Thioflavin T fluorescence in human serum: Correlations with vascular health and cardiovascular risk factors

Griffin, Michael D. W.; Wilson, Lewis; Mok, Yee-Foong; Januszewski, Andrzej S.; Wilson, Andrew; Karschimkus, Connie; Romas, E.; Lee, Allan B; Godfrey, Tim; Wong, Melinda; Clemens, Laurence; Jenkins, Alicia J.; Howlett, Geoffrey J.

doi:10.1016/j.clinbiochem.2009.10.010

Cited by 9 publications

(12 citation statements)

References 24 publications

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“…ThT has been used to predict cardiovascular disease 9 . In the literature is described a shift in the absorption band of ThT in the presence of amyloid structure 8 , but in this study this shift did not occur.…”

Section: Discussionmentioning

confidence: 99%

New blood markers for staging and prognostics of atherosclerosis

Sicchieri

Silva

Monteiro³

et al. 2014

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

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Analysis from the plasmas of rabbits subjected to high-cholesterol diets were performed by fluorescence spectroscopy using three biossensors: Europium-Chlortetracycline (EuCTc), Evans Blue (EB), and Thioflavin T (ThT). For this purpose an animal experimentation was done with New Zealand rabbits divided into two groups: control group of 6 rabbits that received a regular diet for 60 days; and experimental group of 9 rabbits, that were fed with 1% cholesterol for 60 days. The results from spectroscopic analysis have shown that the EuCTc marker emission intensity increases in the presence of plaque formation. The EB emission intensity remained constant for control and experimental groups. The ThT presented an increase in the emission intensity and a modification in the spectra shape with 60 days of diet. The studied biomarkers may not yet be specific in the identification of unstable plaques, but can provide additional information on the patients risk for plaque formation.

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Section: Discussionmentioning

confidence: 99%

New blood markers for staging and prognostics of atherosclerosis

Sicchieri

Silva

Monteiro³

et al. 2014

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

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“…Griffin e colaboradores [11] mostraram um aumento na emissão fluorescente ThT na presença de soro humano, demonstrando que a ThT pode ser um novo método para avaliação de risco cardiovascular.…”

Section: Tioflavina Tunclassified

“…Para estudar o comportamento do corante na presença das partículas de LDL os trabalhos também utilizam esse mesmo comprimento de onda de excitação [11], [16]. Portanto, nesse primeiro estudo da ThT na presença da LDL utilizou-se também 450 nm como comprimento de onda de excitação.…”

Section: Tioflavina Tunclassified

“…Desses novos marcadores pode-se citar o complexo Európio-Tetraciclinas (EuTcs) [10] e o corante benzotiazol Tioflavina T [11].…”

Section: Introductionunclassified

“…As partículas de LDL em seu estado oxidado formam fibrilas semelhantes às fibrilas de amiloidose [16]. Griffin e colaboradores mostraram que a Tioflavina T pode ser utilizada para marcar risco cardiovascular [11].…”

Section: Introductionunclassified

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