Thirty Years of France' Experience in the Production of Disease-Free Garlic and Shallot Mother Bulbs

Messiaen, C. M.; Lot, Hervé; Delécolle, Brigitte

doi:10.17660/actahortic.1994.358.45

Cited by 12 publications

(9 citation statements)

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“…In most countries, OYDV was the predominant virus (Van Dijk ; Messiaen et al. ; Shiboleth et al. ; Dovas and Vovlas ; Mavricˇ and Ravnikar ; Mohammad et al.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Phylogenetic Analysis of Potyviruses, Carlaviruses and Allexiviruses in Garlic in Sudan

Mohammed

Zicca²,

Manglli

et al. 2013

Journal of Phytopathology

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Allium sativum L. samples of three cultivars grown in Sudan were shown to contain several of the common garlic viruses of the genera Potyvirus, Carlavirus and Allexivirus. In particular, Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GarCLV), Garlic virus A (GVA), Garlic virus B (GVB), Garlic virus C (GVC) and Garlic virus X (GVX) were detected by reverse transcription polymerase chain reaction (RT-PCR) assay using degenerate genus-specific and virus-specific primer sets. Multiple viral infections with members of all three genera were found in all but one sample. Further, molecular and phylogenetic analysis of nucleotide and deduced amino acid sequences revealed that Sudanese isolates of LYSV, GarCLV and of Allexivirus genus were significantly divergent in partial coat protein genomic region from isolates of the same species retrieved from GenBank. This is the first report and study on viruses infecting garlic in Sudan.

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“…In most countries, OYDV was the predominant virus (Van Dijk ; Messiaen et al. ; Shiboleth et al. ; Dovas and Vovlas ; Mavricˇ and Ravnikar ; Mohammad et al.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Phylogenetic Analysis of Potyviruses, Carlaviruses and Allexiviruses in Garlic in Sudan

Mohammed

Zicca²,

Manglli

et al. 2013

Journal of Phytopathology

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“…There are limited reports of the development of symptoms indicative of viral infection, primarily chlorotic spots and yellow stripes on leaves. In spite of extensive studies in Italy (Bellardi et al, 1995), France (Messiaen, 1994), Japan (Nagakubo et al, 1994), Java (Sutarya, 1994), Australia (Sward et al, 1994) and some other Asian countries (Barg et al, 1994), no study has been conducted leading to detection and identification of garlic viruses in Bangladesh. The detection of viruses in this study will provide important information and work as a guide line for the future researches aiming at garlic improvement.…”

Section: Resultsmentioning

confidence: 99%

Detection of viruses of Bangladeshi and Japanese garlic and their elimination through root meristem culture

Haque

Hattori

2017

Progress. Agric.

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A number of viruses cause considerable yield loss and quality deterioration in garlic. Root meristems of virus infected plants are known to be free from detectable viruses. This potentiality could be exploited to obtain virus free clones at a high frequency by culturing excised root meristems in vitro. We have developed efficient methods of direct and somatic embryo derived shoot regeneration from root meristems of garlic. The objectives of this work were to detect viruses infecting Bangladeshi and Japanese garlic clones and find an easy and efficient method of eliminating the viruses for the improvement of both yield and quality of garlic. At first, we confirmed the presence of detectable viruses in three Bangladeshi and one Japanese clones. The clones were infected with four different types of viruses: Garlic viruses (GarVs), Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), and Garlic common latent virus (GCLV). To eliminate those viruses, as per our previous method, root meristems were cultured on MS medium supplemented with 1.0 µM NAA and 10.0 µM BA. Shoot primordia developed from the cultured explants within 1 month. The regenerated individual shoot buds (2-5 mm) were separated from the mother explants and transferred to growth regulators free medium. RT-PCR confirmed that the viruses present in the mother garlic plants were absent in the shoots found after two-step culture. The regenerated shoots were rooted on growth regulator free medium and transferred to pots. Results indicated that the plants remained free from LYSV. Virus elimination through root meristem culture emerged as an efficient novel technique for the eradication of multiple viruses as confirmed by RT-PCR in this study. This technique has the potential for the production and supply of virus free propagules (plants/bulblets) for the yield and quality improvement of garlic.Progressive Agriculture 28 (2): 55-63, 2017

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“…The viruses that tend to cause it severe damages are potyviruses, such as Leek Yellow Stripe Virus (LYSV), Garlic Yellow Streak Virus (GYSV) and Onion Yellow Dwarf Virus (OYDV) (Bos, 1982;Walkey, 1987). Some carlaviruses, like Common Latent Virus (GCLV) and Shallot Latent Virus (SLV) can also infect the garlic plant (Messiaen et al, 1994). One of the most widely spread diseases in garlic producing countries is white rot, caused by the fungus Sclerotium cepivorum, which provokes wilting of the plant and rotting of the bulb.…”

Section: Pests and Diseases During Garlic Cultivation And Storagementioning

confidence: 99%

Biotechnological Tools for Garlic Propagation and Improvement

Robledo-Paz¹,

Tovar-Soto²

2012

Innovations in Biotechnology

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Thirty Years of France' Experience in the Production of Disease-Free Garlic and Shallot Mother Bulbs

Cited by 12 publications

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Occurrence and Phylogenetic Analysis of Potyviruses, Carlaviruses and Allexiviruses in Garlic in Sudan

Occurrence and Phylogenetic Analysis of Potyviruses, Carlaviruses and Allexiviruses in Garlic in Sudan

Detection of viruses of Bangladeshi and Japanese garlic and their elimination through root meristem culture

Biotechnological Tools for Garlic Propagation and Improvement

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