Thixotropic silk nanofibril-based hydrogel with extracellular matrix-like structure

Liu, Yingxin; Ling, Shengjie; Wang, Suhang; Chen, Xin; Shao, Zhengzhong

doi:10.1039/c4bm00214h

Cited by 69 publications

(72 citation statements)

References 43 publications

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“…Although a few studies have revealed thixotropic silk-based hydrogel formations, 45,46 these hydrogels were prepared in water-ethanol solvents and have not been used to release drugs for localized chemotherapy either in vitro or in vivo. Therefore, the goal of this study was to develop self-assembling silk nanofiber hydrogels with thixotropic properties in all-aqueous systems to support the injectable delivery DOX.…”

Section: Introductionmentioning

confidence: 99%

Injectable and pH-Responsive Silk Nanofiber Hydrogels for Sustained Anticancer Drug Delivery

Liu

Xiao

et al. 2016

ACS Appl. Mater. Interfaces

182

178

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Silk is useful as a drug carrier due to its biocompatibility, tunable degradation, and outstanding capacity in maintaining the function of drugs. Injectable silk hydrogels could deliver doxorubicin (DOX) for localized chemotherapy for breast cancer. To improve hydrogel properties, thixotropic silk nanofiber hydrogels in an all-aqueous solution were prepared and used to locally deliver DOX. The silk hydrogels displayed thixotropic capacity, allowing for easy injectability followed by solidification in situ. The hydrogels were loaded with DOX and released the drug over eight weeks with pH- and concentration-dependent release kinetics. In vitro and in vivo studies demonstrated that DOX-loaded silk hydrogels had good antitumor response, outperforming the equivalent dose of free DOX administered intravenously. Thixotropic silk hydrogels provide improved injectability to support sustained release, suggesting promising applications for localized chemotherapy.

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Section: Introductionmentioning

confidence: 99%

Injectable and pH-Responsive Silk Nanofiber Hydrogels for Sustained Anticancer Drug Delivery

Liu

Xiao

et al. 2016

ACS Appl. Mater. Interfaces

182

178

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“…Several recent studies dealing with ultra-low fouling surface-cell interactions focused on a specific application without aiming to generalize the observed surface-cell effects. [4,[18][19][20][21] Some complex surface-cell relationships have been recently reportedthe ultra-low fouling surface wettability (or hydrophilicity), [22,23] hydration and layer thickness, [24][25][26] zwitterionization, [27] and charge, and the presence of various functional groups [28] have been shown to influence cellular behavior. The differentiation of stem cells was investigated in dependence on surface hydrophilicity and zwitterionization of the coating.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Víšová

Smolková

Uzhytchak

et al. 2020

Macromolecular Bioscience

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Ultra‐low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra‐low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied. The state‐of‐art ultra‐low fouling surface‐grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly(N‐(2‐hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA‐co‐HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra‐low fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the surface hydration capabilities. The results demonstrate a high potential of carboxybetaine‐rich ultra‐low fouling surfaces with high hydration capabilities and minimum background signal interferences to create next‐generation bioresponsive interfaces for advanced studies of living objects.

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“…A material is said to be thixotropic when its apparent viscosity decreases with time under a constant shear rate. As for thixotropic materials, the bestknown example is the thixotropic paints and the other examples include concentrated suspensions, drilling fluids, emulsions, protein solutions, laponite, and silk nanofibril-based hydrogel [23][24][25][26] . The 3D printing head consists of a piston, a syringe, and a nozzle.…”

Section: Shear Rate In An Extrusion-based 3d Printing Processmentioning

confidence: 99%

Rheological study on 3D printability of alginate hydrogel and effect of graphene oxide

Liu

Li³

2024

IJB

197

165

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Rheological study on 3D printability of alginate hydrogel and effect of graphene oxide. © 2016 Huijun Li, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 54 RESEARCH ARTICLERheological study on 3D printability of alginate hydrogel and effect of graphene oxide Abstract: In recent years, hydrogels have been used as important biomaterials for 3D printing of three dimensional tissues or organs. The key issue for printing a successful scaffold is the selection of a material with a good printability. Rheological properties of hydrogels are believed to pay an important role in 3D printability. However the relations between rheological properties of hydrogels and 3D printability have not been extensively studied. In this study, alginate-based hydrogels were prepared as a model material for an extrusion-based printer and graphene oxide was added to modify the rheological properties and 3D printability of the hydrogels. Rheological studies were performed for the hydrogel samples with different formulas. The range of shear rates that the hydrogels suffered during the printing process was deduced. This range of shear rates helped us to select a proper shear rate to investigate the thixotropic properties of the hydrogels. Furthermore, we also defined some measureable parameters to describe and discuss the quality of 3D printing. The present study shows a new approach to analysis of 3D printability of a hydrogel and also provides some suggestion for 3D printing of 3D scaffolds.

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Thixotropic silk nanofibril-based hydrogel with extracellular matrix-like structure

Abstract: We present an injectable hydrogel based on silk fibroin (SF) nanofibrils which may offer benefits for cell encapsulation and delivery.

Cited by 69 publications

References 43 publications

Injectable and pH-Responsive Silk Nanofiber Hydrogels for Sustained Anticancer Drug Delivery

Injectable and pH-Responsive Silk Nanofiber Hydrogels for Sustained Anticancer Drug Delivery

Modulation of Living Cell Behavior with Ultra‐Low Fouling Polymer Brush Interfaces

Rheological study on 3D printability of alginate hydrogel and effect of graphene oxide

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