THP9 enhances seed protein content and nitrogen-use efficiency in maize

Huang, Yongcai; Wang, Haihai; Zhu, Yidong; Huang, Xing; Li, Shuai; Wu, Xingguo; Zhao, Yao; Bao, Zhigui; Qin, Li; Jin, Yongbo; Cui, Yahui; Ma, Guangjin; Xiao, Qiao; Wang, Qiong; Wang, Jiechen; Yang, Xuerong; Liu, Hongjun; Lu, Xiaoduo; Wang, Wenqin; Wu, Yongrui

doi:10.1038/s41586-022-05441-2

Cited by 69 publications

(35 citation statements)

References 72 publications

(79 reference statements)

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“…These findings suggest new varieties with high GPC can be developed by pyramiding these superior alleles. While previous studies have suggested a negative correlation between grain yield and GPC in cereal crops [ 33 , 38 ], some QTLs for GPC have been found to have no negative effect on grain yield [ 37 , 39 , 40 ], indicating the potential for improving GPC without compromising yield by incorporating these QTLs into new varieties. However, as the grain yield of the 174 oat accessions in this study was not investigated, the effects of the identified GPC QTLs on grain yield remain unknown.…”

Section: Discussionmentioning

confidence: 98%

Genome-Wide Association Mapping of QTL Underlying Groat Protein Content of a Diverse Panel of Oat Accessions

Yan

Zhang

Zhou

et al. 2023

IJMS

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Groat protein content (GPC) is a key quality trait attribute in oat. Understanding the variation of GPC in oat germplasms and identifying genomic regions associated with GPC are essential for improving this trait. In this study, the GPC of 174 diverse oat accessions was evaluated in three field trials. The results showed a wide variation in GPC, ranging from 6.97% to 22.24% in this panel. Hulless oats displayed a significantly higher GPC compared to hulled oats across all environments. A GWAS analysis was performed based on 38,313 high-quality SNPs, which detected 27 non-redundant QTLs with 41 SNPs significantly associated with GPC. Two QTLs on chromosome 6C (QTL16) and 4D (QTL11) were consistently detected in multiple environments, with QTL16 being the most significant and explaining the highest proportion of the phenotypical variation in all tested environments except in CZ20. Haplotype analysis showed that the favorable haplotypes for GPC are more prevalent in hulless oats. These findings provide a foundation for future efforts to incorporate favorable alleles into new cultivars through introgression, fine mapping, and cloning of promising QTLs.

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Section: Discussionmentioning

confidence: 98%

Genome-Wide Association Mapping of QTL Underlying Groat Protein Content of a Diverse Panel of Oat Accessions

Yan

Zhang

Zhou

et al. 2023

IJMS

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“…In an earlier study, researchers have found that a 191-bp insertion in the intron affected the expression levels of the polyphenol oxidase gene, resulting in a lower PPO activity in wheat [ 35 ]. Recent research has also reported that THP9 encoded an asparagine synthetase 4 enzyme which was highly expressed in teosinte, but not in the B73 inbred, in which a deletion in the tenth intron of THP9-B73 caused incorrect splicing of THP9-B73 transcripts [ 36 ]. In this paper, we also induced a 36-bp deletion (Del36) in the fifth intron of G-GmPAP14Z .…”

Section: Discussionmentioning

confidence: 99%

Allelic Variation in GmPAP14 Alters Gene Expression to Affect Acid Phosphatase Activity in Soybean

Kong

Liu

et al. 2023

IJMS

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Improvement in acid phosphatase (APase) activity is considered as an important approach to enhance phosphorus (P) utilization in crops. Here, GmPAP14 was significantly induced by low P (LP), and its transcription level in ZH15 (P efficient soybean) was higher than in NMH (P inefficient soybean) under LP conditions. Further analyses demonstrated that there were several variations in gDNA (G-GmPAP14Z and G-GmPAP14N) and the promoters (P-GmPAP14Z and P-GmPAP14N) of GmPAP14, which might bring about differential transcriptional levels of GmPAP14 in ZH15 and NMH. Histochemical staining measurements revealed that a stronger GUS signal was present in transgenic Arabidopsis with P-GmPAP14Z under LP and normal P (NP) conditions compared with the P-GmPAP14N plant. Functional research demonstrated that transgenic Arabidopsis with G-GmPAP14Z had a higher level of GmPAP14 expression than the G-GmPAP14N plant. Meanwhile, higher APase activity was also observed in the G-GmPAP14Z plant, which led to increases in shoot weight and P content. Additionally, validation of variation in 68 soybean accessions showed that varieties with Del36 displayed higher APase activities than the del36 plant. Thus, these results uncovered that allelic variation in GmPAP14 predominantly altered gene expression to influence APase activity, which provided a possible direction for research of this gene in plants.

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“…The starch content of the mature seeds was determined as has been previously described by [ 60 ]. For the protein quantification, 50 mg of powder was used for the protein extraction according to the method described by [ 61 ]. Then, the protein content was measured using a Bradford Protein Assay Kit (PC0010, Solarbio, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

Wang

et al. 2023

Plants

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The kernel serves as a storage organ for various nutrients and determines the yield and quality of maize. Understanding the mechanisms regulating kernel development is important for maize production. In this study, a small-kernel mutant smk7a of maize was characterized. Cytological observation suggested that the development of the endosperm and embryo was arrested in smk7a in the early development stage. Biochemical tests revealed that the starch, zein protein, and indole-3-acetic acid (IAA) contents were significantly lower in smk7a compared with wild-type (WT). Consistent with the defective development phenotype, transcriptome analysis of the kernels 12 and 20 days after pollination (DAP) revealed that the starch, zein, and auxin biosynthesis-related genes were dramatically downregulated in smk7a. Genetic mapping indicated that the mutant was controlled by a recessive gene located on chromosome 2. Our results suggest that disrupted nutrition accumulation and auxin synthesis cause the defective endosperm and embryo development of smk7a.

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THP9 enhances seed protein content and nitrogen-use efficiency in maize

Cited by 69 publications

References 72 publications

Genome-Wide Association Mapping of QTL Underlying Groat Protein Content of a Diverse Panel of Oat Accessions

Genome-Wide Association Mapping of QTL Underlying Groat Protein Content of a Diverse Panel of Oat Accessions

Allelic Variation in GmPAP14 Alters Gene Expression to Affect Acid Phosphatase Activity in Soybean

Characterization and Transcriptome Analysis of Maize Small-Kernel Mutant smk7a in Different Development Stages

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