Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives

Krujatz, Felix; Dani, Sophie; Windisch, Johannes; Emmermacher, Julia; Hahn, Franziska; Moßhammer, Maria; Murthy, Swathi; Steingroewer, Juliane; Walther, Thomas; Kühl, Michael; Gelinsky, Michael; Lode, Anja

doi:10.1016/j.biotechadv.2022.107930

Cited by 19 publications

(10 citation statements)

References 192 publications

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“…Finally, the presented 3D modelling approach can also be used to simulate different time-dependent environmental conditions (e.g., variable flow, day-night hypoxia, different solar irradiation regimes), which can help evaluating mechanisms driving coral stress responses as well as basic niche shaping factors for symbionts and microbiomes in the coral holobiont. The model could also be useful in more applied research such as in the ongoing attempts to create bionic corals (Wangpraseurt et al, 2022; Wangpraseurt et al, 2020) or for optimization of other 3D bioprinted constructs (Krujatz et al, 2022), where different designs can be evaluated and optimized. Our approach is also relevant for simulating structure-function relationships in other benthic systems such as photosynthetic biofilms and aquatic plant tissue, and can also be adapted to other sessile organisms such as symbiont-bearing giant clams, ascidians, jellyfish or foraminifera.…”

Section: Resultsmentioning

confidence: 99%

Modeling the radiative, thermal and chemical microenvironment of 3D scanned corals

Murthy

Picioreanu

Kühl

2023

Preprint

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1: Reef building corals are efficient biological collectors of solar radiation and consist of a thin stratified tissue layer spread over a light scattering calcium carbonate skeleton surface that together construct complex three dimensional (3D) colony structures forming the foundation of coral reefs. They exhibit a vast diversity of structural forms to maximize photosynthesis of their dinoflagellate endosymbionts (Symbiodiniaceae), while simultaneously minimizing photodamage. The symbiosis takes place in the presence of dynamic gradients of light, temperature and chemical species that are affected by the interaction of incident irradiance and water flow with the coral colony. 2: We developed a multiphysics modelling approach to simulate microscale spatial distribution of light, temperature and O2 in coral fragments with accurate morphology determined by 3D scanning techniques. 3: Model results compared well with spatial measurements of light, O2 and temperature under similar flow and light conditions. The model enabled us to infer the effect of coral morphology and light scattering in tissue and skeleton on the internal light environment experienced by the endosymbionts, as well as the combined contribution of light, water flow and ciliary movement on O2 and temperature distributions in the coral. 4: The multiphysics modeling approach is general enough to enable simulation of external and internal light, O2 and temperature microenvironments in 3D scanned coral species with varying degrees of branching and morphology under different environmental conditions. This approach is also relevant for simulating structure-function relationships in other benthic systems such as photosynthetic biofilms and aquatic plant tissue, and can also be adapted to other sessile organisms such as symbiont-bearing giant clams, ascidians, jellyfish or foraminifera. The model could also be useful in more applied research such as optimization of 3D bioprinted constructs where different designs can be evaluated and optimized.

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Section: Resultsmentioning

confidence: 99%

Modeling the radiative, thermal and chemical microenvironment of 3D scanned corals

Murthy

Picioreanu

Kühl

2023

Preprint

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“…In this study, the concept of cyanobacterial MICP was combined with the manufacturing method of 3D bioprinting, to investigate the possibility of producing biological cement (CaCO 3 ) in 3D printed constructs. Extrusion bioprinting with bioinks, hydrogels containing living cells, is an established technique especially in the biomedical and tissue engineering field ( Malda et al, 2013 ; Kilian et al, 2017 ; Valot et al, 2019 ; Ahlfeld et al, 2020 ), but there are also increasing applications in biotechnology ( Krujatz et al, 2022 ). Bioprinting like every AM technology allows the manufacturing of 3D constructs of predefined outer and inner morphology based on computer-aided design.…”

Section: Introductionmentioning

confidence: 99%

3D bioprinting of mineralizing cyanobacteria as novel approach for the fabrication of living building materials

Reinhardt

Ihmann

Ahlhelm

et al. 2023

Front. Bioeng. Biotechnol.

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Living building materials (LBM) are gaining interest in the field of sustainable alternative construction materials to reduce the significant impact of the construction industry on global CO2 emissions. This study investigated the process of three-dimensional bioprinting to create LBM incorporating the cyanobacterium Synechococcus sp. strain PCC 7002, which is capable of producing calcium carbonate (CaCO3) as a biocement. Rheology and printability of biomaterial inks based on alginate-methylcellulose hydrogels containing up to 50 wt% sea sand were examined. PCC 7002 was incorporated into the bioinks and cell viability and growth was characterized by fluorescence microscopy and chlorophyll extraction after the printing process. Biomineralization was induced in liquid culture and in the bioprinted LBM and observed by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and through mechanical characterization. Cell viability in the bioprinted scaffolds was confirmed over 14 days of cultivation, demonstrating that the cells were able to withstand shear stress and pressure during the extrusion process and remain viable in the immobilized state. CaCO3 mineralization of PCC 7002 was observed in both liquid culture and bioprinted LBM. In comparison to cell-free scaffolds, LBM containing live cyanobacteria had a higher compressive strength. Therefore, bioprinted LBM containing photosynthetically active, mineralizing microorganisms could be proved to be beneficial for designing environmentally friendly construction materials.

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“…Lastly, 3D bioprinting is not limited to mammalian or human cells, but can also make use of other cell types, e.g., microalgae or cyanobacteria. [ 27 ] These could be implemented across a variety of applications, such as bioreactors and life support systems (e.g., for oxygen production and waste treatment [ 28 ] ), as well as food and secondary metabolites production (e.g., vitamins). This topic has not been widely explored yet, but also from this angle, 3D bioprinting could be a very interesting technology for future deep space missions.…”

Section: Relevance Of Bioprinting For Applications In Spacementioning

confidence: 99%

3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space

Ombergen

Chalupa-Gantner

Chansoria

et al. 2023

Adv Healthcare Materials

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Abstract3D bioprinting has developed tremendously in the last couple of years and enables the fabrication of simple, as well as complex, tissue models. The international space agencies have recognized the unique opportunities of these technologies for manufacturing cell and tissue models for basic research in space, in particular for investigating the effects of microgravity and cosmic radiation on different types of human tissues. In addition, bioprinting is capable of producing clinically applicable tissue grafts, and its implementation in space therefore can support the autonomous medical treatment options for astronauts in future long term and far‐distant space missions. The article discusses opportunities but also challenges of operating different types of bioprinters under space conditions, mainly in microgravity. While some process steps, most of which involving the handling of liquids, are challenging under microgravity, this environment can help overcome problems such as cell sedimentation in low viscous bioinks. Hopefully, this publication will motivate more researchers to engage in the topic, with publicly available bioprinting opportunities becoming available at the International Space Station (ISS) in the imminent future.

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Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives

Cited by 19 publications

References 192 publications

Modeling the radiative, thermal and chemical microenvironment of 3D scanned corals

Modeling the radiative, thermal and chemical microenvironment of 3D scanned corals

3D bioprinting of mineralizing cyanobacteria as novel approach for the fabrication of living building materials

3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space

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