Thoracic Duct Lymph of the Calf from Birth to Six Days of Age

Shannon, A. D.; Lascelles, A. K.

doi:10.1038/icb.1968.110

Cited by 2 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, for studies using replacement milk, thoracic duct-venous shunts were established in four bull calves of mixed breeds (Friesian, Guernsey, and Jersey cross breeds). These calves weighed an average of 32·0 kg at the time of operation and their care and management was as previously described (Shannon and Lascelles 1967).…”

Section: (A) Animalsmentioning

confidence: 99%

“…Samples were obtained from calves used in previous studies (Shannon andLascelles 1967, 1968) in which recirculating lymphatico-venous shunts were established between either the thoracic or intestinal lymph ducts and the jugular vein. The calves were Friesians of either sex and surgery was carried out in the first week of life.…”

Section: (A) Animalsmentioning

confidence: 99%

“…), phospholipid, free fatty acid (F.F.A. ), total protein, and albumin in lymph and blood plasma as previously described (Shannon andLascelles 1967, 1968). Total cholesterol in lymph and blood plasma was estimated by the method of Abell et al (1952).…”

Section: (B) Analytical Techniquesmentioning

confidence: 99%

“…Some lipid samples were separated into neutral lipid and phospholipid fractions on small columns of silicic acid (Kernohan, Lascelles, and Wadsworth 1971). Methyl esters of fatty acids were prepared by dimethyl carbonate-induced transesterification and analysed by gas-liquid chromatography as reported by Wadsworth (1968a). Methyl esters prepared in this way from the neutral lipid of lymph and blood plasma represent triglyceride fatty acids since lymph and plasma contain little partial glyceride and dimethyl carbonate-induced transesterification does not esterify cholesterol ester or F.F.A.…”

Section: (B) Analytical Techniquesmentioning

confidence: 99%

“…Previous studies have shown that most of this lipid is absorbed by way of the lymph (Shannon and Lascelles 1967). The opportunity was taken to use samples from these studies to compare the fatty acid composition of lymph lipid with that of the dietary lipid.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of Whole-Milk and Replacement-Milk Feeding on the Fatty Acid Composition of Lymph Lipids in Young Calves

Wadsworth¹,

Shannon²

1971

Aust. Jnl. Of Bio. Sci.

Self Cite

View full text Add to dashboard Cite

Lipids in thoracic duct and intestinal lymph from young calves fed whole milk or replacement milk were analysed. Lymph triglyceride contained Cs and longer fatty acids with an average molecular weight of 260. The major components were 14 : 0, 16 : 0, 18 : 0, and 18 : 1, with small amounts of branched· chain and odd carbonnumber fatty acids. ClO fatty acid from milk lipid was apparently absorbed entirely into lymph. The composition of lymph triglyceride closely reflected that of the diet and showed no diurnal variation when the calves were fed once daily. Triglyceride from blood plasma resembled that from lymph except for higher proportions of 18 : 2 and 18 : 3.Feeding of replacement milk depressed lymph flow to 72% of that during whole·milk feeding and this was apparently due to a decrease in blood flow through the intestinal capillaries. There was also a reduction in the recovery of dietary longchain fatty acid as neutral lipid in lymph during replacement-milk feeding (50% compared with 83%) which could not be accounted for by malabsorption of saturated fatty acids in the replacement milk. These effects were attributed to the presence of a factor in the replacement milk which may have arisen from the oxidation of unsaturated fatty acids.

show abstract

Section: (A) Animalsmentioning

confidence: 99%

Section: (A) Animalsmentioning

confidence: 99%

Section: (B) Analytical Techniquesmentioning

confidence: 99%

Section: (B) Analytical Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Whole-Milk and Replacement-Milk Feeding on the Fatty Acid Composition of Lymph Lipids in Young Calves

Wadsworth¹,

Shannon²

1971

Aust. Jnl. Of Bio. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

Lymph Flow and Protein Composition of Thoracic Duct Lymph in the Newborn Calf

Shannon

Lascelles

1968

Exp Physiol

View full text Add to dashboard Cite

The changes in the flow and protein composition of thoracic duct lymph have been studied in unanesthetized calves during the first 6 days after birth. Thoracic duct-venous shunts were established in four calves (average body weight 35-2 kg.) within 3 hr. of birth. The calves recovered quickly from the operation and were able to suck strongly within 1-2 hr. of its completion. Lymph flow during anmesthesia varied between 90-150 ml./hr. and by 1-2 hr. after the operation, immediately before first feeding, flow had risen to values of 300-450 ml./hr. There was a marked increase in flow and globulin concentration during the 8-hr. period after the first feeding, peak values observed being 850 ml./hr. and 2-72 g. per cent, respectively. Fractionation of colostral whey and lymph on G-200 and DEAE Sephadex columns indicated that the increase in globulin in the lymph was due to the absorption of y,-globulin which is present in high concentration in colostrum. Subsequently, flow and protein content decreased quite rapidly and by 24 hr. after first feeding the values were relatively constant. The results suggested that the absorption of y-globulin had virtually ceased by 24 hr. There was a slower decrease in lymph flow and globulin content over the subsequent 4-6 days.

show abstract

Thoracic Duct Lymph of the Calf from Birth to Six Days of Age

Cited by 2 publications

References 0 publications

Effect of Whole-Milk and Replacement-Milk Feeding on the Fatty Acid Composition of Lymph Lipids in Young Calves

Effect of Whole-Milk and Replacement-Milk Feeding on the Fatty Acid Composition of Lymph Lipids in Young Calves

Lymph Flow and Protein Composition of Thoracic Duct Lymph in the Newborn Calf

Contact Info

Product

Resources

About