Thiouridine residues in tRNAs are responsible for a synergistic effect of UVA and UVB light in photoinactivation of <i>Escherichia coli</i>

Probst-Rüd, Sandra; McNeill, Kristopher; Ackermann, Martin

doi:10.1111/1462-2920.13319

Cited by 36 publications

(20 citation statements)

References 37 publications

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“…1 (b)). Sunlight mediated inactivation of non-antibiotic resistant E. coli was also reported in previous studies and was mainly attributed to the DNA damage initiated by UVA and UVB portions of sunlight ( Kadir and Nelson, 2014 ;Probst-Rüd et al, 2017 ).…”

Section: Photo-inactivation Of Ar E Colisupporting

confidence: 71%

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

Zhang

Liu

et al. 2020

Water Research

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The transmission of antibiotic resistance in surface water has attracted much attention due to its increasing threat to human health. The role of sunlight irradiation and the effect of dissolved organic matter (DOM) on the transmission of antibiotic resistance are still unclear. In this study, photo-inactivation of antibiotic resistant bacteria (ARB) was investigated using antibiotic resistant E. coli (AR E. coli ) that contained the tetracycline resistance gene (Tc-ARG) as a representative. The results showed that AR E. coli underwent significant photo-inactivation due to the membrane damage induced by direct irradiation and by the generated reactive oxygen species. Simulated sunlight irradiation specifically suppressed the expression of tetracycline resistance, which is attributed to the destruction of tetracycline-specific efflux pump. Tetracycline inhibited the photo-inactivation of AR E. coli due to its selective pressure on tetracycline resistant E. coli and competitive light absorption effect. Suwannee River fulvic acid (SRFA), a representative DOM, promoted the inactivation of AR E. coli and further inhibited the expression of tetracycline resistance gene due to the generation of its excited triplet state, singlet oxygen, and hydroxyl radical. The extracellular Tc-ARG also underwent fast photodegradation under light irradiation and in the presence of SRFA, which leads to the decrease of its transformation efficiency. This study provided insight into the sunlight-induced inactivation of ARB, which is of significance for understanding the transmission of tetracycline resistance in surface water.

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Section: Photo-inactivation Of Ar E Colisupporting

confidence: 71%

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

Zhang

Liu

et al. 2020

Water Research

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“…Previous studies report that UV-B radiation is approximately 100–1000 times more lethal for microorganisms than UV-A [27]. It is well known that the UV-B germicidal effect is mainly based on the direct effect on DNA stability as it overlaps with the DNA absorption region.…”

Section: Discussionmentioning

confidence: 99%

Microbiological Evaluation of 5 L- and 20 L-Transparent Polypropylene Buckets for Solar Water Disinfection (SODIS)

et al. 2019

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Background: Solar water disinfection (SODIS) is an appropriate technology for household treatment of drinking water in low-to-middle-income communities, as it is effective, low cost and easy to use. Nevertheless, uptake is low due partially to the burden of using small volume polyethylene terephthalate bottles (1.5–2 L). A major challenge is to develop a low-cost transparent container for disinfecting larger volumes of water. (2) Methods: This study examines the capability of transparent polypropylene (PP) buckets of 5 L- and 20 L- volume as SODIS containers using three waterborne pathogen indicators: Escherichia coli, MS2-phage and Cryptosporidium parvum. (3) Results: Similar inactivation kinetics were observed under natural sunlight for the inactivation of all three organisms in well water using 5 L- and 20 L-buckets compared to 1.5 L-polyethylene-terephthalate (PET) bottles. The PP materials were exposed to natural and accelerated solar ageing (ISO-16474). UV transmission of the 20 L-buckets remained stable and with physical integrity even after the longest ageing periods (9 months or 900 h of natural or artificial solar UV exposure, respectively). The 5 L-buckets were physically degraded and lost significant UV-transmission, due to the thinner wall compared to the 20 L-bucket. (4) Conclusion: This work demonstrates that the 20 L SODIS bucket technology produces excellent bacterial, viral and protozoan inactivation and is obtained using a simple transparent polypropylene bucket fabricated locally at very low cost ($2.90 USD per unit). The increased bucket volume of 20 L allows for a ten-fold increase in treatment batch volume and can thus more easily provide for the drinking water requirements of most households. The use of buckets in households across low to middle income countries is an already accepted practice.

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“…UVA is known to be less effective at killing bacteria than UVB or UVC. Probst-Rüd et al (2017) found that UVA radiation mainly induced sublethal effects, such as oxidative stress, protein damage, growth delay and energy metabolism reduction, which could damage cells rather than kill them. In this case, damaged cells can recover and functionalize normally under appropriate conditions, which should be avoided in experiments and applications.…”

Section: Factors Influencing Pdimentioning

confidence: 99%

The application of photodynamic inactivation to microorganisms in food

et al. 2021

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Thiouridine residues in tRNAs are responsible for a synergistic effect of UVA and UVB light in photoinactivation of Escherichia coli

Cited by 36 publications

References 37 publications

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

Microbiological Evaluation of 5 L- and 20 L-Transparent Polypropylene Buckets for Solar Water Disinfection (SODIS)

The application of photodynamic inactivation to microorganisms in food

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