Thin layer chromatography of dansyl amino acid derivatives

Deyl, Z.; Rosmus, Jan

doi:10.1016/s0021-9673(01)97453-9

Cited by 123 publications

(16 citation statements)

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“…The electronic configuration of Tb 3 + is 41 8 No quenching of the 5D4 terbium fluorescence was (" served in our work with borate glasses from the ppm range to 6 weight percent [49].…”

Section: Terbiummentioning

confidence: 83%

“…When one considers th at information of this kind can be obtained rapidly from the interior of the undamaged, usually functioning ce ll , one realizes the special importance of spect ral lumi -nescence research techniques in biology. An example of fluorimetric methods in this area is: the fluorescent tags for labeling proteins [6] and amino acids [7][8][9][10] which have proven useful in immunochemistry, virology, bacteriology, parasitology, and mycology.…”

Section: Organic Compoundsmentioning

confidence: 99%

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Inorganic ions in glasses and polycrystalline pellets as fluorescence standard reference materials

Reisfeld¹

1972

J. RES. NATL. BUR. STAN. SECT. A.

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The absorption and fluorescence of inorgan ic glasses and polycrystalline disks doped by heavy metal ions is discuss ed , and their use as fluoresce nce stand ard s is evaluated. The advantages of the glass standards over other media is summarized.The glass standards are divided into two groups (1) glasses doped by trivale nt rare earths such as Gd3 +, Tb3 +, Eu3 +, Sm3 +, and Tm3+ which have narrow band optical s pectra as a result of intraconfig~rational transitions, and (2) glasses and polycrystalline disks doped by ions s uch as Tl+, Pb 2 +, Ce 3 +, and Cu + whi ch have broad spectral bands since th e optical spectra originate from interconfigurationally allowed transitions. Optical and physical parameters , including matrix effects, quantum efficiencies, decay characteristics, Stokes' shifts and spin-orbit versus orbit-lattice interaction s due to the different trans itions wi ll be discussed.Group (1) glasses are suitable for use as standards where a narrow well-defined fluorescence range is required, and group (2) glasses are suitable for use as standards whenever a substance with a wide range of fluorescen ce is measured. Special em phasis will be placed on energy transfer between donor and acceptor ions.

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“…The electronic configuration of Tb 3 + is 41 8 No quenching of the 5D4 terbium fluorescence was (" served in our work with borate glasses from the ppm range to 6 weight percent [49].…”

Section: Terbiummentioning

confidence: 83%

Section: Organic Compoundsmentioning

confidence: 99%

Inorganic ions in glasses and polycrystalline pellets as fluorescence standard reference materials

Reisfeld¹

1972

J. RES. NATL. BUR. STAN. SECT. A.

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“…The HC1 was then removed by evaporation and the residue dissolved in 50% pyridine. Identification of the dansyl amino acid was carried out by thin-layer chromatography on Baker-flex 1 B-F silica gel using chloroform-ethanol-acetic acid in the ratio 38:4:3 (v/v) as the solvent (Deyl and Rosmus, 1965).…”

Section: Methodsmentioning

confidence: 99%

Hemocyanin from the Australian freshwater crayfish Cherax destructor. Characterization of a dimeric subunit and its involvement in the formation of the 25S component

Jeffrey¹,

Shaw²,

Treacy³

1978

Biochemistry

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The molecular weight of a dimeric subunit, M3', isolated from Cherax destructor hemocyanin has been measured by sedimentation equilibrium to be 144 000. Peptide mapping and end-group analysis together with gel electrophoresis show that the dimer consists of two very similar or identical monomers, cross-linked by disulfide bridges. Dissociation of the 25S component of the hemocyanin shows that it contains the dimer and two previously identified monomers, M1 and M2. Its molecular weight is 900 000 by sedimentation equilibrium, and reconstitution studies show that the dimer is essential for its formation. Analysis of the results of polyacrylamide disc gel electrophoresis experiments with the 25S component indicates that it consists of a population of 11 compositional isomers. These all contain one dimeric subunit and ten monomeric subunits, the latter being present in all the combinations of M1 and M2.

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“…Numerous solvent systems have been published for the separation m d identification of the dansyl-amino acids by thin-layer chromatography (16)(17)(18)(19)(20)(21)(22). We have found that, in general, two solvent systems are sufficient to allow identification of the majority of the dansyl-amino acids, if the fist system is selected to give mainly separation of the dmsyl derivatives of the nonpolar amino acids, and the second, mainly separation of the dansyl derivatives of the polar amino acids.…”

Section: Damylekfman Degradationmentioning

confidence: 99%

Gene action in the human haptoglobins. IV. Amino acid sequence studies on the haptoglobin α chains

Black¹,

Dixon²

1970

Can. J. Biochem.

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Alpha chains of human haptoglobin have been prepared from whole haptoglobin of genetic type Hp 2–1 purified from the ascites fluid of a single patient. Amino acid sequence analysis has been carried out on these light chains which represent the products α1S and α2(F,S) of the single genes Hp1S and Hp2(F,S), and are, respectively, 83 and 142 residues in length. The data reported here concern the sequence analysis of two series of tryptic peptides, one obtained by unlimited cleavage of the chains by p-toluene sulfonamido-phenylethyl chloromethyl ketone - trypsin, and the second following limitation of trypsin cleavage to arginyl residues after modification of lysyl residues by trifluoracetylation. The major technique for sequence analysis of these peptides was a modification of the p-dimethylaminonaphthalene-sulfonyl-Edman (dansyl-Edman) procedure employing a new thin-layer chromatographic separation of the dansyl-amino acids. The sequences of these tryptic peptides in conjunction with those of the chymotryptic peptides allowed the unequivocal deduction of portions of the sequence, but the final overlaps were provided by fragments obtained by specific chemical cleavage of the chain at aspartyl residues. The amino acid sequences deduced have documented the occurrence of a partial gene duplication in the gene product α2(F,S) of the Hp2(F,S) gene.

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Thin layer chromatography of dansyl amino acid derivatives

Cited by 123 publications

References 1 publication

Inorganic ions in glasses and polycrystalline pellets as fluorescence standard reference materials

Inorganic ions in glasses and polycrystalline pellets as fluorescence standard reference materials

Hemocyanin from the Australian freshwater crayfish Cherax destructor. Characterization of a dimeric subunit and its involvement in the formation of the 25S component

Gene action in the human haptoglobins. IV. Amino acid sequence studies on the haptoglobin α chains

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