THOR’s Hammer: the Antibiotic Koreenceine Drives Gene Expression in a Model Microbial Community

Hurley, Amanda; Chevrette, Marc G.; Rosario-Meléndez, Natalia; Handelsman, Jo

doi:10.1128/mbio.02486-21

Cited by 12 publications

(4 citation statements)

References 69 publications

(76 reference statements)

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“…However, the relative importance of these bacterial metabolites in their interactions with plants is yet to be explored. In addition, Flavobacterium has been selected as a member of the simplified model system involving three bacterial species coisolated from field-grown soybean roots to study community-level interactions [95,96]. Recent investigation showed that the dynamics and functions of this synthetic community may be modulated by and contribute to the regulation of secondary metabolism of the consortium members including Flavobacterium [97].…”

Section: Association With Host Health and Diseasementioning

confidence: 99%

Importance of Bacteroidetes in host–microbe interactions and ecosystem functioning

Pan

Raaijmakers

Carrión

2023

Trends in Microbiology

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Section: Association With Host Health and Diseasementioning

confidence: 99%

Importance of Bacteroidetes in host–microbe interactions and ecosystem functioning

Pan

Raaijmakers

Carrión

2023

Trends in Microbiology

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“…Hurley et al probed the chemistry of THOR and revealed a major player in genetic regulation. Koreenceine, an antibiotic produced by P. koreensis , was found to be responsible for a large portion of differentially regulated genes in the other two community members . Without the initial detection of this compound and its BGC, significant factors in community survival and interaction would be overlooked.…”

Section: Microbiome Studiesmentioning

confidence: 99%

Reducing Mass Confusion over the Microbiome

McAtamney,

Heaney,

Lizama-Chamu

et al. 2023

Anal. Chem.

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“…F. johnsoniae growth is inhibited by koreenceine, an antibiotic produced by P. koreensis , and koreenceine levels are modulated by the third THOR member, B. cereus (12). Gene expression and metabolite profiles of each member differ when they are in the community versus in pure culture (13, 14). These interactions indicate that THOR members engage in a web of biological interactions.…”

Section: Introductionmentioning

confidence: 99%

Surface colonization byFlavobacterium johnsoniaepromotes its survival in a model microbial community

Magesh,

Hurley,

Nepper

et al. 2024

Preprint

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Flavobacterium johnsoniaeis a ubiquitous soil and rhizosphere bacterium, but despite its abundance, the factors contributing to its success in communities are poorly understood. Using a model microbial community,TheHitchhikersOf the Rhizosphere (THOR), we determined the effects of colonization on fitness ofF. johnsoniaein the community. Insertion sequencing (INSeq), a massively parallel transposon mutant screen, on sterile sand identified 25 genes likely to be important for surface colonization. We constructed in-frame deletions of nine candidate genes predicted to be involved in cell membrane biogenesis, motility, signal transduction, and transport of amino acids and lipids. All mutants poorly colonized sand, glass, and polystyrene and produced less biofilm than the wild type, indicating the importance of the targeted genes in surface colonization. Eight of the nine colonization-defective mutants were also unable to form motile biofilms, or zorbs, thereby suggesting that the affected genes play a role in group movement and linking stationary and motile biofilm formation genetically. Furthermore, we showed that deletion of colonization genes inF. johnsoniaeaffected its behavior and survival in THOR on surfaces, suggesting that the same traits are required for success in a multispecies microbial community. Our results provide insight into the mechanisms of surface colonization byF. johnsoniaeand form the basis for further understanding its ecology in the rhizosphere.IMPORTANCEMicrobial communities direct key environmental processes through multispecies interactions. Understanding these interactions is vital for manipulating microbiomes to promote health in human, environmental, and agricultural systems. However, microbiome complexity can hinder our understanding of the underlying mechanisms in microbial community interactions. As a first step towards unraveling these interactions, we explored the role of surface colonization in microbial community interactions using THOR, a genetically tractable model community of three bacterial species,Flavobacterium johnsoniae,Pseudomonas koreensis,andBacillus cereus.We identifiedF. johnsoniaegenes important for surface colonization in solitary conditions and in the THOR community. Understanding the mechanisms that promote success of bacteria in microbial communities brings us closer to targeted manipulations to achieve outcomes that benefit agriculture, the environment, and human health.

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THOR’s Hammer: the Antibiotic Koreenceine Drives Gene Expression in a Model Microbial Community

Abstract: The diversity, ubiquity, and significance of microbial communities is clear. However, the predictable and reliable manipulation of microbiomes to impact human, environmental, and agricultural health remains a challenge.

Cited by 12 publications

References 69 publications

Importance of Bacteroidetes in host–microbe interactions and ecosystem functioning

Importance of Bacteroidetes in host–microbe interactions and ecosystem functioning

Reducing Mass Confusion over the Microbiome

Surface colonization byFlavobacterium johnsoniaepromotes its survival in a model microbial community

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