Thirty Years of Pear Breeding Activity at Isf Forlì, Italy

Rivalta, L.; Dradi, M.; Rosati, Carlo

doi:10.17660/actahortic.2002.596.33

Cited by 12 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a dominant dwarf mutant that affects internode length, PcDw probably is a regulator element and not a structure gene, which needs to be confirmed by other research. The fruit quality of the several selections made from 'Nain Vert' and the subsequent backcross progeny with reduced growth habit were too poor to allow the release of these genotypes, but some of them could be used as ornamental plants as a result of their compact, globe-shaped canopy (Fideghelli et al, 2003;Rivalta et al, 2002). 'Aihuali', an offspring of 'Nain Vert', is characterized by a dwarf growth habit.…”

Section: Discussionmentioning

confidence: 99%

“…However, for pear, there is a significant lack of suitable germplasm to breed dwarf scion cultivars and rootstocks. In the 1930s, a French Pyrus communis cultivar named Nain Vert, originating from a chance seedling (Fideghelli et al, 2003;Rivalta et al, 2002) and exhibiting the dwarf characteristic, was released. This cultivar forms a bush between 0.9 and 1.2 m high with very compact internode branches, and in 1991, we obtained openpollinated seeds from 'Nain Vert' from East Malling Research Station, UK.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Genetic Mapping of PcDw Determining Pear Dwarf Trait

Wang¹,

Tian²,

Buck³

et al. 2011

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

European pear (Pyrus communis) ‘Aihuali’ carrying the dwarf character originating from ‘Nain Vert’ was crossed with ‘Chili’ (Pyrus bretschneideri). A total of 352 F1 progenies was produced to investigate the inheritance of the dwarf trait, and 111 of these were used to develop molecular markers. Chi-square analysis showed that the character fitted a 1:1 ratio indicative of a single dominant gene, which we have named PcDw. Using a bulked segregant analysis approach with 500 random amplified polymorphic DNA (RAPD) and 51 simple sequence repeat (SSR) markers from pear (Pyrus pyrifolia and P. communis) and apple (Malus ×domestica), four markers were identified as cosegregating with the dwarf character. Two of these were fragments produced by the S1212 and S1172 RAPD primers, and the other two were the pear SSR markers KA14 and TsuENH022. The RAPD markers were converted into sequence-characterized amplified regions (SCARs) and designated S1212-SCAR318 and S1172-SCAR930 and, with the SSR markers KA14 and TsuENH022, were positioned 5.9, 9.5, 8.2, and 0.9 cM from the PcDw gene, respectively. Mapping of the KA14 and TsuENH022 markers enabled the location of the PcDw gene on LG 16 of the pear genetic linkage map.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Genetic Mapping of PcDw Determining Pear Dwarf Trait

Wang¹,

Tian²,

Buck³

et al. 2011

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

“…Generally, standard breeding programs still offer the possibility to release new improved pear cultivars and rootstocks but always with long production times and with the difficulty of solving specific problems of a cultivar without losing its main peculiarities. In fact, conventional breeding is difficult and demands several years because of pear, as perennial fruit species, is characterized by a high level of heterozygosity and a long juvenile period (4-6 years) (Caboni et al 1999;Rivalta et al 2002;Prohens et al 2022). Thus, New Genomic Techniques (NGTs) based on the use of different biotechnological tools offer the possibility to improve, in shorter time, the existing and well-known pear commercial rootstocks and cultivars by modifying specific traits to obtain plants with higher production capacity and fruits nutritionally improved (Sabbadini et al 2021).…”

Section: Introductionmentioning

confidence: 99%

In vitro shoot regeneration from leaves of Pyrus communis L. rootstock and cultivars

Ricci

Mezzetti

Navacchi

et al. 2023

Plant Biotechnol Rep

View full text Add to dashboard Cite

The influence of TDZ on adventitious shoot regeneration from leaves of the pear cultivars Conference and Abate Fétel, and the rootstock Farold®87 was investigated. Our main aim was to set up efficient in vitro regeneration protocols for all these pear genotypes by using expanding leaves from elongated shoot cultures as starting plant material. Our best results in terms of percentage of regeneration were achieved by using half-strength Murashige and Skoog basal medium supplemented with 1 μM NAA, combined with 13.5 μM TDZ for Conference (87.3%) and Farold®87 (53.3%), and 9 μM TDZ for Abate Fétel (68%). The impact on leaf organogenesis of the antibiotics timentin, cefotaxime, and carbenicillin, alone or in combination, usually used for the control of Agrobacterium overgrowth, and of kanamycin, commonly used for the selection of putatively transformed plants, were also evaluated to be exploited in future transformation trials. In general, the use of carbenicillin (475 mM), cefotaxime/carbenicillin (210/238 mM) and cefotaxime (630 mM) did not negatively affect the regeneration efficiency of Conference, Abate Fétel and Farold®87, respectively. The use of 4 μM kanamycin should be suitable to select transformed shoots from Abate Fétel and Farold®87 leaves, while a lower concentration or a different selection strategy should be applied for Conference. We report new regeneration and selection protocols usable for the application of new biotechnologies in the genetic improvement of pear cultivars and rootstocks.

show abstract

“…‘Le Nain Vert’, which was a mutant French cultivar of Pyrus communis , exhibited significantly dwarf characteristics 8 . In our previous study, we obtained one dwarf seedling that showed similar features to ‘Le Nain Vert’ and designated ‘Aihuali 9 ’.…”

Section: Introductionmentioning

confidence: 99%

Deletion in the Promoter of PcPIN-L Affects the Polar Auxin Transport in Dwarf Pear (Pyrus communis L.)

Zheng

Xiao

Wang

2019

Sci Rep

View full text Add to dashboard Cite

Dwarf cultivars or dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern pear production. Previously, we found that the dwarf growth habit of pear is controlled by a single dominant gene PcDw. In this study, PcPIN-L (PCP021016) was cloned from dwarf-type and standard-type pears. PcPIN-L expression was significantly lower in the dwarf-type pears than in standard-type pears, which was caused by the CT repeat deletion in the promoter of dwarf-type pears. PcPIN-L overexpression in tobacco plants enhanced the growth of the stems and the roots. Notably, the indole acetic acid (IAA) content decreased in the shoot tips and increased in the stems of transgenic lines compared with wild type, which is consistent with the greater IAA content in the shoot tips and lower IAA content in the stems of dwarf-type pears than in standard-type pears. The CT repeat deletion in the promoter that causes a decrease in promoter activity is associated with lower PcPIN-L expression in the dwarf-type pears, which might limit the polar auxin transport and in turn result in the dwarf phenotype. Taken together, the results provide a novel dwarfing molecular mechanism in perennial woody plants.

show abstract

Thirty Years of Pear Breeding Activity at Isf Forlì, Italy

Cited by 12 publications

References 0 publications

Genetic Mapping of PcDw Determining Pear Dwarf Trait

Genetic Mapping of PcDw Determining Pear Dwarf Trait

In vitro shoot regeneration from leaves of Pyrus communis L. rootstock and cultivars

Deletion in the Promoter of PcPIN-L Affects the Polar Auxin Transport in Dwarf Pear (Pyrus communis L.)

Contact Info

Product

Resources

About