Thin-layer MoS2 and thionin composite-based electrochemical sensing platform for rapid and sensitive detection of zearalenone in human biofluids

Jiang, Ke‐Jian; Nie, Dongxia; Huang, Qingwen; Fan, Kai; Tang, Zhanmin; Wu, Yongjiang; Han, Zheng

doi:10.1016/j.bios.2019.02.003

Cited by 52 publications

(14 citation statements)

References 45 publications

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“…For ZEA, some biosensors have also been investigated. Jiang et al (2019) [103] determined ZEA levels in plasma and urine samples collected from healthy volunteers using electrochemical immunosensors. ACN acidified with 1% formic acid was added to the samples to precipitate proteins.…”

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Arce-López

Lizarraga

Vettorazzi

et al. 2020

Toxins

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This manuscript reviews the state-of-the-art regarding human biological monitoring (HBM) of mycotoxins in plasma, serum and blood samples. After a comprehensive and systematic literature review, with a focus on the last five years, several aspects were analyzed and summarized: (a) the biomarkers analyzed and their encountered levels, (b) the analytical methodologies developed and (c) the relationship between biomarker levels and some illnesses. In the literature reviewed, aflatoxin B1-lysine (AFB1-lys) and ochratoxin A (OTA) in plasma and serum were the most widely studied mycotoxin biomarkers for HBM. Regarding analytical methodologies, a clear increase in the development of methods for the simultaneous determination of multiple mycotoxins has been observed. For this purpose, the use of liquid chromatography (LC) methodologies, especially when coupled with tandem mass spectrometry (MS/MS) or high resolution mass spectrometry (HRMS) has grown. A high percentage of the samples analyzed for OTA or aflatoxin B1 (mostly as AFB1-lys) in the reviewed papers were positive, demonstrating human exposure to mycotoxins. This review confirms the importance of mycotoxin human biomonitoring and highlights the important challenges that should be faced, such as the inclusion of other mycotoxins in HBM programs, the need to increase knowledge of mycotoxin metabolism and toxicokinetics, and the need for reference materials and new methodologies for treating samples. In addition, guidelines are required for analytical method validation, as well as equations to establish the relationship between human fluid levels and mycotoxin intake.

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Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Arce-López

Lizarraga

Vettorazzi

et al. 2020

Toxins

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“…The 2D nanomaterial-based electrochemical biosensors have also been developed for detecting other mycotoxins produced by Fusarium including deoxynivalenol (DON), fumonisin 1 (FB1), and zearalenone (ZEN) [132][133][134][135][136][137]. Shi et al developed an aptasensor for sensitive FB1 detection by using the dual amplification of AuNPs and graphene/thionine nanocomposites (GSTH) [132].…”

Section: Mycotoxins Produced By Fusariummentioning

confidence: 99%

“…The immunosensor can be used for simultaneous detection of multiple co-contaminant mycotoxins individually in the practical samples (e.g., corn extracts) because it shows low matrix interference even in co-existing toxin environments. Very recently, Jiang et al constructed a facile electrochemical immunosensor based on thin-layer MoS 2 and thionin (MoS 2 -Thi) composites for the sensitive and rapid detection of zearalenone (ZEA) in human biofluids (as shown in Figure 7) [136]. The as-prepared MoS 2 -Thi nanocomposites were employed as excellent electrochemical probes, as well as an efficient anti-ZEA antibody loading platform because MoS 2 retains the electrochemical activity of Thi, and has a large surface area.…”

Section: Mycotoxins Produced By Fusariummentioning

confidence: 99%

“…The MoS 2 -Thi-based electrochemical immunosensor has good ZEA detection performance including a wide linear range (0.01 to 50 ng mL −1 ), low LOD (0.005 ng mL −1 ZEA in both the plasma and urine), excellent selectivity, rapid responding time (20 min), acceptable stability (retained more than 85% detection capability at 4 • C for 10 days) and good practicability (detection of ZEA in real human biofluids). Figure 7) [136]. The as-prepared MoS2-Thi nanocomposites were employed as excellent electrochemical probes, as well as an efficient anti-ZEA antibody loading platform because MoS2 retains the electrochemical activity of Thi, and has a large surface area.…”

Section: Mycotoxins Produced By Fusariummentioning

confidence: 99%

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Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Jian

et al. 2019

Toxins

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Toxin detection is an important issue in numerous fields, such as agriculture/food safety, environmental monitoring, and homeland security. During the past two decades, nanotechnology has been extensively used to develop various biosensors for achieving fast, sensitive, selective and on-site analysis of toxins. In particular, the two dimensional layered (2D) nanomaterials (such as graphene and transition metal dichalcogenides (TMDs)) and their nanocomposites have been employed as label and/or biosensing transducers to construct electrochemical biosensors for cost-effective detection of toxins with high sensitivity and specificity. This is because the 2D nanomaterials have good electrical conductivity and a large surface area with plenty of active groups for conjugating 2D nanomaterials with the antibodies and/or aptamers of the targeted toxins. Herein, we summarize recent developments in the application of 2D nanomaterial-based electrochemical biosensors for detecting toxins with a particular focus on microbial toxins including bacterial toxins, fungal toxins and algal toxins. The integration of 2D nanomaterials with some existing antibody/aptamer technologies into electrochemical biosensors has led to an unprecedented impact on improving the assaying performance of microbial toxins, and has shown great promise in public health and environmental protection.

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“…However, these methods have exhibited some drawbacks such as time‐consuming in sample detection, complex and expensive instruments required, and complicated sample pretreatments needed. Recently, electrochemical detection methods have attracted considerable interest because they are relatively more facile and stable, less time‐consuming, more environment‐friendly and less costly besides the excellent detection limits . Previously reported studies have indicated that the fabrication of modified electrodes is essential for electrochemical analysis .…”

Section: Introductionmentioning

confidence: 99%

Differential Pulse Voltammetry Determination of Ofloxacin in Human Serum and Urine Based on a Novel Tryptophan‐graphene Oxide‐carbon Nanotube Electrochemical Sensor

Zhu

Chen

et al. 2019

Electroanalysis

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The highly sensitive determination of ofloxacin (OFL) in human serum and urine was achieved on a novel tryptophan‐graphene oxide‐carbon nanotube (Trp‐GO‐CNT) composite modified glassy carbon electrode (Trp‐GO‐CNT/GCE). The Trp‐GO‐CNT composite was fabricated, and its morphologies and surface functional groups were characterized by field emission scanning electron microscopy (FE‐SEM) and Fourier transform infrared (FT‐IR) spectroscopy. The electrochemical properties of Trp‐GO‐CNT/GCE were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The superior electrochemical behaviors of Trp‐GO‐CNT/GCE toward OFL can be mainly assigned to the excellent electrocatalytic activity of Trp, the great conductivity and high surface area of GO and CNT, and the synergistic effect between Trp, GO and CNT. Under optimum conditions, a wide and valuable linear range (0.01–100 μM), a low detection limit (0.001 μM, S/N=3), a good linear relationship (R2>0.999), good stability and repeatability were obtained for the quantitative determination of OFL. Furthermore, the Trp‐GO‐CNT electrochemical sensor was successfully applied to the determination of OFL in human serum and urine samples, and satisfactory accuracy and recovery could be obtained.

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Thin-layer MoS2 and thionin composite-based electrochemical sensing platform for rapid and sensitive detection of zearalenone in human biofluids

Cited by 52 publications

References 45 publications

Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Differential Pulse Voltammetry Determination of Ofloxacin in Human Serum and Urine Based on a Novel Tryptophan‐graphene Oxide‐carbon Nanotube Electrochemical Sensor

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