Thin-layer chromatography — Direct bioautography for the screening of antimicrobial properties of plant extracts

Jesionek, Wioleta; Grzelak, Edyta M.; Majer‐Dziedzic, Barbara; Choma, Irena

doi:10.1556/jpc.26.2013.2.1

Cited by 27 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many volatile compounds are known to be strong antimicrobials (Elgaali et al, 2002;Tzortzakis, 2007;Singh, 2011;Ć avar et al, 2013;Jesionek et al, 2013;Zheng et al, 2013). The fact that a combination of shorter biochar reactor residence times + lower temperatures resulted in a general trend of greater EHEC inactivation could possibly relate to biochar volatiles.…”

Section: Discussionmentioning

confidence: 99%

Inactivation ofE. coliO157:H7 in Cultivable Soil by Fast and Slow Pyrolysis-Generated Biochar

GurtlerJoshua

BoatengAkwasi

HanYanxue

et al. 2014

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

An exploratory study was performed to determine the influence of fast pyrolysis (FP) and slow pyrolysis (SP) biochars on enterohemorrhagic Escherichia coli O157:H7 (EHEC) in soil. Soil + EHEC (inoculated at 7 log colony-forming units [CFU]/g of soil) + 1 of 12 types of biochar (10% total weight:weight in soil) was stored at 22°C and sampled for 8 weeks. FP switchgrass and FP horse litter biochars inactivated 2.8 and 2.1 log CFU/g more EHEC than no-biochar soils by day 14. EHEC was undetectable by surface plating at weeks 4 and 5 in standard FP switchgrass, FP oak, and FP switchgrass pellet biochars. Conversely, EHEC populations in no-biochar control samples remained as high as 5.8 and 4.0 log CFU/g at weeks 4 and 5, respectively. Additionally, three more SP hardwood pellet biochars (generated at 500°C for 1 h, or 2 h, or generated at 700°C for 30 min) inactivated greater numbers of EHEC than did the no-biochar control samples during weeks 4 and 5. These results suggest that biochar can inactivate E. coli O157:H7 in cultivable soil, which might mitigate risks associated with EHEC contamination on fresh produce.

show abstract

Section: Discussionmentioning

confidence: 99%

Inactivation ofE. coliO157:H7 in Cultivable Soil by Fast and Slow Pyrolysis-Generated Biochar

GurtlerJoshua

BoatengAkwasi

HanYanxue

et al. 2014

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

show abstract

“…TLC seems to be an ideal method for hyphenation with bioautographic detection because of an open layer enabling solvent evaporation, and the possibility of separating many samples in parallel. Besides classical TLC, high-performance thin-layer chromatography (HPTLC), overpressured-layer chromatography (OPLC) and planar electro-chromatography (PEC) can also be easily linked to bioautography [12][13][14][15][16][17][18].…”

Section: Open Accessmentioning

confidence: 99%

“…The tests based on these bacteria were fully validated in our laboratory and used for screening antibiotics in milk [19][20][21][22]. Recently, we have also started to use them in plant analysis [13,14,23]. Antibacterial properties of plant constituents were checked against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Xanthomonas campestris pv.…”

Section: Tlc-db (Thin-layer Chromatography-direct Bioautography)mentioning

confidence: 99%

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

Choma

Jesionek

2015

Chromatography

Self Cite

View full text Add to dashboard Cite

The richness of bioactive compounds in plant materials encourages continuous development of separation methods and bioassays for their isolation and identification. Thin-layer chromatography-direct bioautography links separation on the adsorbent layer with biological tests performed directly on it. Therefore, the method is very convenient for searching plant constituents with biological activity, such as antibiotics. Test bacteria grow directly on a plate surface excluding places where antibacterials are located. They can be detected with reagents converted by living bacteria. TLC-DB is a high throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both screening and semi-quantitative analysis is possible. The targeted compounds can be identified using spectroscopic methods, mostly mass spectrometry, that can be performed directly on a TLC plate. This paper discusses all above mentioned aspects of TLC-DB, illustrating them with literature, schemes and our own results.

show abstract

“…Thin layer chromatography‐direct bioautography (TLC‐DB) overcomes these limitations by applying a bacterial suspension directly onto the TLC plate . ZoIs of bacterial growth are visualised using metabolic stains, typically tetrazolium salts such as 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) or 2‐(4‐iodophenyl)‐3‐(4‐nitrophenyl)‐5‐phenyl‐2 H ‐tetrazolium chloride (INT) .…”

Section: Introductionmentioning

confidence: 99%

“…18 Thin layer chromatography-direct bioautography (TLC-DB) overcomes these limitations by applying a bacterial suspension directly onto the TLC plate. 19 ZoIs of bacterial growth are visualised using metabolic stains, typically tetrazolium salts such as 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT). [20][21][22][23][24] These salts are reduced by dehydrogenases in viable cells to produce purple formazan, thus ZoIs are visualised as white spots on a purple background.…”

Section: Introductionmentioning

confidence: 99%

Characterisation and screening of antimicrobial essential oil components against clinically important antibiotic‐resistant bacteria using thin layer chromatography‐direct bioautography hyphenated with GC‐MS, LC‐MS and NMR

Owen

White

Laird

2018

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction The antimicrobial activity of many essential oils (EOs) is well established, indicating that EOs may be a source of compounds for antimicrobial drug development. Thin layer chromatography‐direct bioautography (TLC‐DB) can quickly identify antimicrobial components in complex mixtures and can be applied to the screening of EOs for lead compounds. Objectives This study aimed to identify antimicrobial components of oregano, rosewood and cumin EOs against antibiotic‐sensitive and ‐resistant bacteria using TLC‐DB and a multi‐faceted approach of GC‐MS, LC‐MS and NMR techniques to characterise bioactive compounds. The study also aimed to quantify the antimicrobial activity of bioactive compounds in order to evaluate their potential for the development of therapies against antibiotic‐resistant bacteria. Materials and Methods EOs were eluted on TLC plates and sprayed with a suspension of Staphylococcus aureus, Enterococcus faecium, Escherichia coli or Pseudomonas aeruginosa (antibiotic‐sensitive and ‐resistant isolates). Zones of inhibition, visualised with iodonitrotetrazolium chloride, were subject to GC‐MS, LC‐MS and NMR to characterise the bioactive compounds. Results Seven compounds were identified from the three EOs using GC‐MS, while LC‐MS and NMR failed to detect the presence of any further non‐volatile or heat labile compounds. Carvacrol was most antimicrobial compound identified, with minimum inhibitory concentrations ranging 0.99–31.62 mM. Conclusion The identified antimicrobial compounds present in oregano, rosewood and cumin EOs including carvacrol may be candidates for the development of novel antimicrobial therapies against antibiotic‐resistant bacteria.

show abstract

Thin-layer chromatography — Direct bioautography for the screening of antimicrobial properties of plant extracts

Cited by 27 publications

References 24 publications

Inactivation ofE. coliO157:H7 in Cultivable Soil by Fast and Slow Pyrolysis-Generated Biochar

Inactivation ofE. coliO157:H7 in Cultivable Soil by Fast and Slow Pyrolysis-Generated Biochar

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

Characterisation and screening of antimicrobial essential oil components against clinically important antibiotic‐resistant bacteria using thin layer chromatography‐direct bioautography hyphenated with GC‐MS, LC‐MS and NMR

Contact Info

Product

Resources

About