakraborty et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

Zhang

Guo²,

Wu³

et al. 2022

Front. Plant Sci.

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Castanopsis hystrix is one of the main timber trees grown in China. However, severe shortage of natural seeds and the difficulty of explant regeneration has limited seedling supply. As such, there is a need for research on asexual multiplication of C. hystrix. This study established a rapid propagation technology system for C. hystrix genotypes, including explant treatment, proliferation, and rooting. HZ (a modified MS medium) supplemented with 4.4 μM BA and 0.5 μM IBA was found to be the optimal medium for shoot sprouting. The maximum proliferation coefficient and the number of effective shoots was obtained on HZ medium supplemented with 2.6 μM BA and 1.0 μM IBA, were 3.00 and 5.63, respectively. A rooting rate of 83.33% was achieved using half-strength HZ medium supplemented with 3.2 μM NAA. Adding vitamin C (80 mg⋅l–1) for 7 days in a dark environment reduced the browning rate, while increasing the proliferation rate. Additionally, through cytological observation, we established how and where adventitious roots occur. The survival rate of transplanted plantlets was > 90%. This is the first report of an in vitro regeneration technique that uses stem segments of mature C. hystrix as explants.

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Section: Introductionmentioning

confidence: 99%

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

Zhang

Guo²,

Wu³

et al. 2022

Front. Plant Sci.

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“…The use of biotechnological approaches may be a valuable option regarding the propagation and conservation of selected genotypes [19], with axillary shoot proliferation being the most widely used method for vegetative propagation [20]. Unfortunately, in holm oak, only one report deals on micropropagation using explants derived from mature trees [21].…”

Section: Introductionmentioning

confidence: 99%

Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

Martínez

Arrillaga

Sales

et al. 2021

Forests

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Holm oak populations have deteriorated drastically due to oak decline syndrome. The first objective of the present study was to investigate the use of axillary budding and somatic embryogenesis (SE) to propagate asymptomatic holm oak genotypes identified in disease hotspots in Spain. Axillary budding was achieved in two out of six tolerant genotypes from the south-western region and in two out of four genotypes from the Mediterranean region. Rooting of shoots cultured on medium supplemented with 3 mg L−1 of indole-3-acetic acid plus 0.1 mg L−1 α-naphthalene acetic acid was achieved, with rates ranging from 8 to 36%. Shoot cultures remained viable after cold storage for 9–12 months; this procedure is therefore suitable for medium-term conservation of holm oak germplasm. SE was induced in two out of the three genotypes tested, by using nodes and shoot tips cultured in medium without plant growth regulators. In vitro cloned progenies of the tolerant genotypes PL-T2 and VA5 inhibited growth of Phytophthora cinnamomi mycelia when exposed to the oomycete in vitro. Significant differences in total phenol contents and in the expression profiles of genes regulating phenylpropanoid biosynthesis were observed between in vitro cultured shoots derived from tolerant trees and cultures established from control genotypes.

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Long- and Medium-Term Storage of Germplasm for Conservation of Tree Species

Yadav,

Priya,

Dhiman

et al. 2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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Threatened Woody Plants of Georgia and Micropropagation as a Tool for In Vitro Conservation

Cited by 11 publications

References 95 publications

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

Long- and Medium-Term Storage of Germplasm for Conservation of Tree Species

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