THP1 macrophages oxidized cholesterol, generating 7-derivative oxysterols specifically released by HDL

Chen, Yinan; Arnal-Levron, Maud; Lagarde, Michel; Moulin, Philippe; Luquain-Costaz, Céline; Delton, Isabelle

doi:10.1016/j.steroids.2015.02.020

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…THP-1 human monocytic cells (ATCC TIB-202) were cultured in RPMI 1640 supplemented with 10% FBS, 100U/ml penicillin and 100µg/ml streptomycin in a 5% CO2-humidified incubator at 37°C. They were differentiated into macrophages by exposure to 50ng/ml phorbol 12-myristate 13-acetate (PMA) for 72h [43,44].…”

Section: Cell Culturementioning

confidence: 99%

Bis(monoacylglycero)phosphate, a new lipid signature of endosome-derived extracellular vesicles

et al. 2020

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Section: Cell Culturementioning

confidence: 99%

Bis(monoacylglycero)phosphate, a new lipid signature of endosome-derived extracellular vesicles

et al. 2020

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“…Chadjichristos CE found that Cx40 promotes leukocyte adhesion to the endothelium and accelerates atherosclerosis (Chadjichristos et al, 2010). GO: 0006979, representing response to oxidative stress, also participates in atherosclerosis.Chen Y et al revealed that fatty acid metabolism is disordered in response to oxidative stress from the mitochondria, which drives chronic inflammation and accelerates the process of atherosclerosis (Chen et al, 2015). Therefore, we speculated that the DE lncRNAs might regulate the progression of LAA stroke by targeting genes.…”

Section: Discussionmentioning

confidence: 89%

Novel Insights into the Potential Diagnostic Value of Circulating Exosomal IncRNA-Related Networks in Large Artery Atherosclerotic Stroke

Zhang

Wang

et al. 2021

Front. Mol. Biosci.

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Exosomes show diagnostic and therapeutic promise as carriers of ncRNAs in diseases. LncRNAs in exosomes have been identified as being stable and avoided degradation by nucleolytic enzymes. Although lncRNAs have been confirmed to be important in cancers, no studies for exo-lncRNAs have been reported in LAA stroke. High-throughput sequencing was performed to detect the differential expression profiles of lncRNAs in five paired plasma-derived exosome samples from patients with LAA stroke and controls (matched on vascular risk factors). Exo-lncRNA-associated networks were predicted with a combination of multiple databases. The expression of the selected genes in the networks was confirmed by qRT-PCR in a validation set (LAA vs. controls = 30:30). Furthermore, ROC analysis was used to evaluate the diagnostic performance of the lncRNA-related networks. A total of 1,020 differentially expressed lncRNAs were identified in LAA stroke patients. GO and KEGG pathway analyses indicated that their target genes are involved in atherosclerosis-related pathways, including inflammation, cell adhesion, and cell migration. qRT-PCR confirmed that the expression trend of differential expressed genes was consistent with RNA-seq. Furthermore, the AUCs of the lnc_002015-related network and lnc_001350-related network were 0.959 and 0.97, respectively, in LAA stroke. Our study showed the differential expression of lncRNAs in plasma exosomes and presented related diagnostic networks for LAA stroke for the first time. The results suggested that exosomal lncRNA-related networks could be potential diagnostic tools in LAA stroke.

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“…CE is fairly inert and eventually stored in lipid droplet. During atherogenesis in the presence of some particular factors such as macrophage LPL, oxLDLs may become highly atherogenic as they could induce a significant cholesterol accumulation in subendothelial macrophages, leading to the formation of foam cells[ 39 ]. These findings further support that miR-590 may act as a protective factor on atherosclerotic cardiovascular disease.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-590 Inhibits Lipoprotein Lipase Expression and Prevents Atherosclerosis in apoE Knockout Mice

Ouyang

et al. 2015

PLoS ONE

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Recent studies have suggested that miR-590 may play critical roles in cardiovascular disease. This study was designed to determine the effects of miR-590 on lipoprotein lipase (LPL) expression and development of atherosclerosis in apolipoprotein E knockout (apoE−/−) mice and explore the potential mechanisms. En face analysis of the whole aorta revealed that miR-590 significantly decreased aortic atherosclerotic plaque size and lipid content in apoE−/− mice. Double immunofluorescence staining in cross-sections of the proximal aorta showed that miR-590 agomir reduced CD68 and LPL expression in macrophages in atherosclerotic lesions. MiR-590 agomir down-regulated LPL mRNA and protein expression as analyzed by RT-qPCR and western blotting analyses, respectively. Consistently, miR-590 decreased the expression of CD36 and scavenger receptor A1 (SRA1) mRNA and protein. High-performance liquid chromatography (HPLC)analysis confirmed that treatment with miR-590 agomir reduced lipid levels either in plasma orinabdominal cavity macrophages of apoE−/− mice. ELISA analysis showed that miR-590 agomir decreased plasma levels of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein-1 (MCP-1), interleukin-1β (IL-1β)and interleukin-6 (IL-6). In contrast, treatment with miR-590 antagomir prevented or reversed these effects. Taken together, these results reveal a novel mechanism of miR-590 effects, and may provide new insights into the development of strategies for attenuating lipid accumulation and pro-inflammatory cytokine secretion.

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THP1 macrophages oxidized cholesterol, generating 7-derivative oxysterols specifically released by HDL

Cited by 11 publications

References 26 publications

Bis(monoacylglycero)phosphate, a new lipid signature of endosome-derived extracellular vesicles

Bis(monoacylglycero)phosphate, a new lipid signature of endosome-derived extracellular vesicles

Novel Insights into the Potential Diagnostic Value of Circulating Exosomal IncRNA-Related Networks in Large Artery Atherosclerotic Stroke

MicroRNA-590 Inhibits Lipoprotein Lipase Expression and Prevents Atherosclerosis in apoE Knockout Mice

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