Thin layer chromatography/mass spectrometry

Cheng, Sy-Chyi; Huang, Min‐Zong; Shiea, Jentaie

doi:10.1016/j.chroma.2011.01.077

Cited by 118 publications

(61 citation statements)

References 113 publications

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“…When methanol, a solvent that favored elution of proteins, was used as the solvent, lysozyme signals were detected with low salt interferences ( Figure S2b). However, such large molecules were found to have difficulty being carried through and detected if they were loaded too far away from the tip cusp, similar to the observation in TLC [28,29].…”

Section: Separation Property Of Wooden Tipssupporting

confidence: 58%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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Abstract. Conventional electrospray ionization mass spectrometry (ESI-MS) uses a capillary for sample loading and ionization. Along with the development of ambient ionization techniques, ESI-MS using noncapillary emitters has attracted more interest in recent years. Following our recent report on ESI-MS using wooden tips (Anal. Chem. 83, 8201-8207 (2011)), the technique was further investigated and extended in this study. Our results revealed that the wooden tips could serve as a chromatographic column for separation of sample components. Sequential and exhaustive ionization was observed for proteins and salts on wooden tips with salts ionized sooner and proteins later. Nonconductive materials that contain microchannels/pores could be used as tips for ESI-MS analysis with sample solutions loaded to the sharp-ends only, since rapid diffusion of sample solutions by capillary action would enable the tips to become conductive. Tips of inert materials such as bamboo, fabrics, and sponge could be used for sample loading and ionization, while samples such as tissue, mushroom, and bone could form tips to induce ionization for direct analysis with application of a high voltage.

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Section: Separation Property Of Wooden Tipssupporting

confidence: 58%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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“…In the past, MS analysis had followed the scraping of bands from TLC plates and extraction of the lipid components from the bands ( 34,35 ); this approach has been called "indirect sampling TLC-MS" ( 36 ). There are several disadvantages to this "off-line" TLC-MS, including the low recovery rate from scraping the silica gel, the necessity for additional processing with a column, and the contamination of the analyte by residual silica gel.…”

mentioning

confidence: 99%

Direct analysis of sialylated or sulfated glycosphingolipids and other polar and neutral lipids using TLC-MS interfaces

Park¹,

Zhou²,

Costello

2014

Journal of Lipid Research

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“…To sum up, TLC was one of the way to perform multiple detection in situ for (i) physical detection, (ii) microchemical detection, and (Morlock and Schwack 2010) or off-line (Cheng et al 2011). However, associating TLC with Fourier transform infra-red (FTIR) or Raman spectroscopy remain possible approaches.…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%