Thiocyanate degradation by pure and mixed cultures of microorganisms

Souza-Fagundes, Elaine M.; Rosa, Luiz Henrique; Gomes, Newton C. M.; Santos, M.H.Silla; Pimentel, P.F.

doi:10.1590/s1517-83822004000300012

Cited by 11 publications

(7 citation statements)

References 14 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In soil, glucosinolates are hydrolysed by microorganisms (Fahey et al, 2001) and microbial degradation is the main mechanism for disappearance of isothiocyanates (Rumberger and Marschner, 2003). Microorganisms could perceive these metabolites as specific signal compounds stimulating growth but also use them as a nutritional substrate (Zeng et al, 2003;Souza-Fagundes et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The effects on fungal and bacterial responses of the glucosinolate-myrosinase defense system have already been described. However, these studies implicated pure strains of pathogen organisms and in vitro approaches (Brabban and Edwards, 1995;Kirkegaard et al, 1996;Smith and Kirkegaard, 2002;Souza-Fagundes et al, 2004). The incorporation of crucifer tissues showed a variety of effects on non-target soil microbial populations in studies based on isolation and culture of microorganisms (Scott and Knudsen, 1999;Bending and Lincoln, 2000;Cohen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

et al. 2009

View full text Add to dashboard Cite

A specificity of Brassicaceous plants is the production of sulphur secondary metabolites called glucosinolates that can be hydrolysed into glucose and biocidal products. Among them, isothiocyanates are toxic to a wide range of microorganisms and particularly soil-borne pathogens. The aim of this study was to investigate the role of glucosinolates and their breakdown products as a factor of selection on rhizosphere microbial community associated with living Brassicaceae. We used a DNA-stable isotope probing approach to focus on the active microbial populations involved in root exudates degradation in rhizosphere. A transgenic Arabidopsis thaliana line producing an exogenous glucosinolate and the associated wild-type plant associated were grown under an enriched 13 CO 2 atmosphere in natural soil. DNA from the rhizospheric soil was separated by density gradient centrifugation. Bacterial (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Acidobacteria), Archaea and fungal community structures were analysed by DGGE fingerprints of amplified 16S and 18S rRNA gene sequences. Specific populations were characterized by sequencing DGGE fragments. Roots of the transgenic plant line presented an altered profile of glucosinolates and other minor additional modifications. These modifications significantly influenced microbial community on roots and active populations in the rhizosphere. Alphaproteobacteria, particularly Rhizobiaceae, and fungal communities were mainly impacted by these Brassicaceous metabolites, in both structure and composition. Our results showed that even a minor modification in plant root could have important repercussions for soil microbial communities.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Thiocyanate (as free or metal-complex cyanides) in industrial waste waters represents a significant geoenvironmental risk due to the acute toxicity for living organisms [28]. However, as Au complexes with CN − (Equation (5); [27]) potential risks are negligible.…”

Section: Heap Leach Pile Evolutionmentioning

confidence: 99%

“…However, as Au complexes with CN − (Equation (5); [27]) potential risks are negligible. If thiocyanate species had complexed and persisted in these materials, exposure to atmospheric conditions for three decades would likely result in their breakdown [28] as per Equation (6) [29], with ammonia (NH3) eventually converted to nitrate (NO3). …”

Section: Heap Leach Pile Evolutionmentioning

confidence: 99%

Geoenvironmental Characterisation of Heap Leach Materials at Abandoned Mines: Croydon Au-Mines, QLD, Australia

Parbhakar-Fox

2016

Minerals

View full text Add to dashboard Cite

Abstract:Heap leaching is a well-established metallurgical technology which allows metal recovery (e.g., Au, Cu, U) from low-grade ores. However, spent heap leach materials remaining at abandoned or historic mine sites may represent a potential source of contamination. At the Croydon Au-mines, heap leaching operations (1984)(1985) were performed on mineralized rhyolites hosting sulphides including pyrite, galena, arsenopyrite and minor sphalerite. Characterization of spent heap leach materials (n = 14) was performed using established geochemical and mineralogical techniques, supplemented by automated mineralogical evaluations. Whilst these materials contained low sulphide-sulphur (0.08 to 0.41 wt %) and returned innocuous paste pH values (pH 5.1 to 8.6), they were classified uncertain by net acid producing potential/net acid generating criteria. This was likely due to the reaction of secondary mineral phases (i.e., beudantite, hidalgoite, kintoreite and Fe-As-Pb oxides) during these tests. It is hypothesised that during heap leaching, gangue sulphides have differentially reacted with the cyanide lixiviant, pre-conditioning the formation of these complex secondary phases during surficial oxidation, after heap leaching termination. These materials are considered to represent a moderate geoenvironmental risk as dissolved Pb in basal leachates is in excess of the World Health Organization (WHO 2006) guideline values. Considering this, these materials should be included in ongoing rehabilitation works at the site.

show abstract

“…Los productos finales fueron amonio y sulfato en una relación estequiométrica 1:1 (Kwon et al 2002). Actualmente se encuentran algunos reportes del uso de microorganismos para el tratamiento de efluentes mineros que contienen cianuro o tiocianatos como en Brasil a nivel de birreactores (Souza-Fagundes et al 2004 y a su vez la extracción de metales a partir de los minerales que los contienen.…”

Section: Introductionunclassified

Degradación de tiocianato por hongos aislados de ambientes mineros y evaluación de su capacidad degradativa

Medina¹,

Torres²,

Durán³

et al. 2012

Rev peru biol

View full text Add to dashboard Cite

used in gold production this compound is very toxic especially for environment, by nature reaction with sulfur it converts into thiocyanate (-SCN). In this paper, we isolate -SCN degrading fungus from Junin and Tumbes mining zones. Strains were assayed on 5 to 600 mM -SCN concentration, at least thiocyanate degrading kinetic was assayed at 1.2 g.L-1 of KSCN initial concentration, then the best strains had been morphological, biochemical and molecular identified using ITS RNA molecular marker. From 58 isolated fungus we found 4 unparfait funguses with a great thiocyanate degrading capacity those strains could be used for bioremediation processes. Thiocyanate degrading capacity of these strains was 50 g.L-1 on plate into 72 hours and their average capacity was 10.05 mg.L-1 -SCN with an standard velocity of -SCN degradation of 28.77 mg.L-1.h-1. Two strains were molecular identified as Fusarium trincictum by using ITS rDNA gene.

show abstract

Thiocyanate degradation by pure and mixed cultures of microorganisms

Cited by 11 publications

References 14 publications

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

Exogenous glucosinolate produced by Arabidopsis thaliana has an impact on microbes in the rhizosphere and plant roots

Geoenvironmental Characterisation of Heap Leach Materials at Abandoned Mines: Croydon Au-Mines, QLD, Australia

Degradación de tiocianato por hongos aislados de ambientes mineros y evaluación de su capacidad degradativa

Contact Info

Product

Resources

About