Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis

Aviles-Espinosa, Rodrigo; Santos, Susana I. C. O.; Brodschelm, A.; Kaenders, Wilhelm; Alonso-Ortega, Cesar; Artigas, David; Loza-Álvarez, Pablo

doi:10.1117/1.3477535

Cited by 28 publications

(26 citation statements)

References 35 publications

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“…These wavelengths could be challenging or impossible to reach with Ti:Sapphire based laser systems. In addition, the excitation wavelength above 1 micron, compared to 800 nm excitation wavelength, facilitates the detection due to higher camera sensitivities for the SHG and THG imaging modalities [16].…”

Section: Introductionmentioning

confidence: 99%

Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron

Resan¹,

Aviles-Espinosa²,

Kurmulis³

et al. 2014

Opt. Express

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Abstract:We developed a low-cost, low-noise, tunable, high-peak-power, ultrafast laser system based on a SESAM-modelocked, solid-state Yb tungstate laser plus spectral broadening via a microstructured fiber followed by pulse compression. The spectral selection, tuning, and pulse compression are performed with a simple prism compressor. The output pulses are tunable from 800 to 1250 nm, with the pulse duration down to 25 fs, and average output power up to 150 mW, at 80 MHz pulse repetition rate. We introduce the figure of merit (FOM) for the two-photon and multiphoton imaging (or other nonlinear processes), which is a useful guideline in discussions and for designing the lasers for an improved microscopy signal. Using a 40 MHz pulse repetition rate laser system, with twice lower FOM, we obtained high signal-to-noise ratio two-photon fluorescence images with or without averaging, of mouse intestine section and zebra fish embryo. The obtained images demonstrate that the developed system is capable of nonlinear (TPE, SHG) imaging in a multimodal operation. The system could be potentially used in a variety of other techniques including, THG, CARS and applications such as nanosurgery. 1119-1121 (2000). 9. F. Druon, F. Balembois, and P. Georges, "New materials for short-pulse amplifiers," IEEE Photon. J. 3(2), 268-273 (2011). 10. S. Ricaud, A. Jaffres, K. Wentsch, A. Suganuma, B. Viana, P. Loiseau, B. Weichelt, M. Abdou-Ahmed, A. Voss, T. Graf, D. Rytz, C. Hönninger, E. Mottay, P. Georges, and F. Druon, "Femtosecond Yb:CaGdAlO4 thin-disk oscillator," Opt. Lett. 37(19), 3984-3986 (2012 Matuschek, and J. Aus der Au, "Semiconductor saturable absorber mirrors (SESAMs) for femtosecond to nanosecond pulse generation in solid-state lasers," IEEE ©2014 Optical Society of America

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Section: Introductionmentioning

confidence: 99%

Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron

Resan¹,

Aviles-Espinosa²,

Kurmulis³

et al. 2014

Opt. Express

Self Cite

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“…However, a faster and less invasive scheme is desired to make the process more efficient. Multi-photon microscopy (MPM) has been recognized as a promising technique for label-free imaging of biological samples [1,2]. Various nonlinear imaging modalities such as third harmonic generation (THG), second harmonic generation (SHG), two and three photon excited auto-fluorescence (2PEF/3PEF) can provide valuable information about intrinsic properties of the biological samples which can be employed in diagnosis applications [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Compact fiber-based multi-photon endoscope working at 1700 nm

Akhoundi

Qin

Peyghambarian

et al. 2018

Biomed. Opt. Express

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Abstract:We present the design, implementation and performance analysis of a compact multi-photon endoscope based on a piezo electric scanning tube. A miniature objective lens with a long working distance and a high numerical aperture (≈ 0.5) is designed to provide a diffraction limited spot size. Furthermore, a 1700 nm wavelength femtosecond fiber laser is used as an excitation source to overcome the scattering of biological tissues and reduce water absorption. Therefore, the novel optical system along with the unique wavelength allows us to increase the imaging depth. We demonstrate that the endoscope is capable of performing third and second harmonic generation (THG/SHG) and three-photon excitation fluorescence (3PEF) imaging over a large field of view (> 400 µm) with high lateral resolution (2.2 µm). The compact and lightweight probe design makes it suitable for minimally-invasive in-vivo imaging as a potential alternative to surgical biopsies.

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“…SHG can be observed in well-defined and highly arranged non-centrosymmetric structures such as collagen, muscle, and microtubules [12] whereas THG is observed when the focused ultra-short beam finds an interface. THG imaging has been successfully used to image live processes from various model organisms such as Caenorhabditis elegans (C. elegans), Drosophila melanogaster, and Danio rerio containing different structures such as lipid droplets, cell boundaries, and tissues [13][14][15][16][17][18][19][20].…”

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confidence: 99%

“…Therefore, other sources have also been tested. These include optical parametric oscillators (OPOs) [7,16] fiber lasers [8,9,14,[21][22][23], Cr:forsterite lasers [8,24], ytterbium laser oscillators [13,18], and semiconductor laser diodes (LDs) [25][26][27][28][29][30][31][32]. In these systems, the interplay in pulse duration, repetition rate, and average and peak power have been taken into account to achieve an efficient NL signal suitable for NL imaging.…”

mentioning

confidence: 99%