Thin-layer chromatographic determination of procainamide and N-acetylprocainamide in human serum and urine at single-dose levels

Kark, B.; Sistovaris, N.; Keller, A. J.

doi:10.1016/s0378-4347(00)84843-4

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Procainamide, an anti-arrhythmic drug, is also metabolized mainly by N-acetylation (Kark et al, 1983). Both fast and slow acetylators can develop drug-induced SLE as a result of procainamide therapy, but slow acetylators are more prone to the condition (Reidenberg, 1981).…”

Section: Introductionmentioning

confidence: 99%

Metabolites of procainamide and practolol inhibit complement components C3 and C4

Sim

Stanley

Gill

et al. 1988

Biochemical Journal

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Drug-induced systemic lupus erythematosus arises from toxic side-effects of administration of hydralazine, isoniazid, procainamide and practolol. Hydralazine and isoniazid are nucleophilic drugs and inhibit the covalent binding reaction of complement components, C3 and C4, an effect likely to lead to deposition of immune complexes (a feature of systemic lupus erythematosus). Procainamide and practolol do not themselves inhibit C3 and C4. A range of metabolites and putative metabolites of procainamide and practolol were synthesized, and tested for their ability to inhibit the covalent binding reactions of C3 and C4. The highly nucleophilic hydroxylamine metabolite of procainamide was strongly inhibitory in both tests, as was a putative hydroxylamine metabolite of practolol. These studies indicate a potential role for the hydroxylamine metabolites in mediating the toxic side-effects of procainamide and practolol, and emphasize the need for adequate measurements of hydroxylamine metabolites in human tissue.

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Section: Introductionmentioning

confidence: 99%

Metabolites of procainamide and practolol inhibit complement components C3 and C4

Sim

Stanley

Gill

et al. 1988

Biochemical Journal

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“…A small number of methods has been devised to quantitate PA and NAPA simultaneously in plasma by Thin Layer Chromatography (TLC) [ 13 , 14 ], Gas-Liquid Chromatography (GLC) [ 15 ], and HPLC [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ], but most lack sensitivity and/or specificity. Although several UV-HPLC methods achieved sensitivity and specificity improvements, they require multistep sample preparations that involve Liquid–Liquid Extraction (LLE), evaporation, and reconstitution.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Procainamide and N-acetylprocainamide in Rat Plasma by Ultra-High-Pressure Liquid Chromatography Coupled with a Diode Array Detector and Its Application to a Pharmacokinetic Study in Rats

et al. 2018

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A simple, sensitive, and reliable reversed-phase, Ultra-High-Pressure Liquid Chromatography (UHPLC) coupled with a Diode Array Detector (DAD) method for the simultaneous determination of Procainamide (PA) and its major metabolite, N-acetylprocainamide (NAPA), in rat plasma was developed and validated. A simple deproteinization method with methanol was applied to the rat plasma samples, which were analyzed using UHPLC equipped with DAD at 280 nm, and a Synergi™ 4 µm polar, reversed-phase column using 1% acetic acid (pH 5.5) and methanol (76:24, v/v) as eluent in isocratic mode at a flow rate 0.2 mL/min. The method showed good linearity (r2 > 0.998) over the concentration range of 20–100,000 and 20–10,000 ng/mL for PA and NAPA, respectively. Intra- and inter-day accuracies ranged from 97.7 to 110.9%, and precision was <10.5% for PA and 99.7 to 109.2 and <10.5%, respectively, for NAPA. The lower limit of quantification was 20 ng/mL for both compounds. This is the first report of the UHPLC-DAD bioanalytical method for simultaneous measurement of PA and NAPA. The most obvious advantage of this method over previously reported HPLC methods is that it requires small sample and injection volumes, with a straightforward, one-step sample preparation. It overcomes the limitations of previous methods, which use large sample volume and complex sample preparation. The devised method was successfully applied to the quantification of PA and NAPA after an intravenous bolus administration of 10 mg/kg procainamide hydrochloride to rats.

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A novel, molecularly imprinted polymer sensor made using an oligomeric methyl silsesquioxane–TiO2 composite sol on a glassy carbon electrode for the detection of procainamide hydrochloride

Wang

Guan

Chai

et al. 2015

Biosensors and Bioelectronics

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Thin-layer chromatographic determination of procainamide and N-acetylprocainamide in human serum and urine at single-dose levels

Cited by 6 publications

References 28 publications

Metabolites of procainamide and practolol inhibit complement components C3 and C4

Metabolites of procainamide and practolol inhibit complement components C3 and C4

Simultaneous Determination of Procainamide and N-acetylprocainamide in Rat Plasma by Ultra-High-Pressure Liquid Chromatography Coupled with a Diode Array Detector and Its Application to a Pharmacokinetic Study in Rats

A novel, molecularly imprinted polymer sensor made using an oligomeric methyl silsesquioxane–TiO2 composite sol on a glassy carbon electrode for the detection of procainamide hydrochloride

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