Thin‐Layer Gel Filtration Immunoelectrophoresis

Abstract

A method that directly combines separation of proteins by thin‐layer gel filtration (TLG) with quantitative immunoelectrophoresis is described. All the operations are performed in monolayer gels on a single glass plate. The resolving power of the method is comparable to that of an ordinary column technique monitored by fused rocket immunoelectrophoresis.

Enzyme Characterization in Quantitative Immunoelectrophoresis

“…Quantitative immunoelectrophoresis followed by enzymatic identification reactions has been used to study different enzymes: serum cholinesterase (21,22,23,26,44,60,84), acetylcholinesterase from erythrocyte membrane (16), other esterases (14, 17-19, 27, 28, 58, 69), acid phosphatases (14, 17-19, 26, 57, 5 8 , 69), nucleoside diphosphatases and triphosphatases (14,(17)(18)(19)69), u-amylases (29,46), amino-peptidases (14, 17-19, 46, 69), and other enzymes (14, 17-19, 34, 58, 69).…”