Thioredoxins regulate the metabolic fluxes throughout the tricarboxylic acid cycle and associated pathways in a light-independent manner

Porto, Nicole P.; Bret, Raissa Souza Caminha; Souza, Paulo V L; Cândido‐Sobrinho, Silvio A.; Medeiros, David B.; Fernie, Alisdair R.; Daloso, Danilo de Menezes

doi:10.1016/j.plaphy.2022.10.022

Cited by 5 publications

(1 citation statement)

References 97 publications

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“…Additionally, the compounds that were correlated with these genes in the six pathways, namely, succinate, methylmalonate, and 3-hydroxypropionate, were considerably enriched, with succinate accounting for the highest proportion. Thioredoxin h2 and the mitochondrial thioredoxin system regulate the metabolic fluxes throughout the tricarboxylic acid cycle and associated pathways (glycolysis, gluconeogenesis and the synthesis of glutamine) [91]. The F-box targets citrate synthase 2 for proteasomal degradation, thereby suppressing the glyoxylate cycle, an anabolic pathway that replenishes the TCA cycle with succinate for the activation of gluconeogenesis [92].…”

Section: Model-based Prediction Of Transcripts and Metabolites Relate...mentioning

confidence: 99%

Comparative Physiological, Transcriptomic, and Metabolomic Analyses of Acacia mangium Provide New Insights into Its Molecular Mechanism of Self-Incompatibility

Zhang,

Huang,

Zeng

2023

Forests

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Acacia mangium is well known as a valuable commercial tree species in the Acacia genus. A. mangium was recently found to be self-incompatible (SI), but its SI mechanism is not clear, which has hindered the progress of genetic improvement of A. mangium with strong resistance. To confirm the SI type of A. mangium, pollen germination was observed via fluorescence microscopy at 0 h, 3 h, 6 h, 9 h, 12 h, and 24 h after self-pollination. We found over ninety percent of the pollen grains produced no pollen tube growth on the stigma/style. To further explore the SI molecular mechanism of A. mangium, tests of the transcriptome and metabolome were carried out after self-pollination. Observations of pollen germination after self-pollination using fluorescence microscopy suggested that the SI type of A. mangium is gametophytic self-incompatibility (GSI). A combined transcriptomic and metabolomic analysis showed that DEGs (differentially expressed genes) related to SI (6 S-glycoproteins, 93 F-box proteins, 69 26S proteasomes, 38 calcium-dependent protein kinases/calmodulin and 41 thioredoxin genes) were significantly enriched in six KEGG (sulfur metabolism, tyrosine metabolism, phenylalanine metabolism, butanoate metabolism, and valine, leucine, and isoleucine degradation). Further analysis of these six pathways revealed the enrichment of SI-related DEGs corresponding to succinate, methylmalonate, and 3-hydroxypropane. These three metabolites were significantly downregulated. The analysis of transcripts and metabolites suggested that transcripts of SI-related gene families (thioredoxin and F-box protein) were significantly upregulated under the regulation of transcription factors (TFs) after self-pollination, leading to a decrease in metabolites (such as succinate, methylmalonate, and 3-hydroxypropionate). We also further speculated that TFs (MYB, HB-HD-ZIP, AP2/ERF-ERF, and bZIP) and gene families (thioredoxin and F-box protein) were important factors related to the SI of A. mangium.

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Section: Model-based Prediction Of Transcripts and Metabolites Relate...mentioning

confidence: 99%

Comparative Physiological, Transcriptomic, and Metabolomic Analyses of Acacia mangium Provide New Insights into Its Molecular Mechanism of Self-Incompatibility

Zhang,

Huang,

Zeng

2023

Forests

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Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco

Nie,

Qiao,

et al. 2024

Plant Cell Tiss Organ Cult

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U-13C-glucose incorporation into source leaves of Brassica napus highlights light-dependent regulations of metabolic fluxes within central carbon metabolism

Dellero,

Berardocco,

Bouchereau

2024

Journal of Plant Physiology

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Thioredoxins regulate the metabolic fluxes throughout the tricarboxylic acid cycle and associated pathways in a light-independent manner

Cited by 5 publications

References 97 publications

Comparative Physiological, Transcriptomic, and Metabolomic Analyses of Acacia mangium Provide New Insights into Its Molecular Mechanism of Self-Incompatibility

Comparative Physiological, Transcriptomic, and Metabolomic Analyses of Acacia mangium Provide New Insights into Its Molecular Mechanism of Self-Incompatibility

Tomato SlNTRB improved excess nitrate stress tolerance by alleviating the oxidative damage in transgenic tobacco

U-13C-glucose incorporation into source leaves of Brassica napus highlights light-dependent regulations of metabolic fluxes within central carbon metabolism

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