Third order derivative spectrophotometric determination of ascorbic acid in fruits and vegetables

Özgür, Mahmure Üstün; Sungur, Sıdıka

doi:10.1016/0039-9140(95)01622-8

Cited by 19 publications

(6 citation statements)

References 15 publications

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“…Ascorbic acid content was determined by a spectrophotometrical method (Özgür & Sungur, 1995). A 50‐g sample was stirred with 100 mL of 3% (w/v) metaphosphoric acid and 8% (v/v) acetic acid solution for 45 min.…”

Section: Methodsmentioning

confidence: 99%

Influence of processing and storage on the antioxidant activity of apple derivatives

Sacchetti

Cocci

Pinnavaia

et al. 2008

Int J of Food Sci Tech

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The antioxidant activity of seven apple varieties was determined on fresh fruits and processed products (driedfruits and purees) and during storage. The antioxidant activity of apples correlated with the total phenolconcentration but not with ascorbic acid concentration. Apple derivatives showed a lower antioxidantactivity than the counterparts of the fresh fruits and the occurrence of browning phenomena duringprocessing resulted in lower antioxidant activity. Dried fruits showed higher antioxidant activity per gram ofdried matter than fruit purees probably because of the lower total thermal effect of processing. Theantioxidant activity of all products increased within the first month of storage, and then declined. The extentof early increase was related to the polyphenol content of the fruit. Packaging conditions further influencedthe antioxidant activity increase during storage possibly by affecting the oxygen availability.[...

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Section: Methodsmentioning

confidence: 99%

Influence of processing and storage on the antioxidant activity of apple derivatives

Sacchetti

Cocci

Pinnavaia

et al. 2008

Int J of Food Sci Tech

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“…Ascorbic acid (Vitamin C) Ascorbic acid extraction was done according to Özgür and Sungur (1995) with the following modifications; 100 mg of freeze dried sample were mixed with 5 mL of extraction agitated for 30 min, and centrifuged at 3000 g for 10 min, and the supernatant was collected. Solution absorbance was measured immediately at 535 and 700 nm.…”

Section: Mineral Contentmentioning

confidence: 99%

Nutritional and Nutraceutical Components of Commercial Eggplant Types Grown in Sinaloa, Mexico

Niño-Medina

Muy-Rangel

Gardea

et al. 2014

Not Bot Horti Agrobo

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The nutrimental composition and main nutraceutical components were determined in Chinese, Philippine, American, Hindu and Thai eggplant (Solanum melongena L.) types grown in Sinaloa, Mexico. Thai type showed the highest amount of protein (0.90%), crude (1.54%) and dietary (3.93%) fibre and the highest concentrations of total soluble phenolics (2049 mgCAE 100g-1) and chlorogenic acid (1700 mg 100g-1). Hindu type obtained the highest content of the minerals potassium (191.19 mg 100g-1), calcium (59.63 mg 100g-1), phosphorus (33.52 mg 100g-1), magnesium (28.96 mg 100g-1), manganese (0.44 mg 100g-1), zinc (0.78 mg 100g-1), in addition this eggplant type showed the highest levels of ascorbic acid (22 mg 100g-1). Philippine type showed the highest concentration in anthocyanins (161.10 mgC3GE 100g-1) and the highest levels of radical scavenging activity in DPPH (92.50% of inhibition) and ORAC (538.90 μmolTE g-1). Nutrient components with functional properties like biologically essential minerals, dietary fibre, ascorbic acid (vitamin C) and soluble phenols were found in higher amounts in the five eggplant types analyzed, as compared with reports from other parts in the world available in the literature.

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“…Spectrophotometry ( − ), differential pulse polarography (), and adsorption potentiometry () have been used to determine both AA and DHA (upon reduction to AA) in fruit juices and other industrial products. However, these methods lack the adequate selectivity to analyze vascular and nonvascular plant extracts ( , ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Herrero‐Martínez¹,

Simó‐Alfonso²,

Ramis‐Ramos³

et al. 2000

Environ. Sci. Technol.

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Simultaneous determination of ascorbic acid (AA), glutathione (GSH), and oxidized glutathione (GSSG) in vascular plants has been achieved by capillary zone electrophoresis. A background electrolyte containing a phosphate buffer of pH 7 and hexadimethrin bromide for dynamic control of electroosmotic flow was used. Separation was performed in less than 5 min. The signal was simultaneously monitored at 265 and 200 nm. Dehydroascorbic acid (DHA) was determined in another aliquot of the same plant extract upon reduction with dl-homocysteine. Limits of detection of AA, GSH, and GSSG ranged from 0.2 to 0.8 μg mL-1, which corresponded from 0.04 to 0.16 mg per 100 g fresh weight of tomato, watermelon, and potato leaves. Plants exposed to a high ozone concentration (1600 μg m-3) in a laboratory fumigation system during 1, 2, 3, and 5 h showed progressive ozone-induced oxidative stress. This could be seen as a simultaneous decrease of the AA/(AA+DHA) and GSH/(GSH+GSSG) ratios; however, the decline rate was higher for AA/(AA+DHA) than for GSH/(GSH+GSSG) in plants cultivated in the field, in open-top chambers, during ca. 50 days with much lower ozone concentrations (mean concentrations, 10 and 80 μg m-3).

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Third order derivative spectrophotometric determination of ascorbic acid in fruits and vegetables

Cited by 19 publications

References 15 publications

Influence of processing and storage on the antioxidant activity of apple derivatives

Influence of processing and storage on the antioxidant activity of apple derivatives

Nutritional and Nutraceutical Components of Commercial Eggplant Types Grown in Sinaloa, Mexico

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

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