Thin‐layer chromatography separation of enantiomers of verapamil using macrocyclic antibiotic as a chiral selector

Bhushan, Ravi; Gupta, Divender

doi:10.1002/bmc.520

Cited by 30 publications

(12 citation statements)

References 36 publications

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“…In contrast, the extract stream was rich in R-(+)-verapamil, but was contaminated with the S-(-)-verapamil enantiomer. The [α] D at 25 ºC was determined for both enantiomers, and the values obtained were close to those previously reported (Bhushan and Gupta, 2005 (Figure 9 and 10).…”

Section: Results For Continuous Runs In the Varicol Systemsupporting

confidence: 89%

Chromatographic Separation of Verapamil Racemate Using a Varicol Continuous Multicolumn Process

Cremasco

Santana²

2015

Braz. J. Chem. Eng.

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-Verapamil is a chiral drug that is marketed in its racemic form, but because of the pharmacological effects due to molecule's chirality, one of the enantiomers is more potent, and the other exhibits different activities of therapeutic interest. The preparative separation of the verapamil enantiomers was performed using a continuous Varicol unit operated on a scale of 1 g/day. Amylose tris(3,5-dimethylphenylcarbamate) functioned as the stationary phase, and n-hexane/isopropanol/ethanol mixtures were used as the mobile phase. Diethylamine was used as the additive. The enantiomeric purities were 93.0% for S-(-)-verapamil and 92.0% for R-(+)-verapamil in the raffinate and extract streams, respectively. The unit provided productivities of 0.18 kg of raffinate per day per kg of adsorbent and 0.20 kg of extract per day per kg of adsorbent when using a feed concentration of 12.5 g L -1 .

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Section: Results For Continuous Runs In the Varicol Systemsupporting

confidence: 89%

Chromatographic Separation of Verapamil Racemate Using a Varicol Continuous Multicolumn Process

Cremasco

Santana²

2015

Braz. J. Chem. Eng.

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“…Calcium channel blockers have been determined by a variety of analytical techniques, such as HPLC [2][3][4][5][6][7][8][9][10], HPTLC [11][12][13][14][15][16], gas chromatography [17][18][19][20][21], Micellar electrokinetic chromatography [22][23][24][25], electroanalytical methods [26][27][28] and spectrophotometric methods [29][30][31][32][33][34].…”

Section: Amlodipine Besylate (Adb)mentioning

confidence: 99%

Spectrophotometric determination of some calcium channel blockers using Sulfochlorophenol-S, Bromopyrogallol red, Eriochromecyanine-R and Pyrocatechoil violet

Omar¹,

Abdelmageed²,

Abdelgaber³

et al. 2013

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“…12 In patients with hepatic insufficiency, metabolism is delayed and elimination half-life is prolonged up to 14-16 hours. These include spectrophotometry, 15 high performance liquid chromatography (HPLC) with spectrophotometric detection, [16][17][18][19][20][21] high performance thin layer chromatography, 22,23 and liquid chromatography-tandem mass spectrometry (LC-MS/MS). † These authors contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

Comparative pharmacokinetics of trandolapril, its active metabolite, and verapamil in human plasma of Egyptian population using HPLC–MS/MS

Magdy

El‐Khatib

Hemdan

et al. 2018

Drug Testing and Analysis

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Trandolapril and verapamil are commonly used antihypertensive drugs. However, there is a lack of available data on the change of their pharmacokinetics in patients with liver or kidney impairment and hence the need for dose adjustment. In this article, a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the monitoring of trandolapril, its active metabolite trandolaprilat, and verapamil in human plasma of patients with renal impairment and/or liver insufficiency. The chromatographic separation was achieved on a Gemini C reversed phase column using a gradient elution mode with a run time of 10 minutes. The mobile phase consisted of a mixture of methanol and 2% acetic acid. The electrospray ionization MS/MS analysis was performed in multiple reaction monitoring (MRM) mode. The assay was validated as per Food and Drug Administration (FDA) guidelines for bioanalytical method validation and proved to be suitable for the determination of therapeutic drug levels in plasma. The inter-group changes in pharmacokinetic data were compared to that of healthy volunteers. The comparison showed a significant difference in the pharmacokinetic parameters between the studied groups. The presented results exhibit the benefits of the proposed assay as a validated analytical tool for the continuous drug monitoring.

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Thin‐layer chromatography separation of enantiomers of verapamil using macrocyclic antibiotic as a chiral selector

Cited by 30 publications

References 36 publications

Chromatographic Separation of Verapamil Racemate Using a Varicol Continuous Multicolumn Process

Chromatographic Separation of Verapamil Racemate Using a Varicol Continuous Multicolumn Process

Spectrophotometric determination of some calcium channel blockers using Sulfochlorophenol-S, Bromopyrogallol red, Eriochromecyanine-R and Pyrocatechoil violet

Comparative pharmacokinetics of trandolapril, its active metabolite, and verapamil in human plasma of Egyptian population using HPLC–MS/MS

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