Thioacetalized coumarin-based fluorescent probe for mercury(II): ratiometric response, high selectivity and successful bioimaging application

A fluorescent probe for the dual detection of mercury ions and thiols based on a simple coumarin derivative

“…Thus the conclusion was reached that the E1 probe is useful for fluorogenic detection of Hg(ii) over a relatively wide pH range of pH 4-11. Changes in the fluorescence emission spectra of the E1 probe (20 µmol/L) upon addition of different amounts of Hg(ii) (0, 1,2,4,8,12,16,20,40 and 60 µmol/L) were measured ( Figure 5). It was found that with increasing amounts of Hg(ii) added to E1 that the intensity of the fluorescence emission band at 466 nm decreased gradually, and that the intensity of the emission peak at 395 nm increased.…”

“…However, the fluorescence emission spectra of the E1 probe changed in accordance with the nature of each amino acid which was added: Cys, Phe, Asn, Val, His, Glu and Gly all led to a decrease in intensity of the fluorescence emission band at 466 nm. The Changes in the fluorescence emission spectra of probe E1 (20 µmol/L) upon the addition of different amounts of Cys (0, 2,4,6,8,10,12,14,16,18,20 and 40 µmol/L) were measured ( Figure 8). The strongly fluorescent E1 probe gradually turned weakly fluorescent as increasing amounts of Cys were added to E1.…”

“…The development of selective and sensitive fluorescent probes for Hg(ii) is one of the best options for their detection. [17][18][19][20][21][22][23][24][25] Recently, efficient bifunctional fluorescent probes garnered substantial interest because they can be used for the simultaneous detection of two different analytes. For example, certain bifunctional fluorescent probes were capable of sensing Hg(ii) along with another analyte such as Ag(i), 26,27 Cu(ii), [28][29][30] Fe(iii), 31 Al(iii), 32 pH change, 33 saccharides 34 or F -.…”

A coumarin‐based fluorescent probe for Hg2+ and its application in living cells and zebrafish

“…[10][11][12][13] In recent years, some Hg fluorescent probes have been developed based on coumarin, BODIPY, rhodamine, and other dyes and applied for environmental and biological detection. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] However, most existing Hg 2+ fluorescent probes still have many shortcomings. For example, some fluorescent probes are not sufficiently sensitive, which severely limits their practical application for Hg detection.…”

First Report on the Synthesis of Antipyrine Crowned Siloxy Framework: Optical Recognition of Fe2+and Hg2+Ions

“…Methylated mercury (CH 3 Hg) augments its lipid solubility which is extremely toxic and can ruin the central nervous system. [4][5][6][7] As a consequence, World Health Organization (WHO) has fixed a stringent limit for mercury in drinking water which is 2μgL À 1 . [8] Considering the public health concerns, the introduction of simple and convenient sensors is needed for monitoring changes of heavy metal ions in the human body and environment.…”