Thin-layer chromatography for thyroid hormones

The interpretation of the serum protein-bound iodine: A review

“…Thin-layer chromatographic methods, of which several have been published (Taurog, 1963;West, Wayne, and Chavre, 1965;Sakurada, 1965 andRow, Volpe, and Ezrin, 1966;Coenegracht and Postmes, 1967;Favino, Emrich, and von zur Miihlen, 1967;Hoppe, Zappi, and Gries, 1967;Weinert et al, 1967;Fisher, 1968), appear to be the most sensitive but have not been proven in the routine laboratory. A method of separating T3 from T4 by gas-liquid chromatography has been described (Jaakonmaki and Stouffer, 1967), but the preparation of suitable derivatives of T3 and T4 appears to be too laborious for use in a routine laboratory.…”

Prolonged Intravenous Infusion of Labelled Iodocompounds in the Rat: [125i]iodide Metabolism and Effects of Moderate Dietary Iodine Deficiency

“…The purified upper layer was used as the developing solvent. The use of 6 M-ammonia, rather than 2 M-ammonia (West et al 1965), made possible the separation of all principal iodocompounds and 'ageing' the solvent greatly improved the resolution between T3 and I-and, to a lesser extent, between T3 and T4. With the 'aged' solvent, good discrimination between I-(RF~0 -65), T3 (0-48), T4 (0-39), reverse-T3 (0-31) (a standard of 3,3',5'-tri-iodo-L-thyronine (reverse-T3) was not available at the time of the infusion studies, but subsequent t.l.c.…”

“…plate. The developing solvent (modified from West, Wayne & Chavré, 1965) was prepared by mixing ethyl acetate, methanol and 6 M-ammonia in the ratio 5:2:3 (by vol.). The solvent was 'aged' over a period of a few days by repeatedly shaking vigorously then allowing to settle at room temperature into two layers, the lower of which was discarded each time.…”

Comparison of Paper and Thin-Layer Radiochromatography for the Quantitative Analysis of Thyroidal Iodoamino Acids

“…Recently thin-layer chromatography (TLC) has been recommended by Hollingsworth, Dillard & Bondy (1963) and West, Wayne & Chavré (1965) for its speed, convenience and good separation. In addition to the improved separation it has been claimed that the method offers good recovery and reproducibility (Sofianides, Meloni, Alger & Canary, 1966) and that there is less deiodination than with paper (Shapiro & Gordon, 1965).…”