1965
DOI: 10.1126/science.148.3669.508
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Thin-Layer Chromatography of Plant Pigments on Mannitol or Sucrose

Abstract: Fat-soluble tobacco leaf pigments were separated by chromatography on thin layers of mannitol and sucrose. Resolution of pigments, simplicity, and rapidity of preparation and development offer advantages over previous methods.

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Cited by 25 publications
(5 citation statements)
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“…When fatty acid labeled Tween 20 is eluted through the Sephadex column, a portion of the label appears in the same fraction as the oxyethylene labeled material, but other fractions collected contain more or less radioactivity than similar fractions collected for the polyoxyethylene labeled material. Each of these fatty acid labeled fractions showed the same [10][11][12][13] different compounds when chromatographed on Whatman No. 1 paper and thin-layer medium (Figures 3 and 4), Thus, these Tween 20-C14 samples, by virtue of their manufacture, contain many compounds and together with the differences in chain length of the three polyoxyethylene side chains numerous homologs will exist.…”
Section: Discussionmentioning
confidence: 99%
“…When fatty acid labeled Tween 20 is eluted through the Sephadex column, a portion of the label appears in the same fraction as the oxyethylene labeled material, but other fractions collected contain more or less radioactivity than similar fractions collected for the polyoxyethylene labeled material. Each of these fatty acid labeled fractions showed the same [10][11][12][13] different compounds when chromatographed on Whatman No. 1 paper and thin-layer medium (Figures 3 and 4), Thus, these Tween 20-C14 samples, by virtue of their manufacture, contain many compounds and together with the differences in chain length of the three polyoxyethylene side chains numerous homologs will exist.…”
Section: Discussionmentioning
confidence: 99%
“…This system approximates that of GOUGH (6) for the separation of porphyrins and is used without equilibration. 2) Mannitol thin layer plates are run with a solvent of 0.5% propanol in petroleum ether (40 ~ 60~ (16). 3) For cellulose thin layer plates a solvent is used consisting of petroleum ether (40~176 benzene, acetone, 4:1:0.5, v/v/v, according to HOLDEN (8).…”
Section: Assay For Chlorophyll Synthesis By Isolated Plastidsmentioning
confidence: 99%
“…However, tic on cellulose does not usually give a good separation of Chi a and b and of these two compounds from other pigments; the spots become very large and tail badly as they are eluted up the cellulose layer. Tic On sucrose has been described (Colman and Vishniac, 1964;Nutting et al, 1965;Smith et al, 1965), although these systems need improvement by elution selection. Our systems do give a very good separation.…”
Section: Resultsmentioning
confidence: 99%