Thiodiketopiperazines Produced by Penicillium crustosum and Their Activities to Promote Gastrointestinal Motility

He, Xin; Yang, Jing; Qiu, Ling; Feng, Dacheng; Ju, Feng; Tan, Lu; Li, Yuzhi; Gu, Yu‐Cheng; Zhang, Zhen; Guo, Da‐Le; Deng, Yun

doi:10.3390/molecules24020299

Cited by 6 publications

(5 citation statements)

References 14 publications

(15 reference statements)

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“…Zebrafish culture and experimental procedure of gastrointestinal motility were performed as literature. [32] 4.6 Computational details…”

Section: Gastrointestinal Motility Of Zebrafishmentioning

confidence: 99%

Lignans from the Heartwood of Nothotsuga longibracteata

Liu¹,

Guo²,

Deng³

et al. 2020

Chin. J. Org. Chem.

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3-(4'-Hydroxy-3'-methoxybenzyl)-2(5H)-furanone (1), a rare benzyl furanone derivate, and two new lignans (2 and 3) were isolated from the heartwood of Nothotsuga longibracteata, together with nineteen known lignans compounds. The structures and absolute configurations of the undescribed compounds were elucidated on the basis of HR-ESI-MS, 1D NMR, 2D NMR, and CD spectroscopies. The cytotoxic effects of the isolated new lignan compounds on three human tumour cell lines (A172, SHSY5Y, and Hela) were evaluated by methyl thiazolyl tetrazolium (MTT) assay. Their showed no cytotoxic activities at the concentration of 50 μmol/L. Gastrointestinal motility test of zebrafish showed that compound 3 has the function of promoting gastrointestinal motility of zebrafish to excrete Nile red at doses 8 and 24 µmol/L by means of acting on the cholinergic nervous system.

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“…Zebrafish culture and experimental procedure of gastrointestinal motility were performed as literature. [32] 4.6 Computational details…”

Section: Gastrointestinal Motility Of Zebrafishmentioning

confidence: 99%

Lignans from the Heartwood of Nothotsuga longibracteata

Liu¹,

Guo²,

Deng³

et al. 2020

Chin. J. Org. Chem.

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“…Many difficulties in nomenclature derive from the stereostructural aspects. Concerning compounds 25-27, they were not named in the original manuscript [7]; however, a trivial name can be assigned according to their structurally related compound bilain B. However, the absence of a complete stereostructure determination prevents clarifying if compound 25 is actually its epimer or rather its diasteroisomer.…”

Section: Structures and Chemical Propertiesmentioning

confidence: 99%

“…In a quite peculiar assay carried out on zebrafish larvae, 1 and 6-oxo-methylthiosilvatin (12) promoted gastrointestinal motility via acting on the cholinergic nervous system, while bilains D-F (25)(26)(27) lacking the double bond in the lateral chain were inactive [7].…”

Section: Biological Activitiesmentioning

confidence: 99%

Occurrence and Properties of Thiosilvatins

et al. 2019

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The spread of studies on biodiversity in different environmental contexts is particularly fruitful for natural product discovery, with the finding of novel secondary metabolites and structural models, which are sometimes specific to certain organisms. Within the large class of the epipolythiodioxopiperazines, which are typical of fungi, thiosilvatins represent a homogeneous family that, so far, has been reported in low frequency in both marine and terrestrial contexts. However, recent observations indicate that these compounds have been possibly neglected in the metabolomic characterization of fungi, particularly from marine sources. Aspects concerning occurrence, bioactivities, structural, and biosynthetic properties of thiosilvatins are reviewed in this paper.

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“…Some groups use Nile Red to assay intestinal transit via dye clearing. [31][32][33][34][35] To fully leverage the fluorescent properties of Nile Red and better understand gut motility, we have developed an analysis method that detects contraction events based on periodic fluctuations in intraluminal fluorescence during gut wall movement. Propagating contraction events (pRCs, pACs, and aRCs) were tracked in the fluorescence time series as peaks or troughs against the median-subtracted signal, and the automated event detection algorithms that we developed retained all contraction event features such as amplitude, duration, and integrated area.…”

Section: Introductionmentioning

confidence: 99%

“…Nile Red dye selectively fluoresces in a hydrophobic environment, and when introduced to larval zebrafish it selectively labels lipid deposits in the intestines, gall bladder, and pancreas (for review 30 ). Some groups use Nile Red to assay intestinal transit via dye clearing 31–35 . To fully leverage the fluorescent properties of Nile Red and better understand gut motility, we have developed an analysis method that detects contraction events based on periodic fluctuations in intraluminal fluorescence during gut wall movement.…”

Section: Introductionmentioning

confidence: 99%

Systematic analysis of proximal midgut‐ and anorectal‐originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms

Cassidy,

Flores,

Trinh Nguyen

et al. 2023

Neurogastroenterology Motil

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BackgroundZebrafish larvae are translucent, allowing in vivo analysis of gut development and physiology, including gut motility. While recent progress has been made in measuring gut motility in larvae, challenges remain which can influence results, such as how data are interpreted, opportunities for technical user error, and inconsistencies in methods.MethodsTo overcome these challenges, we noninvasively introduced Nile Red fluorescent dye to fill the intraluminal gut space in zebrafish larvae and collected serial confocal microscopic images of gut fluorescence. We automated the detection of fluorescent‐contrasted contraction events against the median‐subtracted signal and compared it to manually annotated gut contraction events across anatomically defined gut regions. Supervised machine learning (multiple logistic regression) was then used to discriminate between true contraction events and noise. To demonstrate, we analyzed motility in larvae under control and reserpine‐treated conditions. We also used automated event detection analysis to compare unfed and fed larvae.Key ResultsAutomated analysis retained event features for proximal midgut‐originating retrograde and anterograde contractions and anorectal‐originating retrograde contractions. While manual annotation showed reserpine disrupted gut motility, machine learning only achieved equivalent contraction discrimination in controls and failed to accurately identify contractions after reserpine due to insufficient intraluminal fluorescence. Automated analysis also showed feeding had no effect on the frequency of anorectal‐originating contractions.Conclusions & InferencesAutomated event detection analysis rapidly and accurately annotated contraction events, including the previously neglected phenomenon of anorectal contractions. However, challenges remain to discriminate contraction events based on intraluminal fluorescence under treatment conditions that disrupt functional motility.

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Thiodiketopiperazines Produced by Penicillium crustosum and Their Activities to Promote Gastrointestinal Motility

Cited by 6 publications

References 14 publications

Lignans from the Heartwood of Nothotsuga longibracteata

Lignans from the Heartwood of Nothotsuga longibracteata

Occurrence and Properties of Thiosilvatins

Systematic analysis of proximal midgut‐ and anorectal‐originating contractions in larval zebrafish using event feature detection and supervised machine learning algorithms

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