Thin-Layer Chromatographic Competitive Protein-Binding Assay for Cortisol and Cortisone, and its Application to Urine Samples from Healthy Men Undergoing Water Diuresis

Comment on “Diurnal excretion of urinary cortisol, cortisone, and cortisol metabolites in chronic fatigue syndrome”

“…If one takes his so-called bcorticosteroidsQ as UFC and UFCn, it clear that UFCn (the main urinary free glucocorticosteroid) excretion depends on urine volume. This is corroborated by previous literature showing a significant positive relation between UFCn excretion and urine volume [4,11,12]. Thus, interpretation of the UFCn values presented by Jerjes et al [1] is difficult or even impossible if fluid intake is not strictly controlled [12].…”

Simultaneous determination of urinary cortisol, cortisone and corticosterone in parachutists, depressed patients and healthy controls in view of biomedical and pharmacokinetic studies

“…The common separation protocols for quantification and identification of glucocorticoids in urine include immunoassay (IA), [53][54][55] liquid chromatography (LC), 16,29,32,46,48,[56][57][58][59][60] liquid chromatography coupled to mass spectrometry (LC-MS), [61][62][63][64][65] gas chromatography coupled to mass spectrometry (GC-MS), 66,67 thin-layer chromatography (TLC) 68,69 as well as capillary electrophoretic (CE) techniques, in particular its mode micellar electrokinetic chromatography (MEKC). 30,[70][71][72][73][74][75] IA-based methods are widely used for glucocorticoid analysis because of their simplicity, sensitivity and short analysis time.…”

High Cognitive Dietary Restraint Is Associated With Increased Cortisol Excretion in Postmenopausal Women: A Comment

“…They observed that cortisone, a major metabolite of UFF in the urine, demonstrated a crossreactivity of 44%, and they concluded that this method overestimates urinary cortisol, giving results up to twice those obtained by a specific gas chromatography-mass spectrometry method. Thus, we may assume that Rideout and colleagues (1) measured the sum of free cortisol and urinary free cortisone (UFE) excretion [for discussion, see (3)], and that the observed changes were mainly due to changes of UFE excretion; this may especially be true if urine volume is increased [see also (4,5)]. Thus, their finding ''we were surprised to find a relatively strong relationship between mean total water intake and cortisol excretion'' [(1), p. 632] should be changed as follows: ''we were surprised to find a relatively strong relationship between mean total water intake and corticosteroid (UFF 1 UFE) excretion.''…”