Thiols as major determinants of the total antioxidant capacity

Balcerczyk, Aneta; Grzelak, Agnieszka; Janaszewska, Anna; Jakubowski, Witold; Koziol, Sabina; Marszałek, Małgorzata; Rychlik, Błażej; Soszyński, Mirosław; Biliński, T; Bartosz, Grzegorz

doi:10.1002/biof.5520170108

Cited by 39 publications

(28 citation statements)

References 18 publications

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“…This is especially evident in yeast cells, where strains deficient in antioxidant enzymes show increased values of TAC due mainly to thiol-independent mechanisms. Similarly, the adaptation of yeast to conditions of stationary culture and reoxygenation after growth under anoxia predominantly involves antioxidants other than thiols, as demonstrated by Balcerczyk et al 49 .…”

Section: Acknowledgmentsmentioning

confidence: 87%

“…Balcerczyk et al 49 demonstrated that the TAC of cell extracts of S. cerevisiae showed a stronger dependence on the thiol content as evidenced by the effect of -SH blocking with n-ethylmaleimide (NEM). TAC measured after 10 s was decreased by 83-90% (in different strains) following thiol modification, while TAC measured after a 1-min reduction of ABTS* + was decreased by 73-80%.…”

Section: Acknowledgmentsmentioning

confidence: 99%

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Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Abegg

Alabarse

Schüller

et al. 2012

Rev. Soc. Bras. Med. Trop.

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Candida albicans, C. glabrata, C. tropicalis, and C. parapsilosis account for approximately 95% of identifiable Candida infections. Other species, including C. krusei, C. lusitaniae, and C. guilliermondii, account for less than 5% of cases of invasive candidiasis. The most common causative agent is still C. albicans, but its incidence is declining and the frequencies of other species are increasing. Recently, Furlaneto et al. 1 noted that non-albicans Candida was the predominant species in different clinical specimens, with the exception of urine samples, in a Brazilian tertiary-care hospital. Invasive candidiasis has a mortality rate that approaches 40% 2,3 . Although most people are colonized by Candida sp., the majority never develop invasive candidiasis. Alterations in host immunity, physiological features, or normal microflora, rather than the acquisition of novel or hypervirulent factors by Candida, are suggested to degenerate the commensal-host interaction and lead to an opportunistic infection 4 .During the course of a systemic infection, Candida cells are engulfed by host phagocytes, where they are exposed to reactive oxygen species (ROS) 5 . ROS contribute to the killing of C. albicans in both cultured cells and entire organisms [6][7][8][9] . Upon incubation with macrophages, C. albicans deoxyribonucleic acid (DNA) repair genes are transcriptionally induced, suggesting that DNA damage indeed occurs in the phagosome and that genotoxic hypersensitivity stress would be disadvantageous to the pathogen 10 . Recently, it was demonstrated that a large proportion of C. albicans cell surface antigens related to acute candidemia are involved in oxidative stress 4 . In C. albicans, hyphal cells ABSTRACT Introduction: The capacity to overcome the oxidative stress imposed by phagocytes seems to be critical for Candida species to cause invasive candidiasis. Methods: To better characterize the oxidative stress response (OSR) of 8 clinically relevant Candida sp., glutathione, a vital component of the intracellular redox balance, was measured using the 5,5'-dithiobis-(2-nitrobenzoic acid (DTNB)-glutathione disulfide (GSSG) reductase reconversion method; the total antioxidant capacity (TAC) was measured using a modified method based on the decolorization of the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic) acid radical cation (ABTS* + ). Both methods were used with cellular Candida sp. extracts treated or not with hydrogen peroxide (0.5 mM). Results: Oxidative stress induced by hydrogen peroxide clearly reduced intracellular glutathione levels. This depletion was stronger in Candida albicans and the levels of glutathione in untreated cells were also higher in this species. The TAC demonstrated intra-specific variation. Conclusions: Glutathione levels did not correlate with the measured TAC values, despite this being the most important non-enzymatic intracellular antioxidant molecule. The results indicate that the isolated measurement of TAC does not give a clear picture of the ability of a given Candida sp. to resp...

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Section: Acknowledgmentsmentioning

confidence: 87%

Section: Acknowledgmentsmentioning

confidence: 99%

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Abegg

Alabarse

Schüller

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…The progressive elevation of circulating concentrations of uric acid with age is well documented [22,23]. As urate and total thiols represent two major parameters of total radical-trapping capacity of human plasma [20,21,24], their differential modulations in pres-ence of cardiovascular disease is consistent with normal values of the biomarker of oxidative DNA damage (8-OHdG), suggesting a compensatory effect.…”

Section: Discussionmentioning

confidence: 89%

“…This defective pathway is usually a characteristic of cardiovascular pathologies [19]. Indeed, total SH groups critically contribute to the total antioxidative capacity of plasma measured using in vitro assays [20,21]. In fine, plasma concentrations of urate are significantly increased in subjects with cardiovascular disease.…”

Section: Discussionmentioning

confidence: 99%

Distinct profiles of systemic biomarkers of oxidative stress in chronic human pathologies: Cardiovascular, psychiatric, neurodegenerative, rheumatic, infectious, neoplasmic and endocrinological diseases

Brack¹,

Brack²,

Ménézo³

et al. 2013

ABB

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Oxidative stress is involved in chronic and acute pathologies: cardiovascular, neurodegenerative, neoplastic, inflammatory and infectious diseases. Clinical trials focused on prevention of cardiovascular and neoplastic diseases involving antioxidant supplementation have however provided predominantly negative obserations in large-scale studies. Screening of patient cohorts to assess baseline oxidative stress on the basis of a biomarker profile is decisive but lacking. For the first time, we evaluated the level of oxidative stress, testing more than 10 established biomarkers, in a comprehensive initial survey of 617 patients displaying chronic human pathologies. Multiple diseasespecific abnormalities were identified in plasma, whole blood and/or urine. This is the case for vitamins and oligo elements, vitamin C, vitamin E, β-carotene, selenium, zinc and copper; endogenous antioxidants such as reduced and oxidised glutathione, thiols, urate, and glutathione peroxidase activity, and a biomarker of oxidative DNA damage (8-hydroxy-2'-deoxyguanosine). The distinct biomarker profiles suggest the involvment of multiple forms of oxidative insults which are in some way partially specific to each pathological condition. This finding is in favor of the determination of an integrated score to combine contributions of distinct biomarkers, in order to screen patients presenting elevated levels of oxidative stress.

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“…2 Therefore, the total antioxidant system (TAS) and total oxidant system (TOS), along with thiol-disulphide regulation, is of key significance. Recent studies have indicated that abnormal thiol-disulphide balance is involved in the origin of AIDS, Alzheimer's disease, amyotrophic lateral sclerosis (ALS), cancer, cardiovascular disease, chronic kidney disease, diabetes, Friedreich's ataxia, multiple sclerosis, Parkinson's disease, and rheumatoid arthritis.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of the oxidative stress indices of children with autism and healthy children

Uğur¹,

Tunca²,

Sekmen³

et al. 2017

Anadolu Psikiyatri Derg

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Thiols as major determinants of the total antioxidant capacity

Cited by 39 publications

References 18 publications

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Distinct profiles of systemic biomarkers of oxidative stress in chronic human pathologies: Cardiovascular, psychiatric, neurodegenerative, rheumatic, infectious, neoplasmic and endocrinological diseases

A comparative study of the oxidative stress indices of children with autism and healthy children

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