Thin-Layer Chromatography of Malto-oligosaccharides.

Weill, C. Edwin; Hanke, Paul

doi:10.1021/ac60193a024

Cited by 75 publications

(17 citation statements)

References 9 publications

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“…Stools were collected during the 24-hr period preceding and the 72-hr period following oral administration of the test substance: in each stool sample, pH [9], lactic acid [33], glucose, and starch [5] were determined. Carbohydrates in stool were separated and detected by thin layer chromatography [34] after treatment of feces with acetone [17]. Maltotriose was prepared in our laboratory according to the procedure described in the appendix to this paper.…”

Section: Methodsmentioning

confidence: 99%

Intraluminal and Mucosal Starch Digestion in Congenital Deficiency of Intestinal Sucrase and Isomaltase Activities

et al. 1972

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ExtractTen patients affected by congenital deficiency of intestinal sucrase and isomaltase activities were studied. The intraluminal a-amylolysis of amylopectin was found to be normal in these patients. It is incomplete during the 1st year of life, both in patients and in controls, as a consequence of the lower a-amylase activity in this age group.Maltotriose was well tolerated in two normal controls ages 32 and 3 months and in one 31-month-old patient: in these subjects an oral tolerance test with the trisaccharide was followed by a rapid increase of blood glucose (46-58 mg/100 ml) and did not cause diarrhea. However, in a 6-month-old patient fermentative diarrhea and low increase of blood glucose (24 mg/100 ml) followed oral load with maltotriose. In all patients, enzyme assays on intestinal biopsy specimens showed that the glucamylase activity of the heat-resistant maltase(s) was normal, while that of the sucrase-isomaltase complex was decreased.These results suggest that starch malabsorption in congenital sucrose and isomaltose intolerance results from deficiency of the enzymatic activities of the sucrase-isomaltase complex involved in starch digestion. SpeculationMalabsorption of starch can be expected to result from: (/) deficiency of pancreatic amylase; (2) congenital or acquired deficiency of the glucosidase activities of the brush border; (3) glucose malabsorption; (4) higher intestinal flow rate. The results in this study show that one particular form of starch malabsorption is a consequence of congenital deficiency of the activities of the sucrase-isomaltase complex involved in starch digestion. The methods used here should prove to be useful also in the investigation of other forms of starch malabsorption.

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Section: Methodsmentioning

confidence: 99%

Intraluminal and Mucosal Starch Digestion in Congenital Deficiency of Intestinal Sucrase and Isomaltase Activities

et al. 1972

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“…Following gel filtration of AIr and CIr, the samples that eluted at the bed volume ofthe column in both cases (AIrB and CIrB, respectively), were analyzed by the TLC system of Weill and Hanke (31 (29). Separation of partially methylated alditol acetates was carried out using the gas chromatographic method of Darvill et al (8).…”

Section: Materiais and Methodsmentioning

confidence: 99%

Identification of Polysaccharide Hydrolases Involved in Autolytic Degradation of Zea Cell Walls

Nock

Smith²

1987

Plant Physiol.

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Cell walls of Zea mays (cv LG.II) seedings labeled with 14C were treated with a-mylase from Bacilkusblis to remove starch and mixed linkage gcn. These walls released arbinose, xylose, gctose, and galaturonic acid in addition to glucose when they were allowed to autolyze. Methyftion analysis was perforned on samples of wall which had been bated atolyicaly and the results indicated that degrdation of the majr polymer of the walL the gluconrabinoxylan, had occurred. A amber of glyausses could be dissociated from the wall by use of 3 M LiCL. The proteins which were released were found to contain a number of exogly cia activities in addition to being effective in degradi'ng the polysaccharide substrates, araban, xyan, glatn, laminarin, ma_nn, and polygkawouic acid. The effects of thee enzmes on the wall during antolysis appear to resul from endo-activity in addion to exo-activity. The structu chags that occurred in the cell wall during autolysis were found to be related to the changes previously fomud to occur in cell walls duing auxin induced extenson.It is generally accepted that auxin induced elongation growth involves a biochemical niodification ofexisting cell wall resulting in loosening. Both synthesis (2,3) and degradation (9) have been proposed as responsible for loosening but the critical step leading to such changes in the rheological properties of the wall has yet to be recognized.Undoubtedly synthesis of new wall polymers is required for continued growth and maintenance of wall structure (2, 4), but the event which initiates loosening must also involve the modification of existing wall (4, 6). The concept of wall loosening through bond cleavage led to proposals that polysaccharide hydrolases are responsible for breaking load bearing bonds in the wall. There are a number of reports of turnover of wall components occumng during growth (eg. 4, 9, 20, 22, 24) enzymes with activity against wall polymers might be expected to achieve cleavage of wall restraining cross links.Of those enzymes with endo-activity it has been mainly glucanolytic ones which have been studied with regard to wall polymer degradation (14, 30) and a role for glucan metabolism in regard to wall loosening has been proposed. Glucan metabolism appears to have an integral role to play in continued wall extension but there are reasons for believing that glucan degradation is not a primary step in wall loosening (see Cleland [6] for discussion). However other sugar residues in addition to glucose are subject to turnover in monocot cell walls. In coleoptiles of Zea for example, auxin induces a loss of sugars from the wall indicating turnover of the polysaccharides (9) and more recently Heyn (11)

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“…A was dried under diminished pressure and suspended in 50 ml of the mixture of n-butanol, ethanol and water 2:1:1 (V/V), and subjected to a cellulose powder (Toyo Roshi A, 100 mesh) column chromatography, (2.5 x 80 cm) and eluted with the same solvent. The eluate, each 5 ml, was examined by thin-layer chromatography according to the method of Weill et al [17]. The eluates containing only one oligosaccharide were combined and evaporated under diminished pressure to dryness.…”

Section: Methodsmentioning

confidence: 99%