Thirty years of neuroendocrinology: Technological advances pave the way for molecular discovery

Abstract

Since the 1950s, the systems level interactions between the hypothalamus, pituitary and end organs such as the adrenal, thyroid and gonads have been well known; however, it is only over the last three decades that advances in molecular biology and information technology have provided a tremendous expansion of knowledge at the molecular level. Neuroendocrinology has benefitted from developments in molecular genetics, epigenetics and epigenomics, and most recently optogenetics and pharmacogenetics. This has enabled a new understanding of gene regulation, transcription, translation and post‐translational regulation, which should help direct the development of drugs to treat neuroendocrine‐related diseases.

In situ hybridization of feline leukemia virus in a primary neural B-cell lymphoma

“…RNAscope could also be used for diagnostic purposes, in which only FFPE tissues and no clinical samples are available; however, the price of the assay may be too high for routine use, especially when compared to PCR and ELISA. RNAscope has been described for detection of RNA in various fields of research, 6,7,9,13 and our study shows its applicability in the case of an oncogenic retrovirus.…”