Thiosulfate and tetrathionate degradation as well as biofilm generation by Thiobacillus intermedius and Thiobacillus versutus studied by microcalorimetry, HPLC, and ion-pair chromatography

Wentzien, Susanne; Sand, W.; Albertsen, Angela; Steudel, Ralf

doi:10.1007/s002030050030

Cited by 8 publications

(11 citation statements)

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“…There is evidence that thiosulfate instead of sulfur is the primary product of microbial pyrite leaching under acidic conditions (48). This finding suggests the existence of an altered sulfur chemistry based on sulfane monosulfonic acids (55,60). Thiosulfate undergoes a sequence of chemical reactions which finally may give rise to the easily detectable and slowly biodegradable sulfur.…”

Section: Discussionmentioning

confidence: 98%

Microbial diversity in uranium mine waste heaps

Schippers¹,

Hallmann²,

Wentzien³

et al. 1995

Appl Environ Microbiol

141

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Two different uranium mine waste heaps near Ronneburg, Thuringia, Germany, which contain the remains of the activity of the former uranium-mining Soviet-East German company Wismut AG, were analyzed for the occurrence of lithotrophic and chemoorganotropic leach bacteria. A total of 162 ore samples were taken up to a depth of 5 m. Cell counts of ferrous iron-, sulfur-, sulfur compound-, ammonia-, and nitrite-oxidizing bacteria were determined quantitatively by the most-probable-number technique. Sulfate-, nitrate-, ferric iron-, and manganese-reducing bacteria were also detected. In addition, the metabolic activity of sulfur-and ironoxidizing bacteria was measured by microcalorimetry. Generally, all microorganisms mentioned above were detectable in the heaps. Aerobic and anaerobic microorganisms thrived up to a depth of 1.5 to 2 m. Up to 99% of Thiobacillus ferrooxidans cells, the dominant leaching bacteria, occurred to this depth. Their numbers correlated with the microbial activity measurements. Samples below 1.5 to 2 m exhibited reduced oxygen concentrations and reduced cell counts for all microorganisms.

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Section: Discussionmentioning

confidence: 98%

Microbial diversity in uranium mine waste heaps

Schippers¹,

Hallmann²,

Wentzien³

et al. 1995

Appl Environ Microbiol

141

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show abstract

“…In the ensuing reaction, tetrathionate decomposes (in the presence of pyrite) to elemental sulfur, sulfate, and traces of tri-and pentathionate. The occurrence of these compounds is the result of a reaction mechanism involving disulfane-monosulfonic acid (30,35; for a review, see reference 20). Tetrathionate is hydrolyzed to highly reactive disulfane-monosulfonic acid and sulfate (equation 6).…”

Section: Discussionmentioning

confidence: 99%

“…According to this account, thiosulfate and iron(II) ions are formed as the first intermediates. As has been shown, thiosulfate undergoes a series of reactions in acidic solutions starting from disulfane-monosulfonic acid and giving rise to polythionates, elemental sulfur, and sulfite (30,35).…”

mentioning

confidence: 99%

Sulfur chemistry in bacterial leaching of pyrite

Schippers¹,

Jozsa²,

Sand³

1996

Appl Environ Microbiol

323

114

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In the case of pyrite bioleaching by Leptospirillum ferrooxidans, an organism without sulfur-oxidizing capacity, besides the production of tetra-and pentathionate, a considerable accumulation of elemental sulfur occurred. A similar result was obtained for chemical oxidation assays with acidic, sterile iron(III) ioncontaining solutions. In the case of Thiobacillus ferrooxidans, only slight amounts of elemental sulfur were detectable because of the organism's capacity to oxidize sulfur compounds. In the course of oxidative, chemical pyrite degradation under alkaline conditions, the accumulation of tetrathionate, trithionate, and thiosulfate occurred. The data indicate that thiosulfate, trithionate, tetrathionate, and disulfane-monosulfonic acid are key intermediate sulfur compounds in oxidative pyrite degradation. A novel (cyclic) leaching mechanism is proposed which basically is indirect.

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“…The unstable sulfane‐monosulfonate was proposed as an intermediate in chain elongation leading to the production of other polythionates (tri‐, penta‐ and hexathionate). HS 2 SO 3 – is further dissociated to S 2 SO 3 2– and both forms chemically react with tetrathionate, leading to the formation of thiosulfate and pentathionate [24]. Meulenberg et al .…”

mentioning

confidence: 99%

Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus

Bugaytsova

Lindström

2003

European Journal of Biochemistry

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The moderately thermophilic bacterium Acidithiobacillus caldus is found in bacterial populations in many bioleaching operations throughout the world. This bacterium oxidizes elemental sulfur and other reduced inorganic sulfur compounds as the sole source of energy. The purpose of this study was to purify and characterize the tetrathionate hydrolase of A. caldus. The enzyme was purified 16.7-fold by one step chromatography using a SP Sepharose column. The purified enzyme resolved into a single band in 10% polyacrylamide gel, both under denaturing and native conditions. Its homogeneity was confirmed by N-terminal amino acid sequencing. Tetrathionate hydrolase was shown to be a homodimer with a molecular mass of 103 kDa (composed from two 52 kDa monomers). The purified enzyme had optimum activity at pH 3.0 and 40°C and an isoelectric point of 9.8. The periplasmic localization of the enzyme was determined by differential fractionation of A. caldus cells. Detected products of the tetrathionate hydrolase reaction were thiosulfate and pentathionate as confirmed by RP-HPLC analysis. The activity of the purified enzyme was drastically enhanced by divalent metal ions.

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Thiosulfate and tetrathionate degradation as well as biofilm generation by Thiobacillus intermedius and Thiobacillus versutus studied by microcalorimetry, HPLC, and ion-pair chromatography

Cited by 8 publications

References 0 publications

Microbial diversity in uranium mine waste heaps

Microbial diversity in uranium mine waste heaps

Sulfur chemistry in bacterial leaching of pyrite

Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus

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