Thin layer immunoassay; an economical approach to diagnoseHelicobacter pyloriInfection in gastroduodenal ulcer disease patients of Pakistan; a comparative analysis

Abstract

Helicobacter pylori is a causative agent of gastritis, gastroduodenal ulcers and gastric adenocarcinoma. Gastric patient's serums were screened for H. pylori infection by thin layer immunoassay. A polystyrene plate coated with H. pylori sonicate whole cell antigen (10 µg/ml). 2 Two fold-diluted patient's serum was allowed to react at 37 ºC, incubated at 60 ºC for 1 min over water bath and recorded water condensation pattern for H. pylori antibody. Gastric patient's blood samples (62% male and 6% female) were tested positive for H. pylori, while agewise 15-25 years males (36%) and 65-75 years females (50%) showed highest number of H. pylori infection. Thin layer immunoassay showed sensitivity (72-67%), specificity (100%), accuracy (94-69%) and κ value (0.493-0.357) in comparison with wELISA, sELISA and kELISA. We conclude thin layer immunoassay was reliable, low cost, quick, simple and clinically useful method for H. pylori diagnosis in patients of Pakistan.