Thin-Layer Chromatography: Fingerprint Analysis of Plant Materials

Agatonović-Kuštrin, Snežana; Morton, David W.

doi:10.1016/b978-0-12-409547-2.14024-7

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…TLC has become a powerful analytical method for analyzing complex samples with advances in instrumentation and the use of modern digital image evaluation techniques that can combine different images of the same fingerprint for a reliable evaluation. Thus, TLC was included as a method of identification of medicinal plants based on a chromatographic imprint in most monographs in the pharmacopoeia [ 1 ]. Interpretation of chromatographic data is performed by combining TLC with image analysis (IA) and chemometric methods.…”

Section: Resultsmentioning

confidence: 99%

“…For these reasons, increased efforts are being made to effectively regulate the medicinal plant industry, which has led to the need to develop accurate and reliable methods for the quality control and standardization of plant material. For the identification of plant materials, specifically their extracts, three approaches are used, namely: the marker approach, the multi-component approach and the model or fingerprint approach [ 1 ]. The use of fingerprints, which are chemical profiles of different constituents in plant material, is widely accepted as an effective way to describe the complexity of the components present in a plant because, in most cases, a comprehensive chemical profile is generally unavailable [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle

Baumli,

Antal,

Casoni

et al. 2023

Plants

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In recent years, the interest in natural remedies has increased, so it is important to analyze the plants widely distributed in nature but whose composition is little known. The main objective of the present work is to obtain information based on the profiles of secondary metabolites and antioxidant activity in Lamium album, a very widespread but little studied plant, with the aim of revealing the differences compared to Urtica dioica. First, the optimization of enzymatic extraction assisted by ultrasound was carried out by the Box–Behnken method. The optimized parameters were: concentration of the enzyme—3.3% cellulase, temperature—55 °C, and the extraction time—40.00 min. The efficiency was estimated based on the content of iridoids, the main class of secondary metabolites from Lamium album. Second, the secondary metabolites profiles of the nettle extracts were obtained by thin-layer chromatography using both normal and reverse phases and by RP-UHPLC. The antioxidant activity was evaluated using DPPH and ABTS+ radicals. The obtained results revealed significant differences between the two nettle species, both in terms of the phytochemical compounds, as well as the antioxidant activity, confirming the fact that Lamium album has a high potential to be used in phytomedicine.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle

Baumli,

Antal,

Casoni

et al. 2023

Plants

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“…As medicinal information was passed on orally, relatively little was known about the therapeutic potential of white sage until recently. HPTLC is an ideal method for chromatographic plant extract fingerprinting, due to its simplicity and speed, low solvent use, and minimal or no sample preparation [9]. Moreover, many samples can be run on the same plate for comparison.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Lactic Acid Fermentation on Extraction of Phenolics and Flavonoids from Sage Leaves

et al. 2022

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This work analysed the effect of spontaneous fermentation of sage leaves on the release and extraction of flavonoid and phenolic compounds. Chemical profiling based on thin-layer chromatography was used to compare different extracts from two sage species, common sage (Salvia officinalis) and white sage (Salvia apiana). Non-fermented Salvia apiana extracts are richer in antioxidants, phenolics, and terpenoids. Fermentation significantly enhances extraction of total phenolics, flavonoids, and antioxidants only from Salvia officinalis leaves, while it does not affect extraction from Salvia apiana leaves. In each 20 µL of extract, extraction of polyphenolics increases from 6.55 to 21.01 µg gallic acid equivalents (GAE), anti-oxidants from 0.68 to 2.12 µg GAE, and flavonoid content from 48.01 to 65.33 µg RE (rutin equivalents). Higher antioxidant activity in fermented Salvia apiana ethyl acetate extracts is associated with an increased concentration of phenolics and phenolic terpenoids. However, in Salvia officinalis, the higher antioxidant activity of fermented extract is a result of the release and improved extraction of flavonoids, as there is no increase in the extraction of phenolics. Lactic acid produced via fermentation and proline from Salvia officinalis leaves forms a natural deep eutectic solvent (NADES), which significantly increases the solubility of flavonoids.

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DoE-assisted development and validation of a thin layer chromatography method for optimized separation of major cannabinoids in Cannabis sativa L. samples

Souza

Limberger

Henriques

2021

Journal of Liquid Chromatography & Related Technologies

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Thin-Layer Chromatography: Fingerprint Analysis of Plant Materials

Cited by 3 publications

References 29 publications

Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle

Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle

The Effect of Lactic Acid Fermentation on Extraction of Phenolics and Flavonoids from Sage Leaves

DoE-assisted development and validation of a thin layer chromatography method for optimized separation of major cannabinoids in Cannabis sativa L. samples

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