Thoracic Ultrasound for Diagnosing Pulmonary Embolism

Mathis, Gebhard; Blank, Wolfgang; Reißig, Angelika; Lechleitner, Peter; Reuß, Joachim; Schüler, Andreas; Beckh, Sonja

doi:10.1378/chest.128.3.1531

Cited by 256 publications

(85 citation statements)

References 26 publications

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“…Pleural effusion CTLs served as surrogates for lung TILs since it has been reported that these CTLs have experienced a similar microenvironment as that of tumors and display similar phenotypes as those of TILs. [27][28] Indeed, our functional analysis showed that, in comparison to CTLs collected from PBMCs, CTLs from the pleural effusion of NSCLC cancer patients exhibited a deficiency in granzyme B production (Fig. S1).…”

Section: Resultsmentioning

confidence: 93%

“…6A and B). Consequently, based on in vitro cytotoxicity assays with gp100 [25][26][27][28][29][30][31][32][33] peptidepulsed EL4 lymphoma cells, the tumor-specific killing capacity of miR-26a Decoy pMel-1 cells was significantly improved (Fig. 6C).…”

Section: Mir-26a Decoy Is Sufficient To Rescue Tme-suppressed Ctl Funmentioning

confidence: 99%

“…3A) and reported to be involved in CTL function. 27,28 We took a targeting decoy approach 15 to validate the impact of miR-26a on candidate gene expression. A miR-26a decoy was designed as a tandem of eight different miR-26a binding elements attached to the 3-end of a GFP gene.…”

Section: Ezh2 Is a Bona Fide Target Of Mir-26amentioning

confidence: 99%

“…pMel-1 transgenic CD8 C T cells were primed in vitro with their cognate antigen (gp100 [25][26][27][28][29][30][31][32][33] peptide) and transduced with retrovirus expressing mock or miR-26a Decoy . Two days after initial activation, CD8 C T cells were deprived of antigen, or treated with CM (B16 cells derived) for an additional 48 h. As expected, even under optimal priming conditions, miR-26a Decoy was capable of enhancing IFNg and granzyme B protein production by CTLs, based on both population percentage and per-cell production.…”

Section: Mir-26a Decoy Is Sufficient To Rescue Tme-suppressed Ctl Funmentioning

confidence: 99%

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The tumor microenvironment disarms CD8⁺ T lymphocyte function via a miR-26a-EZH2 axis

Long

Xiang

et al. 2016

OncoImmunology

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One of the most important factors that limit the potency of CD8 C cytotoxic T lymphocyte (CTL) responses is the tumor microenvironment (TME). Here, we provide evidence that miR-26a is a negative regulator of CTL function in the TME. Specifically, we identified miR-26a as a crucial suppressor gene in CTLs from the TME, as we found that, miR-26a expression was elevated in CTLs to respond to TME secretome stimulation. CTLs from miR-26a-transgenic mice showed impaired IFNg and granzyme B production in response to their cognate antigen. Conversely, we found that miR-26a inhibition in CTLs could effectively increase the cytotoxicity and suppress tumor growth. Mechanically, we identified EZH2 as a direct target of miR-26a. miR-26a and EZH2 expression were found to be inversely correlated in CTLs, and the inhibition of EZH2 in CTLs impairs CTL function. These functional correlations were validated in a cohort of non-small cell lung cancer patients, indicating that the miR-26a-EZH2 axis is clinically relevant. Our findings suggested that miR-26a silencing as a novel strategy to improve the efficacy of CTL-based cancer immunotherapy.

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Section: Resultsmentioning

confidence: 93%

Section: Mir-26a Decoy Is Sufficient To Rescue Tme-suppressed Ctl Funmentioning

confidence: 99%

Section: Ezh2 Is a Bona Fide Target Of Mir-26amentioning

confidence: 99%

Section: Mir-26a Decoy Is Sufficient To Rescue Tme-suppressed Ctl Funmentioning

confidence: 99%

See 2 more Smart Citations

The tumor microenvironment disarms CD8⁺ T lymphocyte function via a miR-26a-EZH2 axis

Long

Xiang

et al. 2016

OncoImmunology

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“…Researchers have examined patterns of B lines in ALI/ARDS 19 , 21 – 23 and identified pleural line abnormalities in pleuritis, 24 pneumonia 24 – 28 and pulmonary emboli 29 . These signs reduced the false positive rate among the more sick patients although some were present in the true positives.…”

Section: Recognition Of Patients With Other Lung Pathologymentioning

confidence: 99%

Lung ultrasound in heart failure: Lessons from re‐analysis of Lung Ultrasound 2011 database

Baker

Mitchell

Thompson

et al. 2015

Australas J Ultrason Med

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Introduction: In the setting of patients presenting with shortness of breath to an Emergency Department a simple lung ultrasound protocol aimed at detecting pulmonary oedema has been shown to have diagnostic accuracy of 85%. This article reviews data from the original study, in an attempt to determine whether adjusting the protocol and/or interpretive criteria would improve results. Method: A large lung ultrasound project provided the dataset. Inter‐rater and intertest discrepancies were reviewed. Then original stored images and comments were retrospectively analysed using alternate interpretive criteria. Specific variations included changing the number of B‐lines required to define ‘wet lung’ and assessing other pleural line abnormalities. Where they had been acquired cardiac loops were reviewed in addition to the lung images. Results: The 204 original studies available were reviewed. Some disagreement could be attributed to inexperience and unclear definitions. Adjusting the number of B‐lines did not improve diagnostic accuracy. All positive scans, with numerous B‐lines were reviewed using more advanced diagnostic criteria (pleural line abnormalities) and the number of false positives was decreased. In cases where cardiac views were available, their inclusion was beneficial. Conclusion: A simple lung ultrasound protocol to assess for ‘wet lung’ in patients presenting to Emergency Departments provides diagnostic accuracy of around 85% in the hands of relative novices. More advanced interpretation of the same_ultrasound images, and the addition of cardiac views, is likely to further improve diagnostic accuracy.

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Pulmonary Thromboembolism in Infants and Children

Stein

2007

Pulmonary Embolism

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Thoracic Ultrasound for Diagnosing Pulmonary Embolism

Cited by 256 publications

References 26 publications

The tumor microenvironment disarms CD8⁺ T lymphocyte function via a miR-26a-EZH2 axis

The tumor microenvironment disarms CD8⁺ T lymphocyte function via a miR-26a-EZH2 axis

Lung ultrasound in heart failure: Lessons from re‐analysis of Lung Ultrasound 2011 database

Pulmonary Thromboembolism in Infants and Children

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Thoracic Ultrasound for Diagnosing Pulmonary Embolism

Cited by 256 publications

References 26 publications

The tumor microenvironment disarms CD8+ T lymphocyte function via a miR-26a-EZH2 axis

The tumor microenvironment disarms CD8+ T lymphocyte function via a miR-26a-EZH2 axis

Lung ultrasound in heart failure: Lessons from re‐analysis of Lung Ultrasound 2011 database

Pulmonary Thromboembolism in Infants and Children

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The tumor microenvironment disarms CD8⁺ T lymphocyte function via a miR-26a-EZH2 axis

The tumor microenvironment disarms CD8⁺ T lymphocyte function via a miR-26a-EZH2 axis