Thiopseudomonas denitrificans gen. nov., sp. nov., isolated from anaerobic activated sludge

Tan, Wenfa; Zhao, Jiuran; Chen, Chuan; Yang, Yuan; Gao, Lingfang; Wang, Hongfei; Cheng, Juan; Li, Wenjun; Wang, Aijie

doi:10.1099/ijs.0.064634-0

Cited by 50 publications

(20 citation statements)

References 22 publications

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“…It had the ability to hydrolyze lipid and denitrify the nitrate nitrogen. At the same time, It could also resist to high temperature [15]. Thus, its quantity was higher in the alkaline environment, with high ammonium nitrogen concentration at the early stage of composting.…”

Section: Resultsmentioning

confidence: 99%

The succession pattern of bacterial diversity in compost using pig manure mixed with wood chips analyzed by 16S rRNA gene analysis

Yang

Xia

et al. 2019

Preprint

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26The pig manure mixed with wood chips and formed compost by means of 27 fermentation. We found that the protease activity, organic matter content and 28 ammonium nitrogen concentration were higher in the early stage of composting. 29 Meanwhile, the urease activity was highest in the high temperature period. The carbon 30 to nitrogen ratio of the compost decreased continuously with fermentation. The 31 dynamic change in the composition of bacterial overtime in the compost of a 180 kg 32 piles were explored using microbial diversity analysis. The results showed that the 33 microbial species increased with the compost fermentation. At the early stage of 34 composting, the phyla of Firmicutes and Actinomycetes were dominant. The microbes 35 in the high temperature period were mainly composed of Firmicutes and 36 Proteobacteria while the proportion of Bacteroides was increased during the cooling 37 period. In the compost of maturity stage, the proportion of Chloroflexi increased, 38 becoming dominant species with other microorganisms including Firmicutes, 39 Proteobacteria, Bacteroides, Chloroflexi but not Actinomycetes. Bacteria involved in 40 lignocellulose degradation, such as those of the Thermobifida, Cellvibrio, 41 Mycobacterium, Streptomyces and Rhodococcus, were concentrated in the maturity 42 stages of composting. Through correlation analysis, the environmental factors 43 including organic matter, ammonium nitrogen and temperature were consistent with 44 the succession of microbial including Rhodocyclaceae, Anaerolineaceae, 45 Thiopseudomonas, Sinibacillus and Tepidimicrobium. The change of urease activity 46 and carbon to nitrogen ratio corresponded to microbial communities, mainly 48 Corynebacterium, Bacillus, Anaerococcus, Lactobacillus, Ignatzschineria, and 49 Bacillaceae. 50 51 52 Aerobic composting of livestock manure and agricultural waste is the most 53 economical and environmentally friendly way of obtaining a fertilizer. During this 54 process, most microbes grow under aerobic conditions. Compared with anaerobic 55 fermentation, the aerobic fermentation cycle is shorter. The resulting composts can be 56 used in farmland as biological organic fertilizers, which is of great significance for 57 promoting ecological agriculture. 58 Aerobic composting generally undergoes four stages, including the heating period, 59 high temperature period, cooling period and maturity period Although the 60 microorganism communities of compost are highly complex, the succession of 61 microbial communities during composting obeys certain rules [1]. The traditional 62 research methods analyzing the microorganisms composition in compost mainly 63 include PLFA, DGGE, PCR-RFLP and plate culture method, etc.[2] However, 64 because of the limitations of culture conditions and the low resolution of 65 electrophoresis gels, these analyses of microorganisms have not been comprehensive.66Currently, the 16S RNA special fragment is amplified using the bacterial and archaeal 67 primers. By com...

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Section: Resultsmentioning

confidence: 99%

The succession pattern of bacterial diversity in compost using pig manure mixed with wood chips analyzed by 16S rRNA gene analysis

Yang

Xia

et al. 2019

Preprint

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“…Thiopseudomonas genus was found only in the anode of the terracotta-MFCs, BM (1.6%) and SM (4.2%). Facultative anaerobic Thiopseudomonas species were found oxidizing sulfide anaerobically with nitrate as electron acceptor in the sludge of an anaerobic, denitrifying, sulfide-removal bioreactor [47]. Several species of Clostridia were previously identified as electrogenic microorganisms [2,48].…”

Section: Figurementioning

confidence: 99%

A study of microbial communities on terracotta separator and on biocathode of air breathing microbial fuel cells

Rago¹,

Zecchin

Marzorati³

et al. 2018

Bioelectrochemistry

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Recently, terracotta has attracted interest as low-cost and biocompatible material to build separators in microbial fuel cells (MFCs). However, the influence of a non-conductive material like terracotta on electroactive microbiological communities remains substantially unexplored. This study aims at describing the microbial pools developed from two different seed inocula (bovine and swine sewage) in terracotta-based air-breathing MFC. A statistical approach on microbiological data confirmed different community enrichment in the MFCs, depending mainly on the inoculum. Terracotta separators impeded the growth of electroactive communities in contact with cathodes (biocathodes), while a thick biofilm was observed on the surface (anolyte-side) of the terracotta separator. Terracotta-free MFCs, set as control experiments, showed a well-developed biocathode, Biocathode-MFCs resulted in 4 to 6-fold higher power densities. All biofilms were analyzed by high-throughput Illumina sequencing applied to 16S rRNA gene. The results showed more abundant (3- to 5-fold) electroactive genera (mainly Geobacter, Pseudomonas, Desulfuromonas and Clostridia MBA03) in terracotta-free biocathodes. Nevertheless, terracotta separators induced only slight changes in anodic microbial communities.

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“…These data suggest a genome-wide alteration in GC content along with genome reduction in the evolution of P. caeni. Two genera of free-living bacteria related to P. caeni also have small genomes: Oblitimonas(Drobish et al, 2016) and Thiopseudomonas(Tan et al, 2015). Like P. caeni, strains of Oblitimonas alkaliphila have small genomes (2.2-2.5 Mb) and with average GC content of 47%-48%.…”

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confidence: 99%

Genome‐based evolutionary history of Pseudomonas spp

Hesse

Schulz

Bull

et al. 2018

Environmental Microbiology

172

253

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Pseudomonas is a large and diverse genus of Gammaproteobacteria. To provide a framework for discovery of evolutionary and taxonomic relationships of these bacteria, we compared the genomes of type strains of 163 species and 3 additional subspecies of Pseudomonas, including 118 genomes sequenced herein. A maximum likelihood phylogeny of the 166 type strains based on protein sequences of 100 single-copy orthologous genes revealed thirteen groups of Pseudomonas, composed of two to sixty three species each. Pairwise average nucleotide identities and alignment fractions were calculated for the data set of the 166 type strains and 1224 genomes of Pseudomonas available in public databases. Results revealed that 394 of the 1224 genomes were distinct from any type strain, suggesting that the type strains represent only a fraction of the genomic diversity of the genus. The core genome of Pseudomonas was determined to contain 794 genes conferring primarily housekeeping functions. The results of this study provide a phylogenetic framework for future studies aiming to resolve the classification and phylogenetic relationships, identify new gene functions and phenotypes, and explore the ecological and metabolic potential of the Pseudomonas spp.

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Thiopseudomonas denitrificans gen. nov., sp. nov., isolated from anaerobic activated sludge

Cited by 50 publications

References 22 publications

The succession pattern of bacterial diversity in compost using pig manure mixed with wood chips analyzed by 16S rRNA gene analysis

The succession pattern of bacterial diversity in compost using pig manure mixed with wood chips analyzed by 16S rRNA gene analysis

A study of microbial communities on terracotta separator and on biocathode of air breathing microbial fuel cells

Genome‐based evolutionary history of Pseudomonas spp

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