Thiolated Polymers: Pharmaceutical Tool in Nasal Drug Delivery of Proteins and Peptides

Jain, Ashish; Hurkat, Pooja; Jain, Ankit; Jain, Abhishek Kumar; Jain, Sanjay

doi:10.1007/s10989-018-9704-y

Cited by 22 publications

(10 citation statements)

References 72 publications

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“…The antimicrobial effect of Ag+ ions has been well documented and it has been applied [78,79]. We are interested to investigate the potential of antibacterial activity of biologically synthesized AgNPs against H. pylori and H. felis.…”

Section: Dose-dependent Antibacterial Effects Of Agnpsmentioning

confidence: 99%

Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

Gurunathan¹,

Jeong²,

Han³

et al. 2016

Nano Online

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Silver nanoparticles (AgNPs) are prominent group of nanomaterials and are recognized for their diverse applications in various health sectors. This study aimed to synthesize the AgNPs using the leaf extract of Artemisia princeps as a bio-reductant. Furthermore, we evaluated the multidimensional effect of the biologically synthesized AgNPs in Helicobacter pylori , Helicobacter felis , and human lung (L132) and lung carcinoma (A549) cells. UV-visible (UV-vis) spectroscopy confirmed the synthesis of AgNPs. X-ray diffraction (XRD) indicated that the AgNPs are specifically indexed to a crystal structure. The results from Fourier transform infrared spectroscopy (FTIR) indicate that biomolecules are involved in the synthesis and stabilization of AgNPs. Dynamic light scattering (DLS) studies showed the average size distribution of the particle between 10 and 40 nm, and transmission electron microscopy (TEM) confirmed that the AgNPs were significantly well separated and spherical with an average size of 20 nm. AgNPs caused dose-dependent decrease in cell viability and biofilm formation and increase in reactive oxygen species (ROS) generation and DNA fragmentation in H. pylori and H. felis. Furthermore, AgNPs induced mitochondrial-mediated apoptosis in A549 cells; conversely, AgNPs had no significant effects on L132 cells. The results from this study suggest that AgNPs could cause cell-specific apoptosis in mammalian cells. Our findings demonstrate that this environmentally friendly method for the synthesis of AgNPs and that the prepared AgNPs have multidimensional effects such as anti-bacterial and anti-biofilm activity against H. pylori and H. felis and also cytotoxic effects against human cancer cells. This report describes comprehensively the effects of AgNPs on bacteria and mammalian cells. We believe that biologically synthesized AgNPs will open a new avenue towards various biotechnological and biomedical applications in the near future.

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Section: Dose-dependent Antibacterial Effects Of Agnpsmentioning

confidence: 99%

Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

Gurunathan¹,

Jeong²,

Han³

et al. 2016

Nano Online

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“…Many studies have demonstrated the importance of electrostatic interactions in mucoadhesion [9,19,43,44]. Additionally, mucoadhesive nanoparticle drug delivery systems containing thiol groups have resulted in increased retention because of the propensity for disulfide bond formation with mucins containing thiol groups [44,45]. Therefore, we hypothesized that S2E8K, S2E8R, S2E8E, and S2E8C may have favorable chemistries to facilitate retention of the nanoparticles in the mucosal membrane.…”

Section: Evaluation Of Selp Nanoparticle Mucoadhesive Propertiesmentioning

confidence: 99%

“…As previously described, SELPs containing arginine (R), lysine (K), glutamic acid (E), and cysteine (C) substitutes were selected for investigation as possible designs for mucoadhesive delivery systems, as these proteins contain charged groups and thiol groups resulting from the selected modification of the elastin "X" amino acid. Many studies have demonstrated the importance of electrostatic interactions in mucoadhesion [9,19,43,44]. Additionally, mucoadhesive nanoparticle drug delivery systems containing thiol groups have resulted in increased retention because of the propensity for disulfide bond formation with mucins containing thiol groups [44,45].…”

Section: Evaluation Of Selp Nanoparticle Mucoadhesive Propertiesmentioning

confidence: 99%

Design of Silk-Elastin-Like Protein Nanoparticle Systems with Mucoadhesive Properties

Parker

McKay

et al. 2019

JFB

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Transmucosal drug delivery is a promising avenue to improve therapeutic efficacy through localized therapeutic administration. Drug delivery systems that increase retention in the mucosal layer are needed to improve efficiency of such transmucosal platforms. However, the applicability of such systems is often limited by the range of chemistries and properties that can be achieved. Here we present the design and implementation of silk-elastin-like proteins (SELPs) with mucoadhesive properties. SELP-based micellar-like nanoparticles provide a system to tailor chemical and physical properties through genetic engineering of the SELP sequence, which enables the fabrication of nanoparticles with specific chemical and physical features. Analysis of the adhesion of four different SELP-based nanoparticle systems in an artificial mucus system, as well as in in vitro cellular assays indicates that addition of mucoadhesive chemical features on the SELP systems increases retention of the particles in mucosal environments. The results indicated that SELP-based nanoparticles provide a useful approach to study and develop transmucosal protein drug delivery system with unique mucoadhesive properties. Future studies will serve to further expand the range of achievable properties, as well as the utilization of SELPs to fabricate mucoadhesive materials for in vivo testing.

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“…The green area for the squamous mucosa, the blue area for the respiratory mucosa, and the yellow area for the olfactory mucosa (18).…”

Section: Figure (1): Different Regions Of Nasal Mucosamentioning

confidence: 99%

Nose to brain delivery of drugs for CNS diseases

Ali¹,

Alkotaji²

2021

IJP

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The management of central nervous system diseases is extremely challenging due to the numerous obstacles that stand against the successful delivery of drugs to their target site in the brain. Defeating the blood-brain barrier is considered the most significant challenge in this delivery. Different alternative routes of administration have been investigated. Nasal delivery is one of the possible ways for direct brain targeting. The nasal mucosa is the only part of the body at which the external environment become in straight connection with the central nervous system which takes place through the olfactory portion of the nasal mucosa. Different mechanisms have been suggested to describe the pathway for straight nasal to brain transport of medications, however, the precise route is still vague. The most important proposed pathways include nerve pathways (olfactory and trigeminal nerve), vascular, lymphatic, and cerebrospinal fluid mediated pathways. Among these mechanisms, the olfactory intra-neuronal delivery is the dominant one. Various particulate systems have been investigated for nasal delivery with the intention of direct transport to the brain. The most frequently studied delivery systems are polymeric nanosystems, lipid based nanosystems, and nanometric emulsions. In conclusion, direct nasal-to-brain delivery is a very fertile research area and any achievements in this area are running side by side with the progressions that occur in the particulate systems.

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Thiolated Polymers: Pharmaceutical Tool in Nasal Drug Delivery of Proteins and Peptides

Cited by 22 publications

References 72 publications

Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells

Design of Silk-Elastin-Like Protein Nanoparticle Systems with Mucoadhesive Properties

Nose to brain delivery of drugs for CNS diseases

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