Thin nanoporous alumina-based SERS platform for single cell sensing

Toccafondi, Chiara; Rocca, Rosanna La; Scarpellini, Alice; Salerno, Marco; Das, Gobind; Dante, Silvia

doi:10.1016/j.apsusc.2015.05.169

Cited by 31 publications

(25 citation statements)

References 67 publications

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“…[166] Measurements were performed on living cells cultured on these SERS platforms, and the spectra were dominated by various components of the cells,f or example,a mino acids,p roteins,a nd phospholipids. Ar epresentative approach is based on using SERS-active surfaces on which the cells are located.…”

Section: Label-free Sers Detection Of Cells and Tissuesmentioning

confidence: 99%

“…Goldcoated nanoporous aluminum has been applied as SERSactive substrate to detect living cells. [166] Measurements were performed on living cells cultured on these SERS platforms, and the spectra were dominated by various components of the cells,f or example,a mino acids,p roteins,a nd phospholipids. As an alternative,p lasmonic paper-based SERS substrates were fabricated and used for the discrimination of cancer and normal cells.…”

Section: Label-free Sers Detection Of Cells and Tissuesmentioning

confidence: 99%

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Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and tissues by Raman microspectroscopy represents a nondestructive and label-free approach. All components of cells or tissues contribute to the Raman signals, giving rise to complex spectral signatures. Resonance Raman scattering and surface-enhanced Raman scattering can be used to enhance the signals and reduce the spectral complexity. Raman-active labels can be introduced to increase specificity and multimodality. In addition, nonlinear coherent Raman scattering methods offer higher sensitivities, which enable the rapid imaging of larger sampling areas. Finally, fiber-based imaging techniques pave the way towards in vivo applications of Raman spectroscopy. This Review summarizes the basic principles behind medical Raman imaging and its progress since 2012.

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Section: Label-free Sers Detection Of Cells and Tissuesmentioning

confidence: 99%

Section: Label-free Sers Detection Of Cells and Tissuesmentioning

confidence: 99%

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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“…After thoroughly rinsing with de-ionized water, blowing dry with nitrogen and dehydrating on a hotplate set at 100 °C for 15 min, the tAPA was overcoated by the same electron-beam evaporation system with a ≈25 nm thick Au layer to make it SERS-active. More details on similar fabrication procedure can be found in references [12–13]. …”

Section: Methodsmentioning

confidence: 99%

“…where P , t , A and I are laser power, accumulation time, active area for molecule adsorption and Raman intensity of the specific band, respectively [13]. The subscripts, SERS and Ref, indicate SERS and Raman measurements on tAPA–Au and on flat Au substrates, respectively.…”

Section: Methodsmentioning

confidence: 99%

Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina

Salerno

Shayganpour

Salis

et al. 2017

Beilstein J. Nanotechnol.

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Thin anodic porous alumina (tAPA) was fabricated from a 500 nm thick aluminum (Al) layer coated on silicon wafers, through single-step anodization performed in a Teflon electrochemical cell in 0.4 M aqueous phosphoric acid at 110 V. Post-fabrication etching in the same acid allowed obtaining tAPA surfaces with ≈160 nm pore diameter and ≈80 nm corresponding wall thickness to be prepared. The tAPA surfaces were made SERS-active by coating with a thin (≈25 nm) gold (Au) layer. The as obtained tAPA–Au substrates were incubated first with different thiols, namely mercaptobenzoic acid (MbA) and aminothiol (AT), and then with phospholipid vesicles of different composition to form a supported lipid bilayer (SLB). At each step, the SERS substrate functionality was assessed, demonstrating acceptable enhancement (≥100×). The chemisorption of thiols during the first step and the formation of SLB from the vesicles during the second step, were independently monitored by using a quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The SLB membranes represent a simplified model system of the living cells membranes, which makes the successful observation of SERS on these films promising in view of the use of tAPA–Au substrates as a platform for the development of surface-enhanced Raman spectroscopy (SERS) biosensors on living cells. In the future, these tAPA–Au-SLB substrates will be investigated also for drug delivery of bioactive agents from the APA pores.

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“…Due to the small thickness of the metal film, the anodization was carried out in a single step, yielding a fully oxidized layer in about 10 s. A similar protocol was used to fabricate tAPA, as described in [10,11]. In both cases, the process is self-terminating, because the current goes to zero as soon as the metal is completely oxidized.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of Gold-Coated Ultra-Thin Anodic Porous Alumina Substrates for Augmented SERS

et al. 2016

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Anodic porous alumina (APA) is a nanostructured material used as a template in several nanotechnological applications. We propose the use of APA in ultra-thin form (<100 nm) for augmented surface-enhanced Raman scattering (SERS). Here, the effect of in-depth thinning of the APA nanostructures for possible maximization of SERS was addressed. Anodization was carried out on ultra-thin films of aluminum on glass and/or silicon, followed by pore-opening. Gold (Au) was overcoated and micro-Raman/SERS measurements were carried out on test target analytes. Finite integration technique simulations of the APA-Au substrate were used both for the experimental design and simulations. It was observed that, under optimized conditions of APA and Au thickness, the SERS enhancement is higher than on standard APA-Au substrates based on thin (~100 nm) APA by up to a factor of ~20 for test molecules of mercaptobenzoic acid. The agreement between model and experimental results confirms the current understanding of SERS as being mainly due to the physical origin of plasmon resonances. The reported results represent one step towards micro-technological, integrated, disposable, high-sensitivity SERS chemical sensors and biosensors based on similar substrates.

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Thin nanoporous alumina-based SERS platform for single cell sensing

Cited by 31 publications

References 67 publications

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina

Fabrication of Gold-Coated Ultra-Thin Anodic Porous Alumina Substrates for Augmented SERS

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