Thin-layer chromatography and chemometric analysis in the fingerprinting of selected<i>Scutellaria</i>species

Hawrył, Mirosław; Hawrył, Anna; Świeboda, Ryszard; Niemiec, Małgorzata; Waksmundzka‐Hajnos, Monika

doi:10.1556/1006.2016.29.4.3

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…Hawryl et al studied seven different Scutellaria species using TLC combined with chemometrics analysis. 32 The qualitative characterization involved identifying fifteen compounds, among which was luteolin. The aim of this article was the fingerprint analysis of extracts from different species of the genus Scutellaria to estimate the identity of various plant materials by TLC and subsequent PCA.…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

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Analytical determinations of luteolin

Tesio¹,

Robledo

2021

BioFactors

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Plants, through the photosynthesis process, produce the substances necessary for all the life cycles of nature, which are called "primary metabolites." Moreover, there are some plants that synthesize, in addition to these, other substances with more specific functions, which are known as "secondary metabolites." It is inside this group that flavonoids are located, whose main function is to protect organisms from damage caused by different oxidizing agents. Luteolin (3,4,5,7-tetrahydroxy-flavone) belongs to the sub-class of flavonoids known as flavones and is one of 10,000 flavonoids currently known,

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Section: Thin-layer Chromatographymentioning

confidence: 99%

“…Hawryl et al . studied seven different Scutellaria species using TLC combined with chemometrics analysis 32 . The qualitative characterization involved identifying fifteen compounds, among which was luteolin.…”

Section: Chromatographic Determinationsmentioning

confidence: 99%

Analytical determinations of luteolin

Tesio¹,

Robledo

2021

BioFactors

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“…Total flavonoids were separated through the use of n-Butanol and sequentially analyzed by silica gel 60F 254 (15 mg) and thin layer chromatography (TLC; 5 µm, 20 × 20 cm). The chromatogram was transferred to MeOH-H 2 O (70:30), CHCl 3 -MeOH (75:25), and BuOH-CH 3 COOH-H 2 O (16:28:56) as a solvent system (Olennikov et al, 2010;Hawryl et al, 2016). Spots were detected Sigma Chemical; in methanol, followed by 5% solution of polyethylene glycol 400 in ethanol) under ultraviolet-366 nm (Rahman, 2005).…”

Section: Chromatography Sectionmentioning

confidence: 99%

Acta Biologica Cracoviensia s. Botanica

Dehkordi¹,

Kharazian²,

Lorigooini³

2020

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The genus Scutellaria L., Lamiaceae family, Scutellarioideae sub-family is known as the most important medicinal plant in the world. This research aimed to investigate the flavonoid content of seven Scutellaria species from the center, southwest, and west of Iran. Via thin layer and column chromatography, the flavonoid was extracted from leaves and then purified. To screen the flavonoid compositions, a chromatographic method was applied by liquid chromatography mass spectrometry on a triple quadrupole mass spectrometer (LC/TQMS/MS). Fingerprint analysis was implemented so as to characterize a total of 73 chemical compounds, from which 71 compounds were flavonoids belonging to different classes. Flavone with 37 derivatives possessed major values. The most abundant flavonoid compounds were observed in S. multicaulis (23 compounds) and S. patonii (22 compounds). Flavonoid composition, including apigenin, kaempferol, quercetin, and hydroxylflavones represented remarkable derivatives. A total of 45 flavonoids, one tannin and one anthraquinone compound, were observed to be primarily separated and identified for Scutellaria species. Moreover, six categorized chemical groups were identified in this genus and proposed as chemical barcodes. The specific chemical groups strongly provided the boundaries of Scutellaria species, the pharmacological value enhancement, breeding programs, and comprehensive documents of the species. According to the results, LC/TQMS/MS was proven a dominant method regarding genus Scutellaria.

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Thin-Layer Chromatography for Evaluation of Herbal Drugs

Mukherjee

2019

Quality Control and Evaluation of Herbal Drugs

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Thin-layer chromatography and chemometric analysis in the fingerprinting of selectedScutellariaspecies

Cited by 10 publications

References 13 publications

Analytical determinations of luteolin

Analytical determinations of luteolin

Acta Biologica Cracoviensia s. Botanica

Thin-Layer Chromatography for Evaluation of Herbal Drugs

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