Thirty-One Flavors of Drosophila Rab Proteins

Zhang, Jun; Schulze, Karen L.; Hiesinger, P. Robin; Suyama, Kaye; Wang, Stream; Fish, Matthew; Acar, Melih; Hoskins, Roger A.; Bellen, Hugo J.; Scott, Matthew P.

doi:10.1534/genetics.106.066761

Cited by 274 publications

(334 citation statements)

References 71 publications

(80 reference statements)

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“…We next determined if Rab11-positive endosomes are polarized. To our surprise, we found using GFP and/or YFP fusions (12,25,26) that neither Rab11 nor Rab5 are polarized, whereas Rab4 and Rab7 are partially polarized (Fig. S2).…”

Section: Rab11 Regulates the Polarization Of The Exocyst Component Sementioning

confidence: 85%

“…The generation of dominant negative rab4, rab7, and rab11 is described in SI Materials and Methods. YFP fusion to dominant negative Rab proteins, YFP and GFP fusions to wild-type Rab proteins, and dominant negative Rab protein (12,25,26) constructs were expressed in the border cell cluster using one copy of slbo-Gal4 (50) or slbo-Gal4, UAS-CD8::GFP. dsRNA constructs from the Vienna Drosophila RNAi Center (51) were expressed in flies using c306-Gal4 (52).…”

Section: Methodsmentioning

confidence: 99%

“…S1A). First, five endosomal Rab proteins (YFP::rab4 SN , YFP::rab5 SN , YFP::rab7 TN , YFP::rab11 SN , and YFP::rabX4 TN ) from the Hugo Bellen collection (12) were expressed in the border cell cluster. YFP::rab5 SN was used as a positive control, because the rab5 regulator sprint was pre-viously shown to be involved in border cell migration (10).…”

Section: Trafficking Through the Early Endosome And The Recycling Endmentioning

confidence: 99%

“…Rab5 regulates transport through the early endosome, Rab4 through the rapid recycling pathway, Rab11 through the recycling endosome, and Rab7 to late endosomes. Furthermore, another endosomal Rab protein has been recently characterized in Drosophila and was named RabX4 (12). Small GTPases can be locked into an active form or an inactive form by a single-amino-acid change.…”

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confidence: 99%

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Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration

Assaker

Ramel²,

Wculek³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

107

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Border cell migration is a stereotyped migration occurring during the development of the Drosophila egg chamber. During this process, a cluster composed of six to eight follicle cells migrates between nurse cells toward the oocyte. Receptor tyrosine kinases (RTKs) are enriched at the leading edge of the follicle cells and establish the directionality of their migration. Endocytosis has been shown to play a role in the maintenance of this polarization; however, the mechanisms involved are largely unknown. In this study, we show that border cell migration requires the function of the small GTPases Rab5 and Rab11 that regulate trafficking through the early and the recycling endosome, respectively. Expression of a dominant negative form of rab11 induces a loss of the polarization of RTK activity, which correlates with a severe migration phenotype. In addition, we demonstrate that the exocyst component Sec15 is distributed in structures that are polarized during the migration process in a Rab11-dependent manner and that the down-regulation of different subunits of the exocyst also affects migration. Together, our data demonstrate a fundamental role for a plasma membrane-endosome trafficking cycle in the maintenance of active RTK at the leading edge of border cells during their migration.

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Section: Rab11 Regulates the Polarization Of The Exocyst Component Sementioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Trafficking Through the Early Endosome And The Recycling Endmentioning

confidence: 99%

mentioning

confidence: 99%

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Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration

Assaker

Ramel²,

Wculek³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

107

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“…Rab2 and Rim both encode nervous system-specific trafficking proteins. Rab2 is a Rab GTPase that is mainly expressed in the Drosophila nervous system (Zhang et al 2007). It is required for proper vesicle transport from the endoplasmic reticulum to the Golgi.…”

mentioning

confidence: 99%

Large Neurological Component to Genetic Differences Underlying Biased Sperm Use in Drosophila

2013

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Sperm competition arises as a result of complex interactions among male and female factors. While the roles of some male factors are known, little is known of the molecules or mechanisms that underlie the female contribution to sperm competition. The genetic tools available for Drosophila allow us to identify, in an unbiased manner, candidate female genes that are critical for mediating sperm competition outcomes. We first screened for differences in female sperm storage and use patterns by characterizing the natural variation in sperm competition in a set of 39 lines from the sequenced Drosophila Genetic Reference Panel (DGRP) of wild-derived inbred lines. We found extensive female variation in sperm competition outcomes. To generate a list of candidate female genes for functional studies, we performed a genome-wide association mapping, utilizing the common single-nucleotide polymorphisms (SNPs) segregating in the DGRP lines. Surprisingly, SNPs within ion channel genes and other genes with roles in the nervous system were among the top associated SNPs. Knockdown studies of three candidate genes (para, Rab2, and Rim) in sensory neurons innervating the female reproductive tract indicate that some of these candidate female genes may affect sperm competition by modulating the neural input of these sensory neurons to the female reproductive tract. More extensive functional studies are needed to elucidate the exact role of all these candidate female genes in sperm competition. Nevertheless, the female nervous system appears to have a previously unappreciated role in sperm competition. Our results indicate that the study of female control of sperm competition should not be limited to female reproductive tract-specific genes, but should focus also on diverse biological pathways. I N many organisms, sperm competition is an important source of reproductive variation and is critical to the reproductive success of both males and females. Sperm competition occurs when a female mates with and stores sperm from multiple males. Multiple mating creates a situation in which the males' sperm "compete" for successful fertilizations. While the ultimate outcome of a sperm competition depends on a complex interaction between male and female factors, the sexes often have different interests. Males are essentially in competition with other males, and while natural selection drives males to become better competitors with each other, it may result in males damaging the female through toxicity of seminal proteins (Wigby and Chapman 2004;Mueller et al. 2007). On the other hand, females benefit from simply being able to produce the most and highest-fitness offspring, and this may entail a specific response to male-produced molecules that influence sperm competition. The sexual conflict that arises from these different evolutionary goals of males and females implies that the genetic response to selection on sperm competition may be totally distinct in males and females. It remains largely unknown what genes drive this sexual antagon...

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RNAi KNOCKDOWN OF BmRab3 LED TO LARVA AND PUPA LETHALITY IN SILKWORM Bombyx mori L.

Singh

Xin

Chen

et al. 2015

Arch Insect Biochem Physiol

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Rab3 GTPases are known to play key a role in vesicular trafficking, and express highest in brain and endocrine tissues. In mammals, Rab3 GTPases are paralogs unlike in insect. In this study, we cloned Rab3 from the silk gland tissue of silkworm Bombyx mori, and identified it as BmRab3. Our in silico analysis indicated that BmRab3 is an isoform with a theoretical isoelectric point and molecular weight of 5.52 and 24.3 kDa, respectively. Further, BmRab3 showed the C-terminal hypervariability for GGT2 site but having two other putative guanine nucleotide exchange factor/GDP dissociation inhibitor interaction sites. Multiple alignment sequence indicated high similarities of BmRab3 with Rab3 isoforms of other species. The phylogeny tree showed BmRab3 clustered between the species of Tribolium castaneum and Aedes aegypti. Meanwhile, the expression analysis of BmRab3 showed the highest expression in middle silk glands (MSGs) than all other tissues in the third day of fifth-instar larva. Simultaneously, we showed the differential expression of BmRab3 in the early instar larva development, followed by higher expression in male than female pupae. In vivo dsRNA interference of BmRab3 reduced the expression of BmRab3 by 75% compared to the control in the MSGs in the first day. But as the worm grew to the third day, the difference of BmRab3 between knockdown and control was only about 10%. The knockdown later witnessed underdevelopment of the larvae and pharate pupae lethality in the overall development of silkworm B. mori L.

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Thirty-One Flavors of Drosophila Rab Proteins

Cited by 274 publications

References 71 publications

Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration

Spatial restriction of receptor tyrosine kinase activity through a polarized endocytic cycle controls border cell migration

Large Neurological Component to Genetic Differences Underlying Biased Sperm Use in Drosophila

RNAi KNOCKDOWN OF BmRab3 LED TO LARVA AND PUPA LETHALITY IN SILKWORM Bombyx mori L.

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