Thin-Layer Chromatography of Plant Pigments on Mannitol or Sucrose

Smith, L. W.; Breidenbach, R. W.; Rubenstein, Daniel Jay

doi:10.1126/science.148.3669.508

Cited by 25 publications

(5 citation statements)

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“…When fatty acid labeled Tween 20 is eluted through the Sephadex column, a portion of the label appears in the same fraction as the oxyethylene labeled material, but other fractions collected contain more or less radioactivity than similar fractions collected for the polyoxyethylene labeled material. Each of these fatty acid labeled fractions showed the same [10][11][12][13] different compounds when chromatographed on Whatman No. 1 paper and thin-layer medium (Figures 3 and 4), Thus, these Tween 20-C14 samples, by virtue of their manufacture, contain many compounds and together with the differences in chain length of the three polyoxyethylene side chains numerous homologs will exist.…”

Section: Discussionmentioning

confidence: 99%

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C¹⁴-Fatty Acid and Oxyethylene Labeled

Smith¹,

Foy²

1966

J. Agric. Food Chem.

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Evidence from column, paper, and thin-layer chromatography indicates that Tween 20 is a mixture of numerous compounds. Carbon-14 distribution patterns in bean, barley, and cotton were determined following foliar and root applications of Tween 20-C14 (oxyethyiene and fatty acid labeled), and of fractions of both materials eluted from a CM Sephadex column. Marked differences existed between the oxyethyiene and fatty acid labeled surfactants with respect to the distribution of C14 in the plants from either root or foliar application. The fatty acid label was principally phloem mobile, whereas the oxyethyiene label was mainly xylem mobile. The penetration and subsequent movement of label from either sample of Tween 20-C14 in all species studied were very low. No Tween 20-C14 was detected in the plant outside the treated area following either foliar or root application of fatty acid labeled surfactant; results with oxyethyiene

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Section: Discussionmentioning

confidence: 99%

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C¹⁴-Fatty Acid and Oxyethylene Labeled

Smith¹,

Foy²

1966

J. Agric. Food Chem.

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“…This system approximates that of GOUGH (6) for the separation of porphyrins and is used without equilibration. 2) Mannitol thin layer plates are run with a solvent of 0.5% propanol in petroleum ether (40 ~ 60~ (16). 3) For cellulose thin layer plates a solvent is used consisting of petroleum ether (40~176 benzene, acetone, 4:1:0.5, v/v/v, according to HOLDEN (8).…”

Section: Assay For Chlorophyll Synthesis By Isolated Plastidsmentioning

confidence: 99%

Synthesis of Δ-aminolevulinic acid and chlorophyll by isolated chloroplasts

Kannangara

Gough

1977

Carlsberg Res. Commun.

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Plastids isolated from immature spinach and greening barley or maize leaves incorporate L-glutamate-~4C or aketoglutarate-14C into b-aminolevulinate in the presence of levulinate. In its absence barley plastids incorporated L-glutamate-]4C into chlorophyll a. Optimal incorporation of label was obtained with plastids isolated and incubated in glycerol containing media. The half-life of the b-aminolevulinate forming activity of the plastids was 5 min at 22~ and 1 hour at 0~ In these plastid preparations b-aminolevulinate synthesis is stimulated by light, ATP and aerobic conditions. Experiments employing photosynthetic inhibitors and artificial electron donors indicate the light stimulation to be indirect by provision of ATP and reducing power. Aminooxyacetate, DL-13-hydroxyglutamate and glyoxylate inhibited the synthesis ofb-aminolevulinate. In precursor competition experiments with a-ketoglutarate, L-glutamate is preferentially incorporated into b-aminolevulinate. The plastids formed nmolar quantities of chemically identifiable b-aminolevulinate.

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“…However, tic on cellulose does not usually give a good separation of Chi a and b and of these two compounds from other pigments; the spots become very large and tail badly as they are eluted up the cellulose layer. Tic On sucrose has been described (Colman and Vishniac, 1964;Nutting et al, 1965;Smith et al, 1965), although these systems need improvement by elution selection. Our systems do give a very good separation.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of chlorophyll in dried bract of cotton plant

Loewenschuss¹,

Wakelyn²

1973

J. Agric. Food Chem.

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Research has been published which disclosed that the. proportions of palmitate decreased and oleate increased considerably in the PE and PC remaining after storage of orange juice at 85°F (Nagy and Nordby, 1970). They reported a loss of approximately 90% of the PE and PC during 85°F storage of orange juice. This means that the changes in palmitate and oleate were measured from fatty acid determinations on only the remaining 10% of PE and PC. Also, Nagy and Nordby showed in their Table III that the four major fatty acids in the free fatty acid fraction increased by 85-90% during storage at 85°F. These data do nót indicate any selective hydrolysis of the 90% of PE and PC reported as destroyed, but only that the 10% of unhydrolyzed PE and PC had less palmitate and more oleate than in the initial concentrations of these two phospholipids. These authors postulated that the changes in these two fatty acids were due to a specific action of phospholipases in the juice, resulting in complete hydrolysis of certain phospholipids, as no lyso-phospholipids were detected. LITERATURE CITED

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Thin-Layer Chromatography of Plant Pigments on Mannitol or Sucrose

Abstract: Fat-soluble tobacco leaf pigments were separated by chromatography on thin layers of mannitol and sucrose. Resolution of pigments, simplicity, and rapidity of preparation and development offer advantages over previous methods.

Cited by 25 publications

References 1 publication

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C¹⁴-Fatty Acid and Oxyethylene Labeled

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C¹⁴-Fatty Acid and Oxyethylene Labeled

Synthesis of Δ-aminolevulinic acid and chlorophyll by isolated chloroplasts

Occurrence of chlorophyll in dried bract of cotton plant

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Thin-Layer Chromatography of Plant Pigments on Mannitol or Sucrose

Abstract: Fat-soluble tobacco leaf pigments were separated by chromatography on thin layers of mannitol and sucrose. Resolution of pigments, simplicity, and rapidity of preparation and development offer advantages over previous methods.

Cited by 25 publications

References 1 publication

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C14-Fatty Acid and Oxyethylene Labeled

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C14-Fatty Acid and Oxyethylene Labeled

Synthesis of Δ-aminolevulinic acid and chlorophyll by isolated chloroplasts

Occurrence of chlorophyll in dried bract of cotton plant

Contact Info

Product

Resources

About

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C¹⁴-Fatty Acid and Oxyethylene Labeled

Herbicide Activator, Penetration and Distribution Studies in Bean, Cotton, and Barley from Foliar and Root Applications of Tween 20-C¹⁴-Fatty Acid and Oxyethylene Labeled