Thirty days after anterior cruciate ligament transection is sufficient to induce signs of knee osteoarthritis in rats: pain, functional impairment, and synovial inflammation

Barbosa, Germanna Medeiros; Cunha, Jonathan Emanuel; Russo, Thiago Luiz; Cunha, Thiago M.; Castro, Paula Aiello Tomé de Souza; Oliveira, Francisco Fábio Bezerra de; Cunha, Fernando Q.; Ramalho, Fernando Silva; Salvini, Tania F.

doi:10.1007/s00011-020-01317-1

Cited by 9 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a biphasic response was observed in a rat ACLT + MMx model with increased pain observed in the initial 5 weeks and around 10 weeks postsurgery 26 . Although synovitis and decreased functional performance were observed in another surgical rat ACLT model as early as 30 days postoperatively, the paw print area and PWT differences between operated and control animals started to converge at 60 days post‐ACLT 40 . Thus, a more severe model, such as ACLT + MMx, might be needed to observe late‐phase functional differences arising from PTOA.…”

Section: Discussionmentioning

confidence: 97%

“…Another limitation of this study is that the parasagittal sectioning plane used for histopathological analysis prohibited assessment of osteophyte formation, which is commonly associated with OA development in rats. 41 With respect to the intra-articular injection volume study, we cannot rule out of the fact that cadaver tissues may be more friable than tissues in live rats, with an increased likelihood of leakage due to tearing of the joint capsule.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of osteoarthritis functional outcomes and intra‐articular injection volume in the rat anterior cruciate ligament transection model

Wang

Wagner

et al. 2022

Journal Orthopaedic Research

View full text Add to dashboard Cite

The rat surgical anterior cruciate ligament transection (ACLT) model is commonly used to investigate intra‐articular osteoarthritis (OA) therapies, and histological assessment is often the primary outcome measure. However, histological changes do not always correlate well with clinical outcomes. Therefore, this study evaluated functional outcomes in the rat surgical ACLT model and compared intra‐articular injection volumes ranging from 20 to 50 μl. Unilateral ACLT was surgically induced and static weight‐bearing, mechanical allodynia, motor function, and gait were assessed in four groups of male, Sprague‐Dawley rats (n = 6 per group). Intra‐articular injections of 20 µl Dulbecco's phosphate‐buffered saline (DPBS), 50 µl DPBS, or 50 µl of synthetic biomimetic boundary lubricant were administered once weekly for 3 weeks postoperatively. Structural changes were evaluated histologically at 20 weeks. Rat cadaver knees were injected with 20, 30, 40, or 50 µl of gadolinium solutions and were imaged using magnetic resonance imaging (MRI). Static weight‐bearing, mechanical allodynia, and gait parameters in ACLT groups revealed differences from baseline and naïve controls for 4 weeks post‐ACLT; however, these differences did not persist beyond 6 weeks. Different intra‐articular DPBS injection volumes did not result in functional or histological changes; however, peri‐articular leakage was documented via MRI following 50, 40, and 30 µl but not 20 µl gadolinium injections. Statement of clinical significance: Differences in functional parameters were predominantly restricted to early, postoperative changes in the rat surgical ACLT model despite evidence of moderate histologic OA at 20 weeks. Injection volumes of 20–30 µl are more appropriate for investigating intra‐articular therapies in the rat knee.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Assessment of osteoarthritis functional outcomes and intra‐articular injection volume in the rat anterior cruciate ligament transection model

Wang

Wagner

et al. 2022

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Factors such as cytokine production, reactive oxygen species, and biological cell infiltration are also likely to influence graft integrity in combination with the possibility of subclinical infection. 2,3,26 More work is needed to determine if these results are applicable to other ACL graft options and the in vivo joint environment.…”

Section: Discussionmentioning

confidence: 99%

Influence of Staphylococcus epidermidis on Collagen Crimp Patterns of Soft Tissue Allograft

Blunt,

Bentkowski,

Milliron

et al. 2023

Am J Sports Med

View full text Add to dashboard Cite

Background: Postoperative infections, commonly from Staphylococcus epidermidis, may result in anterior cruciate ligament graft failure and necessitate revision surgery. In biomechanical studies, S. epidermidis has been shown to establish biofilms on tendons and reduce graft strength. Purpose/Hypothesis: The goal of this study was to determine the effect of bacterial bioburden on the collagen structure of tendon. It was hypothesized that an increase in S. epidermidis biofilm would compromise tendon crimp, a pattern necessary for mechanical integrity, of soft tissue allografts. Study design: Controlled laboratory study. Methods: Cultures of S. epidermidis were used to inoculate tibialis anterior cadaveric tendons. Conditions assessed included 5 × 105 colony-forming units or concentrated spent media from culture (no living bacteria). Incubation times of 30 minutes, 3 hours, 6 hours, and 24 hours were utilized. Second-harmonic generation imaging allowed for visualization of collagen autofluorescence. Crimp lengths were determined using ImageJ and compared based on incubation time. Results: Incubation time positively correlated with increasing S. epidermidis bioburden. Both fine and coarse crimp patterns lengthened with increasing incubation time. Significant coarse crimp changes were observed after only 30-minute incubations ( P < .029), whereas significant fine crimp lengthening occurred after 6 hours ( P < .0001). No changes in crimp length were identified after incubation in media lacking living bacteria. Conclusion: The results of this study demonstrate that exposure to S. epidermidis negatively affects collagen crimp structure. Structural alterations at the collagen fiber level occur within 30 minutes of exposure to media containing S. epidermidis. Clinical Relevance: Our study highlights the need for antimicrobial precautions to prevent graft colonization and maximize graft mechanical strength.

show abstract

“…Joint thickness of the knee was analyzed under anesthesia (O 2 2.0 L/m, 2% isoflurane) using an electronic digital caliper (Mitutoyo Absolute Digimatic 150 mm, Japan) and measured in the initial and final periods of experimental analysis in the same conditions. Three measurements were performed in the same area for each joint, and results were expressed in mm [12,21,22].…”

Section: Joint Swelling (Edema)mentioning

confidence: 99%

“…Knee skin temperature was analyzed under anesthesia (O 2 2.0 L/m, 2% isoflurane) using an infrared digital thermometer (-18 to 260˚C; Wurth Temp, Cotia, Brazil) in the initial and final periods of experimental analysis. Three measurements were performed in the same area for each joint, and results were expressed in˚C [15,21].…”

Section: Knee Skin Temperaturementioning

confidence: 99%

Clinical-like cryotherapy in acute knee arthritis of the knee improves inflammation signs, pain, joint swelling, and motor performance in mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

To assess the effects of clinical-like cryotherapy on inflammatory signs (in vivo neutrophil migration, cytokines, and joint inflammation), pain, joint swelling, balance, and motor coordination in mice with knee arthritis. Young C57BL/6 mice were randomly divided into three groups (8 to 10 mice per group): Control group: mice with no intervention; antigen-induced arthritis (AIA) group: mice sensitized and immunized with intra-articular (i.a.) injection of methylated bovine serum albumin (mBSA); and AIA + cryotherapy group: mice sensitized, immunized with i.a. injection of mBSA, and submitted to a clinical-like cryotherapy protocol. After 21 days of sensitization, AIA and AIA + cryotherapy groups received i.a. injection of mBSA (100 μg/joint) to induce joint inflammation, and a clinical-like cryotherapy protocol was applied to AIA + cryotherapy group (crushed ice bag, two cryotherapy sessions of 20 min every two hours). Experimental analysis was conducted in the initial (immediately after i.a. injection of mBSA) and final periods (two hours after the second cryotherapy session). The number of synovial fluid neutrophils, cytokine levels, joint histology, pain, joint swelling, and motor performance were also analyzed. Our results showed that clinical-like cryotherapy in mice with acute knee arthritis reduced inflammatory signs, pain, and joint swelling, and improved balance and motor coordination.

show abstract

Thirty days after anterior cruciate ligament transection is sufficient to induce signs of knee osteoarthritis in rats: pain, functional impairment, and synovial inflammation

Cited by 9 publications

References 45 publications

Assessment of osteoarthritis functional outcomes and intra‐articular injection volume in the rat anterior cruciate ligament transection model

Assessment of osteoarthritis functional outcomes and intra‐articular injection volume in the rat anterior cruciate ligament transection model

Influence of Staphylococcus epidermidis on Collagen Crimp Patterns of Soft Tissue Allograft

Clinical-like cryotherapy in acute knee arthritis of the knee improves inflammation signs, pain, joint swelling, and motor performance in mice

Contact Info

Product

Resources

About